安定細胞株製品 » CHO-K1/VISTA
CHO-K1/VISTA Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green) were probed using PE-conjugated anti-human VISTA antibody (Cat. No. FAB71261P, R&D Systems).

CHO-K1/VISTA Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green) were probed using PE-conjugated anti-human VISTA antibody (Cat. No. FAB71261P, R&D Systems).

CHO-K1/VISTA Stable Cell Line

Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
M00533
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human V-set immunoregulatory receptor (VISTA) on the surface. The surface expression of VISTA is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against VISTA or to measure binding affinity between VISTA and anti-VISTA antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/VISTA Stable Cell Line
    • CHO-K1/VISTA Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green) were probed using PE-conjugated anti-human VISTA antibody (Cat. No. FAB71261P, R&D Systems).

  • CHO-K1/VISTA Stable Cell Line
    • CHO-K1/VISTA Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green) were probed using PE-conjugated anti-human VISTA antibody (Cat. No. FAB71261P, R&D Systems).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.