安定細胞株製品 » Human Recombinant Neuropeptide Y Receptor Y2 Stable Cell Line
CHO-K1/NPY2/Gqi5 Stable Cell Line

Figure 1. NPY-induced concentration-dependent stimulation of intracellular calcium mobilization in CHOK1/NPY2/Gqi5 cells. The cells were loaded with Calcium-4 prior to stimulation with a NPY2 receptor agonist, NPY. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NPY (Mean ± SD, n = 2). The EC50 of NPY on this cell was 5.5 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/NPY2/Gqi5 Stable Cell Line

CHO-K1/NPY2/Gqi5 Stable Cell Line

The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. With regard to endogenous agonists, the receptors Y2 preferentially bind NPY and PYY.
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Description

The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. With regard to endogenous agonists, the receptors Y2 preferentially bind NPY and PYY.
Synonyms

Y2 receptor, neuropeptide Y receptor Y2, neuropeptide Y receptor Y2-like, neuropeptide Y receptor type 2, neuropeptide Y-Y2 receptor, neuropeptide Y/peptide YY-Y2 receptor, NPY-Y2 receptor

Overview
Applications Functional assay for NPY2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/NPY2/Gqi5 Stable Cell Line
    • CHO-K1/NPY2/Gqi5 Stable Cell Line

    Figure 1. NPY-induced concentration-dependent stimulation of intracellular calcium mobilization in CHOK1/NPY2/Gqi5 cells. The cells were loaded with Calcium-4 prior to stimulation with a NPY2 receptor agonist, NPY. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NPY (Mean ± SD, n = 2). The EC50 of NPY on this cell was 5.5 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/NPY2/Gqi5 Stable Cell Line
    • CHO-K1/NPY2/Gqi5 Stable Cell Line