安定細胞株製品 » Human Recombinant Bile Acid Receptor 1 Stable Cell Line
CHO-K1/GPBAR1 Stable Cell Line

Figure 1. LCA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/GPBAR1 cells. The intracellular cAMP change was measured by Varioskan. The ratio of the luminescence signal were plotted against the log of the cumulative doses of LCA (Mean ± SD, n = 2). The EC50 of LCA on this cell was 6.06 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/GPBAR1 Stable Cell Line

The gene GPBRA1 encodes G protein-coupled bile acid receptor. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production.
M00432
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Description

The gene GPBRA1 encodes G protein-coupled bile acid receptor. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production.
Synonyms

GPBA receptor, BG37, M-BAR, hGPCR19, TGR5, GPCR19, GPR131, MGC40597, GPBAR1, GPCR, G protein-coupled bile acid receptor 1, G protein-coupled receptor, membrane-type receptor for bile acids

Overview
Applications Functional assay for GPBAR1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 µg/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/GPBAR1 Stable Cell Line
    • CHO-K1/GPBAR1 Stable Cell Line

    Figure 1. LCA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/GPBAR1 cells. The intracellular cAMP change was measured by Varioskan. The ratio of the luminescence signal were plotted against the log of the cumulative doses of LCA (Mean ± SD, n = 2). The EC50 of LCA on this cell was 6.06 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.