安定細胞株製品 » Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line
CHO-K1/Gα15/CCR5

Figure 1. CCL3-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CCR5 agonist, CCL3 (Cat. No. Z03137, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CCL3 (mean ± SEM, n = 3). The EC50 of CCL3 on CHO-K1/Gα15/CCR5 cells was 0.28 μg/ml.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/Gα15/CCR5

Figure 2. FACS analysis of cell surface expression of human CCR5 on CHO-K1/Gα15/CCR5 cells. The CHO-K1/Gα15/CCR5 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD195 antibody (Cat. No. 359122, Biolegend).

CHO-K1/Gα15/CCR5

Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 5 (CCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00523
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 5 (CCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS.
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/Gα15/CCR5
    • CHO-K1/Gα15/CCR5

    Figure 1. CCL3-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CCR5 agonist, CCL3 (Cat. No. Z03137, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CCL3 (mean ± SEM, n = 3). The EC50 of CCL3 on CHO-K1/Gα15/CCR5 cells was 0.28 μg/ml.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

  • CHO-K1/Gα15/CCR5
    • CHO-K1/Gα15/CCR5

    Figure 2. FACS analysis of cell surface expression of human CCR5 on CHO-K1/Gα15/CCR5 cells. The CHO-K1/Gα15/CCR5 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD195 antibody (Cat. No. 359122, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.