安定細胞株製品 » Human Recombinant H2 Histamine Receptor Stable Cell Line
CHO-K1/H2/Gα15 Stable Cell Line

Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H2 receptor agonist, Histamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on this cell was 89 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/H2/Gα15 Stable Cell Line

CHO-K1/H2/Gα15 Stable Cell Line

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3, and H4. Specifically, the histamine receptor H2 belongs to the rhodopsin-like family of G protein-coupled receptors. As an integral membrane protein, it stimulates gastric acid secretion and regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation.
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Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3, and H4. Specifically, the histamine receptor H2 belongs to the rhodopsin-like family of G protein-coupled receptors. As an integral membrane protein, it stimulates gastric acid secretion and regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation.
Synonyms

H2 receptor, H2R, HH2R, Histamine H2 receptor

Overview
Applications Functional assay for H2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery.

Culture Conditions
Freeze Medium 45% culture medium,45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/H2/Gα15 Stable Cell Line
    • CHO-K1/H2/Gα15 Stable Cell Line

    Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H2 receptor agonist, Histamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on this cell was 89 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom

  • CHO-K1/H2/Gα15 Stable Cell Line
    • CHO-K1/H2/Gα15 Stable Cell Line