安定細胞株製品 » Human Recombinant Adenosine A1 Receptor Stable Cell Line
HEK293/ADORA1/Gα15 Stable Cell Line

Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/ADORA1/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with ADORA1 agonist, NECA (Cat. No. HY-103173, MCE). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of NECA (mean ± SEM, n = 3). The EC50 of NECA on HEK293/ADORA1/Gα15 cells was 4.09 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.

HEK293/ADORA1/Gα15 Stable Cell Line

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Recombinant HEK293 cells stably overexpress human adenosine A1 receptor (ADORA1) on the surface and contain high level of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00255
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Product Introduction
Product Description Recombinant HEK293 cells stably overexpress human adenosine A1 receptor (ADORA1) on the surface and contain high level of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10566, Life Technologies), 10% FBS
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • HEK293/ADORA1/Gα15 Stable Cell Line
    • HEK293/ADORA1/Gα15 Stable Cell Line

    Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/ADORA1/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with ADORA1 agonist, NECA (Cat. No. HY-103173, MCE). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of NECA (mean ± SEM, n = 3). The EC50 of NECA on HEK293/ADORA1/Gα15 cells was 4.09 nM.
    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.