FACS analysis of mouse PD-L1 expression in CHO-K1/mouse PD-L1 cells.
| M00567 | |
|
|
|
| ¥1,613,997.00 | |
|
|
|
|
|
|
| Ask us a question | |
| Product Description |
Recombinant CHO-K1 cells stably overexpress
mouse programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface
expression of PD-L1 is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene | Codon optimized from NM_021893.3; no expressed tags |
| Target Protein | NP_068693.1 |
| Host Cell | CHO-K1 |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318,
Lonza). |
| Stability |
Stable through more than 16 passages
without significant changes in assay performance or expression profile. |
| Culture Properties | Adherent |
| Freeze Medium | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin
(Cat. No. A11138-03, Gibco) |
| Gene Synonyms | CD274; B7-H1; B7H1; PDCD1L1; PDCD1LG1; PDL1 |
| Background |
Programmed death-ligand 1 (PD-L1) also known as cluster of
differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans
is encoded by the CD274 gene. The formation of PD-1 receptor / PD-L1 or B7.1
receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the
proliferation of these CD8+ T cells at the lymph nodes and supplementary to that
PD-1 is also able to control the accumulation of foreign antigen specific T
cells in the lymph nodes through apoptosis which is further mediated by a lower
regulation of the gene Bcl-2 |
FACS analysis of mouse PD-L1 expression in CHO-K1/mouse PD-L1 cells.
