安定細胞株製品 » Human Recombinant NTS1 Neurotensin Receptor Stable Cell Line
CHO-K1/NTS1 Stable Cell Line

Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC50 of neurotensin on this cell was 7.4 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/NTS1 Stable Cell Line

Neurotensin receptor 1 (NTS1) is a member of G-protein coupled receptor family A and is a receptor for Neurotensin (NT). Neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. The NTS1 is expressed in CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system, and esophageal smooth muscle.
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Description

Neurotensin receptor 1 (NTS1) is a member of G-protein coupled receptor family A and is a receptor for Neurotensin (NT). Neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. The NTS1 is expressed in CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system, and esophageal smooth muscle.
Synonyms

NTRH, NTR1, NTSR1, Levocabastin-insensitive neurotensin receptor, High-affinity neurotensin receptor, NTR-1, NT1, NTR, LOC366274, Ntsr, Ntsr1_predicted, ntsr_predicted, NT-R-1, high-affinity levocabastine-insensitive neurotensin receptor, neurotensin receptor, neurotensin receptor 1, neurotensin receptor type 1, NT-1R

Overview
Applications Functional assay for NTS1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/NTS1 Stable Cell Line
    • CHO-K1/NTS1 Stable Cell Line

    Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC50 of neurotensin on this cell was 7.4 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to Background Ratio (S/B) = Top/Bottom