安定細胞株製品 » Human Recombinant Neuropeptide Y Receptor Y4 Stable Cell Line
CHO-K1/NPY4/Gα15 Stable Cell Line

Figure 1: Pancreatic Polypeptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPY4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with a NPY4 receptor agonist, pancreatic polypeptide. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of pancreatic polypeptide (Mean ± SD, n = 2). The EC50 of pancreatic polypeptide on this cell was 91 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/NPY4/Gα15 Stable Cell Line

The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. Y4 receptor preferentially binds PP, with significant binding to NPY and PYY.
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Description

The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. Y4 receptor preferentially binds PP, with significant binding to NPY and PYY.
Synonyms

Y4 receptor, PP1, NPY4R, Y4, neuropeptide Y receptor type 4, pancreatic polypeptide receptor 1, NYYR-D, PPYR1

Overview
Applications Functional assay for NPY4 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s) (Cat. #11765, Life Technologies), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/NPY4/Gα15 Stable Cell Line
    • CHO-K1/NPY4/Gα15 Stable Cell Line

    Figure 1: Pancreatic Polypeptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPY4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with a NPY4 receptor agonist, pancreatic polypeptide. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of pancreatic polypeptide (Mean ± SD, n = 2). The EC50 of pancreatic polypeptide on this cell was 91 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to Background Ratio (S/B) = Top/Bottom