UBE4A cDNA ORF clone, Zonotrichia albicollis(white-throated sparrow)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	102060586
Full Name	ubiquitination factor E4A
Gene Type	protein-coding
Organism	Zonotrichia albicollis(white-throated sparrow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014272474.1	XP_014127949.1	ubiquitin conjugation factor E4 A
XM_014272474.2	XP_014127949.1	ubiquitin conjugation factor E4 A

Pathways from BioSystems
Gene Ontology

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZo16600	XM_014272474.2 Latest version!	Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OZo16600	XM_014272474.1	Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( XM_014272474.1 ) cDNA ORF clone, Zonotrichia albicollis(white-throated sparrow) -> XP_014127949.1 Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.

Price & Availability↑

CloneID	OZo16600
Clone ID Related Accession (Same CDS sequence)	XM_014272474.1 , XM_014272474.2
Accession Version	XM_014272474.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Zonotrichia albicollis(white-throated sparrow)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005081781.1) and transcript sequences (GBBB01096450.1 and GBBD01089716.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zonotrichia albicollis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 299 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCGTCGCTG CCGCCAAGCA CTTCGCCGCC ATCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGAGG AGACGTCCAT CAGCCAGGAT GACTCCGACA ACAGTGTCTC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCCGAGATC AGCCGCTCGT TCCGCTCGCA GCGAGAGCTC 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATCT TCCTCATCAC CCTGGACAAC 
AGTGACCCCA GCATGAAGAG TGGGAATGGG ATCCCAGCAC GCTGTGTGTA CCTGGAGGAG 
ATGGCTGCAG ACCTGGATGA CCAGGACTGG CTGGACATGG ACAATGTGGA GCAGGCCCTG 
TTCACCCGCC TGCTGCTGCC GGAGCCCGGG AGCCACCTGA TCCACATGAC CTGTGCCAGC 
AGCCAGAACC TGTCGGCCGA CCGCGACGCC GGGGAGAGGC ACATCCTGCG CTACCTCTAC 
GCCTGCTTCC GCAGGGCCCG CGAGGAGATC ACCAAGGTCC CGGAGAACCT GCTGCCCTTT 
GCCGTGCGCT GCCGCAACCT GACGGTGTCC AACACGCGCA CCGTGCTCCT CACCCCCGAG 
ATCTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGCTGCTGGA GACACTGAGG 
GGGGCACAGT TTGAAGACAT GACCGAGTTC CTCGAGGAGG TCATAGAGGC CTTAACAATG 
GACGAGGAGG TGCGCACATT TGGGGAGGTG ATGGTTCCCG TGTTTGACAT TCTGCTGGGG 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTCTACA CATACCTCGA TGTGCTCCTG 
TACTTCACAA AGCAGAAGGA CATAGCCAAG GTTTTTGCAG GCTACATACA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTTGGTG CCATTCTAAA TATCTCCTGC 
TTGTTGAAGA CCCCTGGCGT GGTGGAGAAC CATGGCTACT TTCTGAATCC ATCCCGATCC 
AGCCCACAGG AGATTAAAGT GCAGGAATCC AACATCCATC AGTTCATGGC CCAGTTCCAT 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGCTGT CACCGGAGAC GAAGCACAGG 
ATCCTGTCCT GGCTGGGGAA CTGCCTCCAT GCGAATGCGG GCCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATTTT CTTCCAGATG TACGCCTCAG ATGCCTTCTT CCTCAACCTG 
GGGGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CCCGATCTCC AAAGCTGCTA 
ACCTTCAACC CTACCTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGTTTGA TCCCTGCTCT GAGCGAGCAA 
