安定細胞株製品 » CHO-K1/Tim3
CHO-K1/Tim3 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human TIM3 on CHO-K1/TIM3 cells. The CHO-K1/TIM3 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD366 antibody (Cat. No. 364805, Biolegend).

CHO-K1/Tim3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human hepatitis A virus cellular receptor 2 (TIM3) on the surface. The surface expression of TIM3 is validated by FACS analysis.
M00531
¥1,613,997.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human hepatitis A virus cellular receptor 2 (TIM3) on the surface. The surface expression of TIM3 is validated by FACS analysis.
Applications Binding assay or use as immunogen

Product Introduction
Expressed Gene NCBI reference sequence NM_032782.4; no expressed tags
Target Protein NP_116171.3
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)

Background
Gene Synonyms HAVCR2, CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3
Background T cell immunoglobulin mucin-3 (TIM-3) is a member of the T-cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. TIM-3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280 amino acid mature human TIM-3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171 amino acid extracellular region, which shares 60% amino acid identity with mouse TIM-3 ECD.

Examples
  • CHO-K1/Tim3 Stable Cell Line
    • CHO-K1/Tim3 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human TIM3 on CHO-K1/TIM3 cells. The CHO-K1/TIM3 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD366 antibody (Cat. No. 364805, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.