安定細胞株製品 » CHO-K1/VISTA
CHO-K1/VISTA Stable Cell Line

Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).

CHO-K1/VISTA Stable Cell Line

Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
M00533
¥1,613,997.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
Applications Cell-based binding assay or use as immunogen

Product Introduction
Expressed Gene Codon optimized from NM_022153.1; no expressed tags
Target Protein NP_071436
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1×106 per vial in 1 ml) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% Complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms B7H5, GI24, B7-H5, Dies1, PD-1H, SISP1, VISTA, PP2135, C10orf54, DD1alpha
Background V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells. VISTA levels are heightened within the tumor microenvironment, in which its blockade can enhance antitumor immune responses in mice. In humans, blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials. Here, we report the structure of human VISTA and examine its function in lymphocyte negative regulation in cancer.

Examples
  • CHO-K1/VISTA Stable Cell Line
    • CHO-K1/VISTA Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).