安定細胞株製品 » Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line
CHO-K1/Gα15/CCR5

Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR5-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR5 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P10, and P20 represent the cell passage number.

CHO-K1/Gα15/CCR5

C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.
M00523
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Description

C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.
Synonyms

CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Overview
Applications Functional assay for CCR5 receptor

Product Introduction
Storage Liquid nitrogen immediately upon receiving
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 µg/ml puromycin (Cat. #A11138-03, Gibco), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/Gα15/CCR5
    • CHO-K1/Gα15/CCR5

    Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR5-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR5 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P10, and P20 represent the cell passage number.