安定細胞株製品 » Human Recombinant SST2 Somatostatin Receptor Stable Cell Line
CHO-K1/SST2/Gα15 Stable Cell Line

Figure 1. Somatostatin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist somatostatin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Somatostatin (Mean ± SD, n = 2). The EC50 of somatostatin on this cell was 0.9 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/SST2/Gα15 Stable Cell Line

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney. Studies showed the involvement of the SST2 receptor in the inhibition of glucagon secretion.
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Description

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney. Studies showed the involvement of the SST2 receptor in the inhibition of glucagon secretion.
Synonyms

sst2 receptor, SSTR2, SRIF1, SRIF1A, Smstr2, SRIF-1, SS-2-R, SS2-R, SS2R, somatostatin receptor 2, somatostatin receptor subtype 2, somatostatin receptor type 2, somatotropin release-inhibiting factor receptor, SSTR-2, sst2, Smstr-2

Overview
Applications Functional assay for SST2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 200 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/SST2/Gα15 Stable Cell Line
    • CHO-K1/SST2/Gα15 Stable Cell Line

    Figure 1. Somatostatin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist somatostatin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Somatostatin (Mean ± SD, n = 2). The EC50 of somatostatin on this cell was 0.9 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.