安定細胞株製品 » Human Recombinant PAR2 Proteinase-activated Receptor Stable Cell Line
CHO-K1/PAR2/Gα15 Stable Cell Line

Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist trypsin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of trypsin (Mean ± SD, n = 2). The EC50 of Trypsin on this cell was 1.7 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/PAR2/Gα15 Stable Cell Line

Proteinase-activated receptors (PAR) are a subfamily of G-protein coupled, seven-transmembrane domain receptors, which are cleaved within the aminoterminal exodomain by certain serine proteinases at a specific peptide bond. Trypsin and mast cell tryptase, and more recently, the activated coagulation factors VIIa and Xa, have been identified as serine proteinases able to activate mammalian PAR-2. As already indicated, PAR-2 is believed to be involved in inflammation. This role for PAR-2 implies that elastase and cathepsin G would paradoxically display an anti-inflammatory property by disarming PAR-2.
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Description

Proteinase-activated receptors (PAR) are a subfamily of G-protein coupled, seven-transmembrane domain receptors, which are cleaved within the aminoterminal exodomain by certain serine proteinases at a specific peptide bond. Trypsin and mast cell tryptase, and more recently, the activated coagulation factors VIIa and Xa, have been identified as serine proteinases able to activate mammalian PAR-2. As already indicated, PAR-2 is believed to be involved in inflammation. This role for PAR-2 implies that elastase and cathepsin G would paradoxically display an anti-inflammatory property by disarming PAR-2.
Synonyms

PAR2, PAR-2, GPR11, proteinase-activated receptor-2, Proteinase-activated receptor-2 G protein-coupled receptor 11, Proteinase-activated receptor-2, G protein-coupled receptor 11, coagulation factor II receptor-like 1, proteinase-activated receptor 2, thrombin receptor-like 1, Protease-activated receptor-2, Gpcr11

Overview
Applications Functional assay for PAR2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 µg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 µg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/PAR2/Gα15 Stable Cell Line
    • CHO-K1/PAR2/Gα15 Stable Cell Line

    Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist trypsin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of trypsin (Mean ± SD, n = 2). The EC50 of Trypsin on this cell was 1.7 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom