
Figure 1. GHRH (1-29)-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/GHRH/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with GHRHR agonist, GHRH (1-29). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of GHRH (mean ± SEM, n = 3). The EC50 of GHRH on HEK293/GHRH/Gα15 cells was 0.22 μM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.
HEK293/GHRH/Gα15 Stable Cell Line
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