安定細胞株製品 » Human Recombinant Adenosine A2A Receptor Stable Cell Line
CHO-K1/ADORA2A/Gα15 Stable Cell Line

Figure 1. NECA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/ADORA2A/Gα15 cells. The cells were stimulated with agonist NECA and the intracellular cAMP change was measured by Pherastar. The ration of fluorescence signals were normalized and plotted against the log of the cumulative doses of NECA (Mean ± SD, n = 2). The EC50 of NECA on this cell was 2.0 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADORA2A/Gα15 Stable Cell Line

The adenosine receptors ADORA2A is Gs-coupled GPCRs expressed in the thymus gland, heart, lung, kedney, brain, platelets, spleen and leukocytes. ADORA2A down-regulates chemokine receptor function and inhibits platelet aggregation. ADORA2A antagonists may be useful as therapy for Parkinson's disease.
M00246
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Description

The adenosine receptors ADORA2A is Gs-coupled GPCRs expressed in the thymus gland, heart, lung, kedney, brain, platelets, spleen and leukocytes. ADORA2A down-regulates chemokine receptor function and inhibits platelet aggregation. ADORA2A antagonists may be useful as therapy for Parkinson's disease.
Synonyms

A2A receptor, A2a, ADORA2, RDC8, A2ar, Adenosine A2a-receptor, A2AAR, AA2AR, Rs

Overview
Applications Functional assay for ADORA2A receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12 K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/ADORA2A/Gα15 Stable Cell Line
    • CHO-K1/ADORA2A/Gα15 Stable Cell Line

    Figure 1. NECA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/ADORA2A/Gα15 cells. The cells were stimulated with agonist NECA and the intracellular cAMP change was measured by Pherastar. The ration of fluorescence signals were normalized and plotted against the log of the cumulative doses of NECA (Mean ± SD, n = 2). The EC50 of NECA on this cell was 2.0 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.