
Figure 1. Sodium acetate-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FFA2/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist sodium acetate. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of sodium acetate (Mean ± SEM, n = 3). The EC50 of sodium acetate on this cell was 0.38 mM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with sodium acetate in CHO-K1/FFA2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/FFA2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of sodium acetate on CHO-K1/FFA2/Gα15 cells was 0.31 mM.
CHO-K1/FFA2/Gα15 Stable Cell Line
M00437 | |
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¥1,661,121.00 | |
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