安定細胞株製品 » Human Recombinant Glucagon Receptor Stable Cell Line
CHO-K1/GCGR/Gα15 Stable Cell Line

Figure 1. Glucagon-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GCGR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist glucagon. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Glucagon (Mean ± SD, n = 2). The EC50 of Glucagon on this cell was 0.46 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50 -X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/GCGR/Gα15 Stable Cell Line

CHO-K1/GCGR/Gα15 Stable Cell Line

Glucagon regulates blood glucose via control of hepatic glycogenolysis and gluconeogenesis and via regulation of insulin release from the β cell. Pharmacological administration of glucagon increases blood glucose in normal and diabetic subjects, and produces positive inotropic and chronotropic cardiovascular effects, relaxation of smooth muscle in the gastrointestinal tract and stimulation of growth hormone secretion. The actions of glucagon are mediated via a single adenylate cyclase-coupled glucagon receptor that also couples to the phospholipase C-inositol phosphate (PLC-IP) pathway leading to Ca2+ release from intracellular stores.
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Description

Glucagon regulates blood glucose via control of hepatic glycogenolysis and gluconeogenesis and via regulation of insulin release from the β cell. Pharmacological administration of glucagon increases blood glucose in normal and diabetic subjects, and produces positive inotropic and chronotropic cardiovascular effects, relaxation of smooth muscle in the gastrointestinal tract and stimulation of growth hormone secretion. The actions of glucagon are mediated via a single adenylate cyclase-coupled glucagon receptor that also couples to the phospholipase C-inositol phosphate (PLC-IP) pathway leading to Ca2+ release from intracellular stores.
Synonyms

glucagon receptor, GGR, MGC93090, GL-R, glucagon receptor perhaps same as Niddm3, GR

Overview
Applications Functional assay for glucagon receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/GCGR/Gα15 Stable Cell Line
    • CHO-K1/GCGR/Gα15 Stable Cell Line

    Figure 1. Glucagon-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GCGR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist glucagon. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Glucagon (Mean ± SD, n = 2). The EC50 of Glucagon on this cell was 0.46 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50 -X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/GCGR/Gα15 Stable Cell Line
    • CHO-K1/GCGR/Gα15 Stable Cell Line