安定細胞株製品 » Human Recombinant ADRA1A Adrenoceptors Stable Cell Line
CHO-K1/ADRA1A Stable Cell Line

Figure 1: Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHOK1/ADRA1A cells. The cells were loaded with Calcium-4 prior to being stimulated with an ADRA1A receptor agonist, epinephrine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of epinephrine (Mean ± SD, n = 2). The EC50 of epinephrine on this cell was 0.72 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADRA1A Stable Cell Line

The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D) that mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains predicted to have seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase both inositol 1,4,5-trisphosphate production and intracellular Ca2+. Once activated by binding, α1-ARs initiate the cellular pathways leading to the regulation of physiological effects, including blood pressure maintenance, glucose metabolism, renal sodium reabsorption, and cardiac inotropy.
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Description

The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D) that mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains predicted to have seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase both inositol 1,4,5-trisphosphate production and intracellular Ca2+. Once activated by binding, α1-ARs initiate the cellular pathways leading to the regulation of physiological effects, including blood pressure maintenance, glucose metabolism, renal sodium reabsorption, and cardiac inotropy.
Synonyms

Alpha-1A adrenoceptor, alpha-1A adrenergic receptor

Overview
Applications Functional assay for ADRA1A receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F12 K (Kaighn’s), 10% FBS, 400 µg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/ADRA1A Stable Cell Line
    • CHO-K1/ADRA1A Stable Cell Line

    Figure 1: Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHOK1/ADRA1A cells. The cells were loaded with Calcium-4 prior to being stimulated with an ADRA1A receptor agonist, epinephrine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of epinephrine (Mean ± SD, n = 2). The EC50 of epinephrine on this cell was 0.72 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.