| L00295 | |
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| ¥14,241.00 | |
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| Ask us a question | |
| Product Description | The GenScript Ni-Charged MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO4. The Ni-Charged MagBeads have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry). |
| Key Features |
● Quick and convenient separation accomplished by magnetic force ● High binding capacity with 40 mg of 6xHis-tagged protein/ml settled beads ● Compatible with various reagents needed in the purification process ● Can be regenerated for multiple uses ● Extremely low nonspecific binding |
| Ligand | Nickel |
| Binding Capacity | 40 mg 6×His-tagged protein per 1 mL settled beads |
| Matrix | super paramagnetic beads |
| Average Particle Size | 40 µm |
| Formulation | 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO4 |
| Application | small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions |
| Storage | Stored at 2-8°C. Do NOT freeze the product. Keep the magnetic beads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination. |
| Note | This product contains 20 % EtOH as a preservative. Flammable liquid and vapor. Flash point 38°C. |
For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
