安定細胞株製品 » Human Recombinant D2 Dopamine Receptor Stable Cell Line
CHO-K1/D2/Gα15 Stable Cell Line

Figure 2. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of [3H]spiperone in the absence (total binding) or presence of 1000-fold excess unlabeled haloperidol (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

CHO-K1/D2/Gα15 Stable Cell Line

Figure 3. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of haloperidol in the presence of 1nM [3H]haloperidol. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/D2/Gα15 Stable Cell Line

Figure 1: Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, dopamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of dopamine (Mean ± SD, n = 2). The EC50 of dopamine on D2 expressing CHO-K1/Gα15 cells was 18.8 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/D2/Gα15 Stable Cell Line

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). Dopamine receptor 2 is mainly expressed in the brain. It has splicing variants, D2L and D2S. D2R receptor is implicated in a number of neurological and psychiatric conditions. Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson's disease, schizophrenia, and depression.
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Description

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). Dopamine receptor 2 is mainly expressed in the brain. It has splicing variants, D2L and D2S. D2R receptor is implicated in a number of neurological and psychiatric conditions. Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson's disease, schizophrenia, and depression.
Synonyms

D2 receptor, D2A and D2B, D2(415) and D2(444), dopamine D2 receptor, D(2) dopamine receptor, dopamine receptor 2, dopamine receptor D2, Drd-2, D2 receptor, D2R

Overview
Applications Functional assays for D2 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/D2/Gα15 Stable Cell Line
    • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 2. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of [3H]spiperone in the absence (total binding) or presence of 1000-fold excess unlabeled haloperidol (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

  • CHO-K1/D2/Gα15 Stable Cell Line
    • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 3. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of haloperidol in the presence of 1nM [3H]haloperidol. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

  • CHO-K1/D2/Gα15 Stable Cell Line
    • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 1: Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, dopamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of dopamine (Mean ± SD, n = 2). The EC50 of dopamine on D2 expressing CHO-K1/Gα15 cells was 18.8 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.