安定細胞株製品 » Human Recombinant Gastric Inhibitory Polypeptide Receptor Stable Cell Line
CHO-K1/GIP/Gα15 Stable Cell Line

Figure 1. GIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GIP/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GIP. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GIP (Mean ± SD, n = 2). The EC50 of GIP on this cell was 0.12 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/GIP/Gα15 Stable Cell Line

Gastric inhibitory polypeptide receptor (GIPR) is a Gs-coupled GPCR which is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. Its ligand GIP is secreted after meal ingestion has been shown to stimulate bone formation resulting in lower occurrences of osteoporosis. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.
M00486
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Description

Gastric inhibitory polypeptide receptor (GIPR) is a Gs-coupled GPCR which is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. Its ligand GIP is secreted after meal ingestion has been shown to stimulate bone formation resulting in lower occurrences of osteoporosis. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.
Synonyms

GIP receptor, Gippr, RATGIPPR, GIP-R, gastric inhibitory peptide receptor, gastric inhibitory polypeptide receptor, glucose-dependent insulinotropic polypeptide receptor, LOC232937, LOC381853, Gm1081, Gm160

Overview
Applications Functional assay for GIP receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/GIP/Gα15 Stable Cell Line
    • CHO-K1/GIP/Gα15 Stable Cell Line

    Figure 1. GIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GIP/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GIP. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GIP (Mean ± SD, n = 2). The EC50 of GIP on this cell was 0.12 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom