安定細胞株製品 » Human Recombinant EP2 Prostanoid Receptor Stable Cell Line
CHO-K1/EP2/Gα15 Stable Cell Line

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTGER2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SD, n = 2). The EC50 of PGE2 on this cell was 0.25 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/EP2/Gα15 Stable Cell Line

CHO-K1/EP2/Gα15 Stable Cell Line

Prostaglandin (PG) E2 exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE2 designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and expression regulation. Studies have identified that EP2 mediates many processes, such as facilitating ovulation and fertilization, suppressing dendritic cell differentiation, and promoting amyloid-β formation in Alzheimer's disease.
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Description

Prostaglandin (PG) E2 exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE2 designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and expression regulation. Studies have identified that EP2 mediates many processes, such as facilitating ovulation and fertilization, suppressing dendritic cell differentiation, and promoting amyloid-β formation in Alzheimer's disease.
Synonyms

prostaglandin E, prostaglandin E receptor 2, 53kD, Ptger-ep2, PGE receptor EP2 subtype, prostaglandin E receptor 2, prostaglandin E receptor EP2 subtype, prostaglandin E2 receptor EP2 subtype, prostaglandin E2 receptor EP2 subtype-like, prostaglandin E2 receptor type 2, prostanoid EP2 receptor, Ptgerep2

Overview
Applications Functional assay for EP2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. #10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/EP2/Gα15 Stable Cell Line
    • CHO-K1/EP2/Gα15 Stable Cell Line

    Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTGER2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SD, n = 2). The EC50 of PGE2 on this cell was 0.25 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/EP2/Gα15 Stable Cell Line
    • CHO-K1/EP2/Gα15 Stable Cell Line