安定細胞株製品 » Human Recombinant P2Y11 Purinergic Receptor Stable Cell Line
1321N1/P2Y11 Stable Cell Line

Figure 1: ATP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y11 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y11 receptor agonist, ATP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ATP (Mean ± SEM, n = 3). The EC50 of ATP on P2Y11 in 1321N1 cells was 0.82 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y11 Stable Cell Line

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Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y11 (P2Y11) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00298
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Product Introduction
Product Description Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y11 (P2Y11) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 250 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10569, Life Technologies), 10% FBS
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • 1321N1/P2Y11 Stable Cell Line
    • 1321N1/P2Y11 Stable Cell Line

    Figure 1: ATP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y11 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a P2Y11 receptor agonist, ATP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ATP (Mean ± SEM, n = 3). The EC50 of ATP on P2Y11 in 1321N1 cells was 0.82 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.