Protein Isolation and Analysis » Streptavidin-HRP

Streptavidin-HRP

Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.
M00091
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Overview
Product Description Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin. 
Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.

Properties
Formulation Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal
Reconstitution Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml.
Storage The product remains stable in lyophilized form for at least 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

Applications
Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time, etc. The following dilution ranges are just a guideline:
Application Recommended Usage
Dot blot 1:2,000~1:10,000
Western blot 1:2,000~1:20,000
ELISA 1:5,000~1:20,000
Others User optimized

For research use only. Not intended for human and animal therapeutic or diagnostic use.