安定細胞株製品 » Human Recombinant BB3 Bombesin Receptor Stable Cell Line
CHO-K1/BB3 Stable Cell Line

Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/BB3 Stable Cell Line

Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/BB3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human bombesin receptor subtype 3 (BB3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00183
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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human bombesin receptor subtype 3 (BB3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Gibco)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/BB3 Stable Cell Line
    • CHO-K1/BB3 Stable Cell Line

    Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/BB3 Stable Cell Line
    • CHO-K1/BB3 Stable Cell Line

    Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB3 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB3 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 3). The EC50 of Neuromedin B on this cell was 0.33 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.