安定細胞株製品 » Human Recombinant 5-HT2C Serotonin Receptor Stable Cell Line
HEK293/5-HT2C Stable Cell Line

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/5-HT2C cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with 5-HT2C agonist, 5-HT (Cat. No. 14927, Sigma Aldrich). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of 5-HT (mean ± SEM, n = 3). The EC50 of 5-HT on HEK293/5-HT2C cells was 0.29 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is % stimulation of ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

HEK293/5-HT2C Stable Cell Line

Recombinant HEK293 cells stably overexpress human 5-hydroxytryptamine receptor 2C (5-HT2C) on the surface and contain high level of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
M00319
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Product Introduction
Product Description Recombinant HEK293 cells stably overexpress human 5-hydroxytryptamine receptor 2C (5-HT2C) on the surface and contain high level of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 300 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10566, Life Technologies), 10% FBS
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • HEK293/5-HT2C Stable Cell Line
    • HEK293/5-HT2C Stable Cell Line

    Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/5-HT2C cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with 5-HT2C agonist, 5-HT (Cat. No. 14927, Sigma Aldrich). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of 5-HT (mean ± SEM, n = 3). The EC50 of 5-HT on HEK293/5-HT2C cells was 0.29 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is % stimulation of ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.