GAGCCGCAGT TTGCAAACAG CTACAACCTG GTGACGGAAA ACCTGGTGCT CACGCAGTAT 
ACCCTGCACT TGGGGTTTCA CAGGTTGCAC GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTGGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCCGC TGCTGACAGC 
CTCAGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTGT CCACCAAGAC GGCCATGACG 
GAACCACAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT GCTGCTGGTG 
CAGCTGGCAG TGGGGAACCA GGGCAGCGAG CCGCTGGAGC TGAGCTTCCC GCTGCCGCAG 
GTGGAGCACA GCGCCTTGGC CTACGTGCCA GAGTTCTTTG CCGATAATTT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCTTGGAGA CTTCTGCTGA TTCCCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCTGA AGTGTTGGAG GCTGTGATGC CTCACTTGGA TCAGGCTCAG 
AGCCCGCTCG TCTCGAGCGT GTTCCATCGC AAGCGAGTGT TCTGCTCCTA CCAACACGCC 
GCCCACCTGG CCGAGGCACT CATCAAAGTC TTCGTGGATA TCGAGTTCAC GGGTGACCCA 
CACCAGTTTG AACAGAAGTT CAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGACACTG ATTCCTACCG GGACAGCATA AAGGCTCTGG CTGATTATGC CTCAGAGAAC 
CTGGAGGCAA TGAATCCCCC TCTCTTCTTG CGCTTCCTTA ACCTGCTCAT GAACGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTTCA GCAGATTGAG 
AAGGACCGAG GCGAGTGGGA CAGCCTGTCC CCCGAGGCTC GCCGCGAGAA GGAGTCCAGC 
CTGCAGATGT TTGGTCAGCT GGCCCGTTTC CACAACATCA TGTCCAATGA AACCATTGGC 
ACTCTGGCTT TCCTGACTTC AGAAATCAAG TCCCTGTTTG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACATCTGG TTGGGCCTAA AATGGGAGCT 
TTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAGCTCGT GTCTGACATC 
TGCACCATCT ACCTAAACCT TGGGGATGAA GAAAACTTCT GTGCCACAGT CCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC GCTCTTTGCC CAGACCGTGC GGGTTCTGAA GAAGATTAAC 
AAGCCTGGCA ACATGATTGT GTCTTTTAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGGCAGC AGCAGGAAGA GGAGACATAC GCAGATGCCT GTGATGAATT CCTGGATCCA 
ATCATGAGCA CTCTGATGTC AGACCCCGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGATCGACCA TAGCCCGACA CCTCCTCAGT GATCAGACAG ATCCTTTCAA TCGGAGTCCC 
CTAACTATGG ACCAGATCAG ACCAAACACA GAGCTAAAAG AGAAGATACA GAGATGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGGAG CTGGAGGACA CTCTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014127949.1
CDS	104..3334
Translation	MTDQENNNSISSNPFAALFGSVAAAKHFAAIQKQQQLRQLTGEETSISQDDSDNSVSESLDDCDYSVAEISRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPARCVYLEEMAADLDDQDWLDMDNVEQALFTRLLLPEPGSHLIHMTCASSQNLSADRDAGERHILRYLYACFRRAREEITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVYEQLVDLLLETLRGAQFEDMTEFLEEVIEALTMDEEVRTFGEVMVPVFDILLGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMAQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSPKLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPALSEQEPQFANSYNLVTENLVLTQYTLHLGFHRLHDQMVKINQSLHRLQVAWREAQQSSSPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAVGNQGSEPLELSFPLPQVEHSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHILHFVTVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQSPLVSSVFHRKRVFCSYQHAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWDTDSYRDSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSPEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKKQKEEELEDTLN

