安定細胞株製品 » Human chemokine (C-X-C motif) receptor 5 (CXCR5) Stable Cell Line
CHO-K1/Gα15/CXCR5

Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.

CHO-K1/Gα15/CXCR5

Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.

CHO-K1/Gα15/CXCR5

Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00526
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/Gα15/CXCR5
    • CHO-K1/Gα15/CXCR5

    Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.

  • CHO-K1/Gα15/CXCR5
    • CHO-K1/Gα15/CXCR5

    Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.