安定細胞株製品 » Human chemokine (C-X-C motif) receptor 3 (CXCR3A) Stable Cell Line
CHO-K1/Gα15/CXCR3

Figure ITAC-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR3 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist ITAC. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of ITAC (Mean ± SD, n = 2). The EC50 of ITAC on this cell was 0.26 µg/ml. P1, P5, P10 and P20 represent the cell passage number.

CHO-K1/Gα15/CXCR3

CHO-K1/Gα15/CXCR3

Chemokine (C-X-C motif) receptor 3 (CXCR3) belongs to the CXC chemokine receptor family. Similar to other chemokine receptors, it is a G protein-coupled receptor (GPCR). CXCR3 has two distinct splice variants, CXCR3A and CXCR3B, demonstrating different binding affinities. CXCR3-A binds to the CXC chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC) while CXCR3-B can also bind to CXCL4 in addition to CXCL9, CXCL10, and CXCL11. GenScript’s human CXCR3A-expressing stable subline is guaranteed to function properly in calcium flux assay.
M00525
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Description

Chemokine (C-X-C motif) receptor 3 (CXCR3) belongs to the CXC chemokine receptor family. Similar to other chemokine receptors, it is a G protein-coupled receptor (GPCR). CXCR3 has two distinct splice variants, CXCR3A and CXCR3B, demonstrating different binding affinities. CXCR3-A binds to the CXC chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC) while CXCR3-B can also bind to CXCL4 in addition to CXCL9, CXCL10, and CXCL11. GenScript’s human CXCR3A-expressing stable subline is guaranteed to function properly in calcium flux assay.

Synonyms

GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R

Overview
Applications Functional assay for CXCR3 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CXCR3
  • CHO-K1/Gα15/CXCR3

    Figure ITAC-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR3 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist ITAC. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of ITAC (Mean ± SD, n = 2). The EC50 of ITAC on this cell was 0.26 µg/ml. P1, P5, P10 and P20 represent the cell passage number.

  • CHO-K1/Gα15/CXCR3
  • CHO-K1/Gα15/CXCR3