| M00154 | |
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| ¥1,613,997.00 | |
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| Ask us a question | |
| Product Description |
Human Recombinant CHO-K1/CCKB Stable Cell Line
stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring
the In Vitro function of CCKB. |
| Applications |
Functional assay for CCKB receptor (Calcium Flux) |
| Expressed Gene |
Codon optimized from
NM_176875; no expressed tags |
| Target Protein |
NP_795344.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (1×106 per
vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and
recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma
test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant changes in
assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. A5669701,
Gibco),
10% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01,
Life Technologies) |
| Gene Synonyms |
CCK-2R; CCK-B; CCK2R; GASR;
CCKBR |
| Background |
Cholecystokinins (CCK) are derived from preprocholecystokinin.
They are widely expressed in gastrointestinal tissues and in the CNS. There are
two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). CCKBR
can be activated by the smaller CCK-4. In the CNS, CCKBR has been implicated in
anxiety, depression, schizophrenia, depression, and opioid analgesia. In the
stomach, CCK-2 mediates gastrin-stimulated gastric acid secretion. |
For research use only. Not intended for human or animal
clinical trials, therapeutic or diagnostic use.
