安定細胞株製品 » Human chemokine (C-X-C motif) receptor 1 (CXCR1) Stable Cell Line
CHO-K1/Gα15/CXCR1

Figure 1. IL-8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR1 agonist, IL-8 (Cat. No. Z03138, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of IL-8 (mean ± SEM, n = 3). The EC50 of IL-8 on CHO-K1/Gα15/CXCR1 cells was 0.11 μg/ml.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/Gα15/CXCR1

Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 1 (CXCR1) on the surface and contain high levels of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
M00524
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 1 (CXCR1) on the surface and contain high levels of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/Gα15/CXCR1
  • CHO-K1/Gα15/CXCR1

    Figure 1. IL-8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR1 agonist, IL-8 (Cat. No. Z03138, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of IL-8 (mean ± SEM, n = 3). The EC50 of IL-8 on CHO-K1/Gα15/CXCR1 cells was 0.11 μg/ml.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.