安定細胞株製品 » CHO-K1/Gα15/AMY1 Stable Cell Line
CHO-K1/Gα15/AMY1 Stable Cell Line

Figure 1. α-CGRP induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY1 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with agonist α-CGRP. The intracellular calcium change was measured by FLIPR. The fluorescence signal were normalized and plotted against the log of the cumulative doses (5-fold dilution) of α-CGRP (Mean ± SD, n = 4). The EC50 of α-CGRP on this cell was 58.8 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/Gα15/AMY1 Stable Cell Line

CHO-K1/Gα15/AMY1 Stable Cell Line

Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin, calcitonin (CT), glucagon, and vasoactive intestinal peptide (VIP).There are three distinct types of RAMPs, designated RAMP1, RAMP2, and RAMP3, each encoded by a separate gene._x000D_ Currently the function of RAMPs is divided into 2 class activities. Association of RAMPs with either the CT or CALCRL proteins forms 6 different receptors from the calcitonin receptor family. When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL), RAMPs can change the selectivity of the receptor for a specific hormone. In the cases of the other receptors mentioned however, there is no evidence that they can do this, but instead function to regulate trafficking of receptors from the ER / golgi to the membrane. _x000D_ GenScript's cloned human AMY1–expressing cell line is generated in the CHO-K1/Gα15 host._x000D_
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Description

Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin, calcitonin (CT), glucagon, and vasoactive intestinal peptide (VIP).There are three distinct types of RAMPs, designated RAMP1, RAMP2, and RAMP3, each encoded by a separate gene._x000D_ Currently the function of RAMPs is divided into 2 class activities. Association of RAMPs with either the CT or CALCRL proteins forms 6 different receptors from the calcitonin receptor family. When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL), RAMPs can change the selectivity of the receptor for a specific hormone. In the cases of the other receptors mentioned however, there is no evidence that they can do this, but instead function to regulate trafficking of receptors from the ER / golgi to the membrane. _x000D_ GenScript's cloned human AMY1–expressing cell line is generated in the CHO-K1/Gα15 host._x000D_
Synonyms

Gene Synonyms: RAMP1+CALCR

Overview
Applications Functional assay for AMY1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/AMY1 Stable Cell Line
    • CHO-K1/Gα15/AMY1 Stable Cell Line

    Figure 1. α-CGRP induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY1 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with agonist α-CGRP. The intracellular calcium change was measured by FLIPR. The fluorescence signal were normalized and plotted against the log of the cumulative doses (5-fold dilution) of α-CGRP (Mean ± SD, n = 4). The EC50 of α-CGRP on this cell was 58.8 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/Gα15/AMY1 Stable Cell Line
    • CHO-K1/Gα15/AMY1 Stable Cell Line