安定細胞株製品 » CHO-K1/4-1BB
CHO-K1/4-1BB Stable Cell Line

Figure 1. FACS analysis of cell surface expression of 4-1BB on CHO-K1/4-1BB cells. The CHO-K1/4-1BB cells (Blue) and the negative control CHO-K1 cells (Red) were probed using 4-1BB Antibody (Cat. No. A01974, Genscript) and PE conjugated Goat anti Mouse IgG (H+L) (Cat. No. ab6785, Abcam).

CHO-K1/4-1BB Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human TNF receptor superfamily member 9 (4-1BB) on the surface. The surface expression of 4-1BB is validated by FACS analysis.
M00538
¥1,613,997.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human TNF receptor superfamily member 9 (4-1BB) on the surface. The surface expression of 4-1BB is validated by FACS analysis.
Applications Binding assay or used as immunogen.

Product Introduction
Expressed Gene Codon optimized from NM_001561.5; no expressed tags
Target Protein NP_001552.2
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms TNFRSF9, ILA; 4-1BB; CD137; CDw137; IMD109
Background CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule.

Examples
  • CHO-K1/4-1BB Stable Cell Line
    • CHO-K1/4-1BB Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of 4-1BB on CHO-K1/4-1BB cells. The CHO-K1/4-1BB cells (Blue) and the negative control CHO-K1 cells (Red) were probed using 4-1BB Antibody (Cat. No. A01974, Genscript) and PE conjugated Goat anti Mouse IgG (H+L) (Cat. No. ab6785, Abcam).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.