安定細胞株製品 » Human Recombinant Cannabinoid Receptor 2 Stable Cell Line
CHO-K1/CB2 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/CB2 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/CB2 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of CP-55940 on CHO-K1/CB2 cells was 57.84 nM

CHO-K1/CB2 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/CB2 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/CB2 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of CP-55940 on CHO-K1/CB2 cells was 57.84 nM

CHO-K1/CB2 Stable Cell Line

The Cannabinoid receptor 2 (CNR2) is a Gi/o-coupled GPCR expressed in spleen, tonsils, bone marrow and peripheral blood leukocytes. There is one report that cannabinoid-induced inhibition of helper T cell activation is lost in macrophages obtained from CNR2 knockout mice. CNR2 is a potential therapeutic target in the treatment of various disease conditions, such as pain, multiple sclerosis, vascular disease, Parkinson's disease, and other central nerve system disorders.
M00433
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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human cannabinoid receptor 2 (CB2) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stability: Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/CB2 Stable Cell Line
    • CHO-K1/CB2 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/CB2 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/CB2 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of CP-55940 on CHO-K1/CB2 cells was 57.84 nM

  • CHO-K1/CB2 Stable Cell Line
    • CHO-K1/CB2 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/CB2 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/CB2 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of CP-55940 on CHO-K1/CB2 cells was 57.84 nM


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.