安定細胞株製品 » Human Recombinant MC2 Melanocortin Receptor Stable Cell Line
CHO-K1/MC2/Gα15 Stable Cell Line

Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2).The EC50 of ACTH on this cell was 0.13 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/MC2/Gα15 Stable Cell Line

The melanocortin receptor 2, MC2 receptor, is Gs-coupled GPCRs expressed in zona fasciculata of the adrenal cortex placental and stimulates production of cortisol. MC2 is a member of the rhodopsin family of 7-transmembrane and it's also known as the ACTH receptor or corticotropin receptor because it is specific for ACTH alone. Activation of the MC2 receptor initiates a cascade of events affecting multiple steps in corticoid steroidogenesis. Mutations in MC2 may result in familial glucocorticoid deficiency, a group of autosomal recessive disorders characterized by resistance to ACTH.
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Description

The melanocortin receptor 2, MC2 receptor, is Gs-coupled GPCRs expressed in zona fasciculata of the adrenal cortex placental and stimulates production of cortisol. MC2 is a member of the rhodopsin family of 7-transmembrane and it's also known as the ACTH receptor or corticotropin receptor because it is specific for ACTH alone. Activation of the MC2 receptor initiates a cascade of events affecting multiple steps in corticoid steroidogenesis. Mutations in MC2 may result in familial glucocorticoid deficiency, a group of autosomal recessive disorders characterized by resistance to ACTH.
Synonyms

MC2 receptor, MC2R, ACTH-R, ACTH, ACTHR, melanocortin 2 receptor, adrenocorticotropic hormone receptor

Overview
Applications Functional assay for MC2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/MC2/Gα15 Stable Cell Line
    • CHO-K1/MC2/Gα15 Stable Cell Line

    Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2).The EC50 of ACTH on this cell was 0.13 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.