安定細胞株製品 » Human chemokine (C-X-C motif) receptor 1 (CXCR1) Stable Cell Line
CHO-K1/Gα15/CXCR1

Figure IL-8 induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CXCR1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist IL-8. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of IL-8 (Mean ± SD, n = 2). The EC50 of IL-8 on this cell was 0.11 µg/ml.

CHO-K1/Gα15/CXCR1

Chemokine (C-X-C motif) receptor 1 (CXCR1) is a rhodopsin-like G protein-coupled receptor. It is one of two high-affinity receptors for the CXC chemokine interleukin-8 (IL-8), a major mediator of immune and inflammatory responses. The structure of human CXCR1 in a lipid bilayer should help to facilitate the discovery of new compounds that interact with GPCRs. GenScript’s human CXCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
M00524
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Description

Chemokine (C-X-C motif) receptor 1 (CXCR1) is a rhodopsin-like G protein-coupled receptor. It is one of two high-affinity receptors for the CXC chemokine interleukin-8 (IL-8), a major mediator of immune and inflammatory responses. The structure of human CXCR1 in a lipid bilayer should help to facilitate the discovery of new compounds that interact with GPCRs. GenScript’s human CXCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
Synonyms

C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1

Overview
Applications Functional assay for CXCR1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CXCR1
    • CHO-K1/Gα15/CXCR1

    Figure IL-8 induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CXCR1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist IL-8. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of IL-8 (Mean ± SD, n = 2). The EC50 of IL-8 on this cell was 0.11 µg/ml.