Target ORF information:

RefSeq Version	XM_014272474.1
Organism	Zonotrichia albicollis(white-throated sparrow)
Definition	Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014272474.1

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCGTCGCTG CCGCCAAGCA CTTCGCCGCC ATCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGAGG AGACGTCCAT CAGCCAGGAT GACTCCGACA ACAGTGTCTC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCCGAGATC AGCCGCTCGT TCCGCTCGCA GCGAGAGCTC 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATCT TCCTCATCAC CCTGGACAAC 
AGTGACCCCA GCATGAAGAG TGGGAATGGG ATCCCAGCAC GCTGTGTGTA CCTGGAGGAG 
ATGGCTGCAG ACCTGGATGA CCAGGACTGG CTGGACATGG ACAATGTGGA GCAGGCCCTG 
TTCACCCGCC TGCTGCTGCC GGAGCCCGGG AGCCACCTGA TCCACATGAC CTGTGCCAGC 
AGCCAGAACC TGTCGGCCGA CCGCGACGCC GGGGAGAGGC ACATCCTGCG CTACCTCTAC 
GCCTGCTTCC GCAGGGCCCG CGAGGAGATC ACCAAGGTCC CGGAGAACCT GCTGCCCTTT 
GCCGTGCGCT GCCGCAACCT GACGGTGTCC AACACGCGCA CCGTGCTCCT CACCCCCGAG 
ATCTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGCTGCTGGA GACACTGAGG 
GGGGCACAGT TTGAAGACAT GACCGAGTTC CTCGAGGAGG TCATAGAGGC CTTAACAATG 
GACGAGGAGG TGCGCACATT TGGGGAGGTG ATGGTTCCCG TGTTTGACAT TCTGCTGGGG 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTCTACA CATACCTCGA TGTGCTCCTG 
TACTTCACAA AGCAGAAGGA CATAGCCAAG GTTTTTGCAG GCTACATACA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTTGGTG CCATTCTAAA TATCTCCTGC 
TTGTTGAAGA CCCCTGGCGT GGTGGAGAAC CATGGCTACT TTCTGAATCC ATCCCGATCC 
AGCCCACAGG AGATTAAAGT GCAGGAATCC AACATCCATC AGTTCATGGC CCAGTTCCAT 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGCTGT CACCGGAGAC GAAGCACAGG 
ATCCTGTCCT GGCTGGGGAA CTGCCTCCAT GCGAATGCGG GCCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATTTT CTTCCAGATG TACGCCTCAG ATGCCTTCTT CCTCAACCTG 
GGGGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CCCGATCTCC AAAGCTGCTA 
ACCTTCAACC CTACCTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGTTTGA TCCCTGCTCT GAGCGAGCAA 
GAGCCGCAGT TTGCAAACAG CTACAACCTG GTGACGGAAA ACCTGGTGCT CACGCAGTAT 
ACCCTGCACT TGGGGTTTCA CAGGTTGCAC GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTGGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCCGC TGCTGACAGC 
CTCAGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTGT CCACCAAGAC GGCCATGACG 
GAACCACAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT GCTGCTGGTG 
CAGCTGGCAG TGGGGAACCA GGGCAGCGAG CCGCTGGAGC TGAGCTTCCC GCTGCCGCAG 
GTGGAGCACA GCGCCTTGGC CTACGTGCCA GAGTTCTTTG CCGATAATTT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCTTGGAGA CTTCTGCTGA TTCCCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCTGA AGTGTTGGAG GCTGTGATGC CTCACTTGGA TCAGGCTCAG 
AGCCCGCTCG TCTCGAGCGT GTTCCATCGC AAGCGAGTGT TCTGCTCCTA CCAACACGCC 
GCCCACCTGG CCGAGGCACT CATCAAAGTC TTCGTGGATA TCGAGTTCAC GGGTGACCCA 
CACCAGTTTG AACAGAAGTT CAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGACACTG ATTCCTACCG GGACAGCATA AAGGCTCTGG CTGATTATGC CTCAGAGAAC 
CTGGAGGCAA TGAATCCCCC TCTCTTCTTG CGCTTCCTTA ACCTGCTCAT GAACGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTTCA GCAGATTGAG 
AAGGACCGAG GCGAGTGGGA CAGCCTGTCC CCCGAGGCTC GCCGCGAGAA GGAGTCCAGC 
CTGCAGATGT TTGGTCAGCT GGCCCGTTTC CACAACATCA TGTCCAATGA AACCATTGGC 
ACTCTGGCTT TCCTGACTTC AGAAATCAAG TCCCTGTTTG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACATCTGG TTGGGCCTAA AATGGGAGCT 
TTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAGCTCGT GTCTGACATC 
TGCACCATCT ACCTAAACCT TGGGGATGAA GAAAACTTCT GTGCCACAGT CCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC GCTCTTTGCC CAGACCGTGC GGGTTCTGAA GAAGATTAAC 
AAGCCTGGCA ACATGATTGT GTCTTTTAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGGCAGC AGCAGGAAGA GGAGACATAC GCAGATGCCT GTGATGAATT CCTGGATCCA 
ATCATGAGCA CTCTGATGTC AGACCCCGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGATCGACCA TAGCCCGACA CCTCCTCAGT GATCAGACAG ATCCTTTCAA TCGGAGTCCC 
CTAACTATGG ACCAGATCAG ACCAAACACA GAGCTAAAAG AGAAGATACA GAGATGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGGAG CTGGAGGACA CTCTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( XM_014272474.2 ) cDNA ORF clone, Zonotrichia albicollis(white-throated sparrow) -> XP_014127949.1 Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.

