安定細胞株製品 » Human Recombinant SST3 Somatostatin Receptor Stable Cell Line
CHO-K1/SST3/Gα15 Stable Cell Line

Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist somatostatin-14. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on this cell was 0.226 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/SST3/Gα15 Stable Cell Line

Somatostatin receptors (SSTRs), a family of seven transmembrane (TM) domain G-protein-coupled receptors having five distinct subtypes (termed SSTR1-5), are activated by somatostatin secreted from the nerve and endocrine cells. SSTRs are widely expressed in many tissues, frequently as multiple subtypes that coexist in the same cell. With expressions in a tissue-specific manner, SSTRs are involved in the regulation of secretion of insulin, glucagon, and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. The five receptors share common signaling pathways such as the inhibition of adenylyl cyclase, activation of phosphotyrosine phosphatase (PTP), and modulation of mitogen-activated protein kinase (MAPK) through G-protein-dependent mechanisms. Aberrant expression of somatostatin receptors is known to be involved in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR3 mRNA is detected in the brain and pancreatic islets. SSTR3 uniquely triggers PTP-dependent apoptosis accompanied by the activation of p53 and pro-apoptotic protein Bax, and displays acute desensitization of adenylyl cyclase coupling.
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Description

Somatostatin receptors (SSTRs), a family of seven transmembrane (TM) domain G-protein-coupled receptors having five distinct subtypes (termed SSTR1-5), are activated by somatostatin secreted from the nerve and endocrine cells. SSTRs are widely expressed in many tissues, frequently as multiple subtypes that coexist in the same cell. With expressions in a tissue-specific manner, SSTRs are involved in the regulation of secretion of insulin, glucagon, and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. The five receptors share common signaling pathways such as the inhibition of adenylyl cyclase, activation of phosphotyrosine phosphatase (PTP), and modulation of mitogen-activated protein kinase (MAPK) through G-protein-dependent mechanisms. Aberrant expression of somatostatin receptors is known to be involved in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR3 mRNA is detected in the brain and pancreatic islets. SSTR3 uniquely triggers PTP-dependent apoptosis accompanied by the activation of p53 and pro-apoptotic protein Bax, and displays acute desensitization of adenylyl cyclase coupling.
Synonyms

sst3 receptor, SSTR3, SRIF1, SRIF1C, SSTR, Smstr28, SS-3-R, SS3-R, SS3R, SSR-28, somatostatin receptor 28, somatostatin receptor 3, somatostatin receptor subtype 3, somatostatin receptor type 3, sst3, Smstr-3, Smstr3

Overview
Applications Functional assay for SST3 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/SST3/Gα15 Stable Cell Line
    • CHO-K1/SST3/Gα15 Stable Cell Line

    Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist somatostatin-14. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on this cell was 0.226 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom