安定細胞株製品 » Human Recombinant Apelin Receptor Stable Cell Line
CHO-K1/AGTRL1/Gα15 Stable Cell Line

Figure 1. Apelin-13-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/AGTRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an AGTRL1 receptor agonist, Apelin-13.The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Apelin-13 (Mean ± SD, n = 2). The EC50 of Apelin-13 on this cells was 0.19 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/AGTRL1/Gα15 Stable Cell Line

CHO-K1/AGTRL1/Gα15 Stable Cell Line

The apelin receptor AGTRL1 is Gi/Go-coupled GPCRs expressed in the heart, coronary artery, aorta, internal mammary artery, pulmonary artery and saphenous vein, it also expressed in lung, kidney and adrenal gland. In AGTRL1 receptor knockout mice baseline blood pressure is not changed compared to wild-type animals. Vasoconstrictor responses to angiotensin II however are significantly more pronounced in the knockout animals.
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Description

The apelin receptor AGTRL1 is Gi/Go-coupled GPCRs expressed in the heart, coronary artery, aorta, internal mammary artery, pulmonary artery and saphenous vein, it also expressed in lung, kidney and adrenal gland. In AGTRL1 receptor knockout mice baseline blood pressure is not changed compared to wild-type animals. Vasoconstrictor responses to angiotensin II however are significantly more pronounced in the knockout animals.
Synonyms

APJ; APJ (apelin) receptor; APJ receptor; APJR; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11; HG11 orphan receptor; angiotensin II receptor-like 1; angiotensin receptor-like 1; apelin receptor

Overview
Applications Functional assay for AGTRL1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12 K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/AGTRL1/Gα15 Stable Cell Line
    • CHO-K1/AGTRL1/Gα15 Stable Cell Line

    Figure 1. Apelin-13-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/AGTRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an AGTRL1 receptor agonist, Apelin-13.The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Apelin-13 (Mean ± SD, n = 2). The EC50 of Apelin-13 on this cells was 0.19 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/AGTRL1/Gα15 Stable Cell Line
    • CHO-K1/AGTRL1/Gα15 Stable Cell Line