安定細胞株製品 » Human Recombinant CXCR4 Receptor Stable Cell Line
CHO-K1/CXCR4/Gα15 Stable Cell Line

Figure 1. h-CXCL12b/SDF-1β-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR4 cells. The cells were loaded with Calcium-4 prior to stimulation with a CXCR4 receptor agonist h-CXCL12b/SDF-1β. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of h-CXCL12b/SDF-1β (Mean ± SD, n = 2). The EC50 of h-CXCL12b/SDF-1β on this cells was 1.05 µg/ml.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/CXCR4/Gα15 Stable Cell Line

CHO-K1/CXCR4/Gα15 Stable Cell Line

CXCR4 is a receptor for the C-X-C chemokine SDF-1 (Stromal Cell-Derived Factor 1). It is involved in haematopoiesis and cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, cerebellar development and survival of hippocampal-neuron of central nerve system. CXCR4 also acts as a primary receptor for some HIV-2 isolates and as a co-receptor with CD4 for HIV-1 X4 viruses.
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Description

CXCR4 is a receptor for the C-X-C chemokine SDF-1 (Stromal Cell-Derived Factor 1). It is involved in haematopoiesis and cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, cerebellar development and survival of hippocampal-neuron of central nerve system. CXCR4 also acts as a primary receptor for some HIV-2 isolates and as a co-receptor with CD4 for HIV-1 X4 viruses.
Synonyms

CXCR4; CD184

Overview
Applications Functional assay for hCXCR4 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery.

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml puromycin (Cat. #A11138-03, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/CXCR4/Gα15 Stable Cell Line
    • CHO-K1/CXCR4/Gα15 Stable Cell Line

    Figure 1. h-CXCL12b/SDF-1β-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR4 cells. The cells were loaded with Calcium-4 prior to stimulation with a CXCR4 receptor agonist h-CXCL12b/SDF-1β. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of h-CXCL12b/SDF-1β (Mean ± SD, n = 2). The EC50 of h-CXCL12b/SDF-1β on this cells was 1.05 µg/ml.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/CXCR4/Gα15 Stable Cell Line
    • CHO-K1/CXCR4/Gα15 Stable Cell Line