安定細胞株製品 » Human chemokine (C-X-C motif) receptor 5 (CXCR5) Stable Cell Line
CHO-K1/Gα15/CXCR5

Figure BCA-1 induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist BCA-1 (GenScript, Cat No.: Z02826). The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of BCA-1 (Mean ± SD, n = 2). The EC50 of BCA on this cell was 0.11 µM.

CHO-K1/Gα15/CXCR5

Chemokine (C-X-C motif) receptor 5 (CXCR5) belongs to the CXC chemokine receptor family. CXCR5 plays crucial roles in B-cells migration into B-cell follicles of spleen and Peyer patches. CXCR5 reduces maintenance of immature neural cell populations and enhances proliferation of subgranular zone cells in the hippocampal dentate gyrus. GenScript’s human CCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
M00526
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Description

Chemokine (C-X-C motif) receptor 5 (CXCR5) belongs to the CXC chemokine receptor family. CXCR5 plays crucial roles in B-cells migration into B-cell follicles of spleen and Peyer patches. CXCR5 reduces maintenance of immature neural cell populations and enhances proliferation of subgranular zone cells in the hippocampal dentate gyrus. GenScript’s human CCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
Synonyms

BLR1; CD185; MDR15

Overview
Applications Functional assay for CXCR5 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CXCR5
    • CHO-K1/Gα15/CXCR5

    Figure BCA-1 induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist BCA-1 (GenScript, Cat No.: Z02826). The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of BCA-1 (Mean ± SD, n = 2). The EC50 of BCA on this cell was 0.11 µM.