安定細胞株製品 » Mouse Recombinant 4-1BB Stable Cell Line
CHO-K1/mouse 4-1BB Stable Cell Line

Figure 1: FACS analysis of cell surface expression of mouse 4-1BB on CHO-K1/mouse 4-1BB cells. The CHO-K1/mouse 4-1BB cells (blue) and the negative control CHO-K1 cells (red) were probed using Mouse IgG control (Whole Molecule), Purified (Cat. No. A01007, GenScript) and [PE] conjugated Goat anti Mouse IgG (H+L) (Cat. No. A10087, GenScript).

CHO-K1/mouse 4-1BB Stable Cell Line

Recombinant CHO-K1 cells stably overexpress tumor necrosis factor receptor superfamily member 9 isoform 1 precursor (Mus musculus) on the surface. The surface expression of mouse 4-1BB is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse 4-1BB and evaluating target binding affinity.
M00568
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Overview
Product Description Recombinant CHO-K1 cells stably overexpress tumor necrosis factor receptor superfamily member 9 isoform 1 precursor (Mus musculus) on the surface. The surface expression of mouse 4-1BB is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse 4-1BB and evaluating target binding affinity.
Applications Cell-based binding assay or use as immunogen

Product Introduction
Expressed Gene Codon optimized from NM_011612.2; no expressed tags
Target Protein NP_035742
Host Cell CHO-K1
Quantity 2 vials of frozen cells (1x106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile. 

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms ILA; Ly63; 4-1BB; Cd137; CDw137; TNFRSF9
Background 4-1BB is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), CD137 and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule.
4-1BB can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, 4-1BB expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Examples
  • CHO-K1/mouse 4-1BB Stable Cell Line
    • CHO-K1/mouse 4-1BB Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of mouse 4-1BB on CHO-K1/mouse 4-1BB cells. The CHO-K1/mouse 4-1BB cells (blue) and the negative control CHO-K1 cells (red) were probed using Mouse IgG control (Whole Molecule), Purified (Cat. No. A01007, GenScript) and [PE] conjugated Goat anti Mouse IgG (H+L) (Cat. No. A10087, GenScript).