安定細胞株製品 » Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line
CHO-K1/GLP1R/Gα15 Stable Cell Line

Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1R/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GLP-1 (7-37). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1 (7-37) on this cell was 0.14 µM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/GLP1R/Gα15 Stable Cell Line

Figure 2. FACS analysis of cell surface expression of GLP1R on CHO-K1/GLP1R/Gα15 stable cell line. The fluorescence signal of the target protein on cell product (red) was compared to negative control (green) and detected with GLP1R Antibody (Cat. No. A01826, GenScript) followed by FITC conjugated Goat anti-Mouse IgG Antibody (H+L) (Cat. No. ab6785, Abcam).

CHO-K1/GLP1R/Gα15 Stable Cell Line

Figure 3. Dose dependent stimulation of intracellular cAMP mobilization upon treatment with calcitonin in CHO-K1/GLP1R/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/GLP1R/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Varioskan, Thermo Fisher). The EC50 of GLP-1 (7-37) was 1.84 nM.

CHO-K1/GLP1R/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human glucagon-like peptide-1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00451
¥1,613,997.00

Ask us a question
Overview
Product Description Recombinant CHO-K1 cells stably overexpress human glucagon-like peptide-1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Applications Calcium flux assay, FACS, cAMP accumulation assay.

Product Introduction
Expressed Gene Codon optimized from NM_002062; no expressed tags
Target Protein NP_002053
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

Background
Gene Synonyms GLP-1, GLP-1-R, GLP-1R
Background GLP-1R binds specifically the glucagon-like peptide-1 (GLP-1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP-1R is known to be expressed in pancreatic beta cells. Activated GLP-1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP-1R has been suggested as a potential target for the treatment of diabetes. GLP-1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP-1R display improved memory and learning. 

Examples
  • CHO-K1/GLP1R/Gα15 Stable Cell Line
    • CHO-K1/GLP1R/Gα15 Stable Cell Line

    Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1R/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GLP-1 (7-37). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1 (7-37) on this cell was 0.14 µM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/GLP1R/Gα15 Stable Cell Line
    • CHO-K1/GLP1R/Gα15 Stable Cell Line

    Figure 2. FACS analysis of cell surface expression of GLP1R on CHO-K1/GLP1R/Gα15 stable cell line. The fluorescence signal of the target protein on cell product (red) was compared to negative control (green) and detected with GLP1R Antibody (Cat. No. A01826, GenScript) followed by FITC conjugated Goat anti-Mouse IgG Antibody (H+L) (Cat. No. ab6785, Abcam).

  • CHO-K1/GLP1R/Gα15 Stable Cell Line
    • CHO-K1/GLP1R/Gα15 Stable Cell Line

    Figure 3. Dose dependent stimulation of intracellular cAMP mobilization upon treatment with calcitonin in CHO-K1/GLP1R/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/GLP1R/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Varioskan, Thermo Fisher). The EC50 of GLP-1 (7-37) was 1.84 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.