安定細胞株製品 » Human Recombinant D1 Dopamine Receptor Stable Cell Line
CHO-K1/D1 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with dopamine in CHO-K1/D1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/D1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of dopamine on CHO-K1/D1 cells was 53.05 nM.

CHO-K1/D1 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with dopamine in CHO-K1/D1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/D1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of dopamine on CHO-K1/D1 cells was 53.05 nM.

CHO-K1/D1 Stable Cell Line

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4).
M00247
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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human dopamine receptor D1 (D1) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/D1 Stable Cell Line
    • CHO-K1/D1 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with dopamine in CHO-K1/D1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/D1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of dopamine on CHO-K1/D1 cells was 53.05 nM.

  • CHO-K1/D1 Stable Cell Line
    • CHO-K1/D1 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with dopamine in CHO-K1/D1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/D1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of dopamine on CHO-K1/D1 cells was 53.05 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.