安定細胞株製品 » Human Recombinant Prostanoid IP1 Receptor Stable Cell Line
CHO-K1/IP1/Gα15 Stable Cell Line

Figure 1. Iloprost-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/IP1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, Iloprost. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Iloprost (Mean ± SD, n = 2). The EC50 of Iloprost on this cell was 1.1 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/IP1/Gα15 Stable Cell Line

IP1 receptor is a receptor for prostaglandin I2 or prostacyclin. When binding with a prostacyclin molecule, the receptor changes conformation and activates Gs. Prostaglandin I2, the major product of cyclooxygenase in macrovascular endothelium, mediates a potent vasodilation and inhibition of platelet aggregation by binding to this receptor.
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Description

IP1 receptor is a receptor for prostaglandin I2 or prostacyclin. When binding with a prostacyclin molecule, the receptor changes conformation and activates Gs. Prostaglandin I2, the major product of cyclooxygenase in macrovascular endothelium, mediates a potent vasodilation and inhibition of platelet aggregation by binding to this receptor.
Synonyms

IP1 receptor, prostacyclin, IP, prostanoid IP receptor, LOC292661, Ptgir_predicted, PGI receptor, PGI2 receptor, prostacyclin receptor, prostaglandin I receptor (IP), prostaglandin I receptor (IP) (predicted), prostaglandin I2 (prostacyclin) receptor, prostaglandin I2 receptor

Overview
Applications Functional assay for IP1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco).

Examples
  • CHO-K1/IP1/Gα15 Stable Cell Line
    • CHO-K1/IP1/Gα15 Stable Cell Line

    Figure 1. Iloprost-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/IP1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist, Iloprost. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Iloprost (Mean ± SD, n = 2). The EC50 of Iloprost on this cell was 1.1 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.