安定細胞株製品 » Human Recombinant Melatonin MT2 Receptor Stable Cell Line
CHO-K1/MT2/Gα15 Stable Cell Line

Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with a MT2 receptor agonist melatonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of melatonin (Mean ± SD, n = 2). The EC50 of melatonin on this cell was 12.75 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/MT2/Gα15 Stable Cell Line

CHO-K1/MT2/Gα15 Stable Cell Line

Melatonin is a neurohormone that plays a key role in the synchronisation of circadian and seasonal functions with cyclic environmental variations. In mammals, two melatonin receptors, MT1 and MT2, have been cloned. Activation of MT2 melatonin receptors phase shift circadian rhythms of neuronal firing in the suprachiasmatic nucleus, inhibit dopamine release in retina, induce vasodilation and inhibition of leukocyte rolling in arterial beds, and enhance immune responses.
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Description

Melatonin is a neurohormone that plays a key role in the synchronisation of circadian and seasonal functions with cyclic environmental variations. In mammals, two melatonin receptors, MT1 and MT2, have been cloned. Activation of MT2 melatonin receptors phase shift circadian rhythms of neuronal firing in the suprachiasmatic nucleus, inhibit dopamine release in retina, induce vasodilation and inhibition of leukocyte rolling in arterial beds, and enhance immune responses.
Synonyms

MT2 receptor, Mel1b, ML1B, Mel1b-r, mel1b melatonin receptor, mel1b receptor, melatonin receptor 1B, melatonin receptor type 1B, Mel1b, Mt2

Overview
Applications Functional assay for MT2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/MT2/Gα15 Stable Cell Line
    • CHO-K1/MT2/Gα15 Stable Cell Line

    Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with a MT2 receptor agonist melatonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of melatonin (Mean ± SD, n = 2). The EC50 of melatonin on this cell was 12.75 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/MT2/Gα15 Stable Cell Line
    • CHO-K1/MT2/Gα15 Stable Cell Line