安定細胞株製品 » Human Recombinant ADRA2A Adrenoceptors Stable Cell Line
CHO-K1/ADRA2A/Gα15 Stable Cell Line

Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC50 of UK14304 on this cell was 2.68 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADRA2A/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 2A (ADRA2A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00281
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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 2A (ADRA2A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Applications binding assay, calcium mobilization assay

Product Introduction
Expressed Gene Codon optimized from NM_000681; no expressed tags
Target Protein NP_000672
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Species Human
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

Background
Gene Synonyms ADRA2A; ADRA2; ADRA2R; ADRAR; ALPHA2AAR; ZNF32
Background The adrenoceptor ADRA2A is expressed in the brain, spleen, kidney, aorta, lung, skeletal muscle, heart and liver. ADRA2A-adrenoceptor knockout mice exhibit disruption of presynaptic inhibition of noradrenaline release at high stimulation frequencies. ADRA2A receptor is the principal autoreceptor in the presynaptic feedback loop regulating noradrenaline release. However, another α2 autoreceptor is also present.

Examples
  • CHO-K1/ADRA2A/Gα15 Stable Cell Line
    • CHO-K1/ADRA2A/Gα15 Stable Cell Line

    Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC50 of UK14304 on this cell was 2.68 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.