Figure 1. CXCL11-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR3 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR3 agonist, CXCL11 (Cat. No. Z03246, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CXCL11 (mean ± SEM, n = 3). The EC₅₀ of CXCL11 on CHO-K1/Gα15/CXCR3 cells was 0.66 μg/ml.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CXCL9 in CHO-K1/Gα15/CXCR3 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/Gα15/CXCR3 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CXCL9 on CHO-K1/Gα15/CXCR3 cells was 3.26 μg/ml.

Figure 3. FACS analysis of cell surface expression of human CXCR3 on CHO-K1/Gα15/CXCR3 cells. The CHO-K1/Gα15/CXCR3 cells (green) and the negative control CHO-K1 cells (orange) were probed using anti-human CD182 IgG1 antibody and Alexa Fluor 647-conjugated goat anti-human IgG (H+L) antibody (Cat. No. A21445, Invitrogen).

CHO-K1/Gα15/CXCR3

Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 3 (CXCR3) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.

Cat. No.	M00525
Size	2 vials : Quote Buy in bulk
Quantity
Price	¥1,661,121.00


Ask us a question

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 3 (CXCR3) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 20 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples

Figure 1. CXCL11-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR3 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR3 agonist, CXCL11 (Cat. No. Z03246, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CXCL11 (mean ± SEM, n = 3). The EC₅₀ of CXCL11 on CHO-K1/Gα15/CXCR3 cells was 0.66 μg/ml.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CXCL9 in CHO-K1/Gα15/CXCR3 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/Gα15/CXCR3 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CXCL9 on CHO-K1/Gα15/CXCR3 cells was 3.26 μg/ml.

Figure 3. FACS analysis of cell surface expression of human CXCR3 on CHO-K1/Gα15/CXCR3 cells. The CHO-K1/Gα15/CXCR3 cells (green) and the negative control CHO-K1 cells (orange) were probed using anti-human CD182 IgG1 antibody and Alexa Fluor 647-conjugated goat anti-human IgG (H+L) antibody (Cat. No. A21445, Invitrogen).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CHO-K1/Gα15/CXCR3

サポート

会社情報

サポート

会社情報