安定細胞株製品 » Human Recombinant Prostanoid Receptor DP2 Stable Cell Line
CHO-K1/DP2/Gα15 Stable Cell Line

Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC50 of PGD2 on this cell was 15 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/DP2/Gα15 Stable Cell Line

Prostaglandin D(2) (PGD(2)) is derived from arachidonic acid and binds with high affinity to the G-protein coupled receptors prostanoid DP(1) and DP(2). Interaction with DP(2) results in cell chemotaxis, eosinophil degranulation, eosinophil shape change, adhesion molecule upregulation and Th2 cytokine production. In allergic rhinitis and allergic asthma PGD(2) is released from mast cells in response to allergen challenge and may trigger symptoms such as sneezing, rhinorrhea, pruritus, mucus hypersecretion and pulmonary inflammation. GenScript's cloned human DP2-expressing cell line co-expressing Gα15 is made in the CHO-K1 host.
M00436
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Description

Prostaglandin D(2) (PGD(2)) is derived from arachidonic acid and binds with high affinity to the G-protein coupled receptors prostanoid DP(1) and DP(2). Interaction with DP(2) results in cell chemotaxis, eosinophil degranulation, eosinophil shape change, adhesion molecule upregulation and Th2 cytokine production. In allergic rhinitis and allergic asthma PGD(2) is released from mast cells in response to allergen challenge and may trigger symptoms such as sneezing, rhinorrhea, pruritus, mucus hypersecretion and pulmonary inflammation. GenScript's cloned human DP2-expressing cell line co-expressing Gα15 is made in the CHO-K1 host.
Synonyms

DP2 receptor, CRTH2, GPR44, CD294, DP2, G protein-coupled receptor 44, chemoattractant receptor homologous molecule expressed on T helper type 2 cells, Gpr44_predicted, LOC309212, G protein-coupled receptor 44 (predicted), prostaglandin D2 receptor 2, PGD2 receptor

Overview
Applications Functional assay for DP2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F12, 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 3 μg/ml puromycin (Cat. #A11138-03, Gibco)

Examples
  • CHO-K1/DP2/Gα15 Stable Cell Line
    • CHO-K1/DP2/Gα15 Stable Cell Line

    Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC50 of PGD2 on this cell was 15 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom