
Figure 1. Endothelin 1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist endothelin 1. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of endothelin 1 (Mean ± SEM, n = 3). The EC50 of endothelin 1 on this cell was 43.44 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/EDNRB Stable Cell Line
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