安定細胞株製品 » Human Recombinant OX40 Stable Cell Line
CHO-K1/OX40 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human OX40 on CHO-K1/OX40 cells. The CHO-K1/OX40 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human OX40 antibody (Cat. No. 350004, Biolegend).

CHO-K1/OX40 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human OX40 on CHO-K1/OX40 cells. The CHO-K1/OX40 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human OX40 antibody (Cat. No. 350004, Biolegend).

CHO-K1/OX40 Stable Cell Line

OX40 (CD134) is a member of the TNFR/TNF superfamily. Costimulatory signals from OX40 to a conventional T cell promotes division and survival, augmenting the clonal expansion of effector and memory populations as they are being generated to antigens. OX40 additionally suppresses the differentiation and activity of Treg, further amplifying this process.
M00561
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human OX40 on the surface. The surface expression of OX40 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against OX40 or to measure binding affinity between OX40 and anti-OX40 antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/OX40 Stable Cell Line
    • CHO-K1/OX40 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human OX40 on CHO-K1/OX40 cells. The CHO-K1/OX40 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human OX40 antibody (Cat. No. 350004, Biolegend).

  • CHO-K1/OX40 Stable Cell Line
    • CHO-K1/OX40 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human OX40 on CHO-K1/OX40 cells. The CHO-K1/OX40 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human OX40 antibody (Cat. No. 350004, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.