安定細胞株製品 » Human Recombinant P2Y6 Purinergic Receptor Stable Cell Line
1321N1/P2Y6 Stable Cell Line

Figure 1: UDP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y6 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y6 receptor agonist, UDP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UDP (Mean ± SEM, n = 3). The EC50 of UDP on 1321N1/P2Y6 cells was 75.49 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y6 Stable Cell Line

Figure 1: UDP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y6 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y6 receptor agonist, UDP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UDP (Mean ± SEM, n = 3). The EC50 of UDP on 1321N1/P2Y6 cells was 75.49 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y6 Stable Cell Line

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Recombinant 1321N1 cells stably overexpress human pyrimidinergic receptor P2Y6 (P2Y6) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
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Product Introduction
Product Description Recombinant 1321N1 cells stably overexpress human pyrimidinergic receptor P2Y6 (P2Y6) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stability: Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 100 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM, 10% FBS
Freeze Medium-DATA 45% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

Examples
  • 1321N1/P2Y6 Stable Cell Line
    • 1321N1/P2Y6 Stable Cell Line

    Figure 1: UDP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y6 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y6 receptor agonist, UDP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UDP (Mean ± SEM, n = 3). The EC50 of UDP on 1321N1/P2Y6 cells was 75.49 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • 1321N1/P2Y6 Stable Cell Line
    • 1321N1/P2Y6 Stable Cell Line

    Figure 1: UDP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y6 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y6 receptor agonist, UDP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UDP (Mean ± SEM, n = 3). The EC50 of UDP on 1321N1/P2Y6 cells was 75.49 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.