
Figure 1. Amylin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY2 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with AMY2 agonist, amylin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of amylin (mean ± SEM, n = 3). The EC50 of amylin on CHO-K1/Gα15/AMY2 cells was 51.69 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.
CHO-K1/Gα15/AMY2 Stable Cell Line
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