Price & Availability↑

CloneID	OZo16600
Clone ID Related Accession (Same CDS sequence)	XM_014272474.1 , XM_014272474.2
Accession Version	XM_014272474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-14
Organism	Zonotrichia albicollis(white-throated sparrow)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005081781.1) and transcript sequences (GBBB01096450.1 and GBBD01089716.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 15, 2018 this sequence version replaced XM_014272474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zonotrichia albicollis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 299 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCGTCGCTG CCGCCAAGCA CTTCGCCGCC ATCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGAGG AGACGTCCAT CAGCCAGGAT GACTCCGACA ACAGTGTCTC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCCGAGATC AGCCGCTCGT TCCGCTCGCA GCGAGAGCTC 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATCT TCCTCATCAC CCTGGACAAC 
AGTGACCCCA GCATGAAGAG TGGGAATGGG ATCCCAGCAC GCTGTGTGTA CCTGGAGGAG 
ATGGCTGCAG ACCTGGATGA CCAGGACTGG CTGGACATGG ACAATGTGGA GCAGGCCCTG 
TTCACCCGCC TGCTGCTGCC GGAGCCCGGG AGCCACCTGA TCCACATGAC CTGTGCCAGC 
AGCCAGAACC TGTCGGCCGA CCGCGACGCC GGGGAGAGGC ACATCCTGCG CTACCTCTAC 
GCCTGCTTCC GCAGGGCCCG CGAGGAGATC ACCAAGGTCC CGGAGAACCT GCTGCCCTTT 
GCCGTGCGCT GCCGCAACCT GACGGTGTCC AACACGCGCA CCGTGCTCCT CACCCCCGAG 
ATCTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGCTGCTGGA GACACTGAGG 
GGGGCACAGT TTGAAGACAT GACCGAGTTC CTCGAGGAGG TCATAGAGGC CTTAACAATG 
GACGAGGAGG TGCGCACATT TGGGGAGGTG ATGGTTCCCG TGTTTGACAT TCTGCTGGGG 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTCTACA CATACCTCGA TGTGCTCCTG 
TACTTCACAA AGCAGAAGGA CATAGCCAAG GTTTTTGCAG GCTACATACA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTTGGTG CCATTCTAAA TATCTCCTGC 
TTGTTGAAGA CCCCTGGCGT GGTGGAGAAC CATGGCTACT TTCTGAATCC ATCCCGATCC 
AGCCCACAGG AGATTAAAGT GCAGGAATCC AACATCCATC AGTTCATGGC CCAGTTCCAT 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGCTGT CACCGGAGAC GAAGCACAGG 
ATCCTGTCCT GGCTGGGGAA CTGCCTCCAT GCGAATGCGG GCCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATTTT CTTCCAGATG TACGCCTCAG ATGCCTTCTT CCTCAACCTG 
GGGGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CCCGATCTCC AAAGCTGCTA 
ACCTTCAACC CTACCTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGTTTGA TCCCTGCTCT GAGCGAGCAA 
GAGCCGCAGT TTGCAAACAG CTACAACCTG GTGACGGAAA ACCTGGTGCT CACGCAGTAT 
ACCCTGCACT TGGGGTTTCA CAGGTTGCAC GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTGGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCCGC TGCTGACAGC 
CTCAGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTGT CCACCAAGAC GGCCATGACG 
GAACCACAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT GCTGCTGGTG 
CAGCTGGCAG TGGGGAACCA GGGCAGCGAG CCGCTGGAGC TGAGCTTCCC GCTGCCGCAG 
GTGGAGCACA GCGCCTTGGC CTACGTGCCA GAGTTCTTTG CCGATAATTT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCTTGGAGA CTTCTGCTGA TTCCCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCTGA AGTGTTGGAG GCTGTGATGC CTCACTTGGA TCAGGCTCAG 
AGCCCGCTCG TCTCGAGCGT GTTCCATCGC AAGCGAGTGT TCTGCTCCTA CCAACACGCC 
GCCCACCTGG CCGAGGCACT CATCAAAGTC TTCGTGGATA TCGAGTTCAC GGGTGACCCA 
CACCAGTTTG AACAGAAGTT CAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGACACTG ATTCCTACCG GGACAGCATA AAGGCTCTGG CTGATTATGC CTCAGAGAAC 
CTGGAGGCAA TGAATCCCCC TCTCTTCTTG CGCTTCCTTA ACCTGCTCAT GAACGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTTCA GCAGATTGAG 
AAGGACCGAG GCGAGTGGGA CAGCCTGTCC CCCGAGGCTC GCCGCGAGAA GGAGTCCAGC 
CTGCAGATGT TTGGTCAGCT GGCCCGTTTC CACAACATCA TGTCCAATGA AACCATTGGC 
ACTCTGGCTT TCCTGACTTC AGAAATCAAG TCCCTGTTTG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACATCTGG TTGGGCCTAA AATGGGAGCT 
TTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAGCTCGT GTCTGACATC 
TGCACCATCT ACCTAAACCT TGGGGATGAA GAAAACTTCT GTGCCACAGT CCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC GCTCTTTGCC CAGACCGTGC GGGTTCTGAA GAAGATTAAC 
AAGCCTGGCA ACATGATTGT GTCTTTTAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGGCAGC AGCAGGAAGA GGAGACATAC GCAGATGCCT GTGATGAATT CCTGGATCCA 
ATCATGAGCA CTCTGATGTC AGACCCCGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGATCGACCA TAGCCCGACA CCTCCTCAGT GATCAGACAG ATCCTTTCAA TCGGAGTCCC 
CTAACTATGG ACCAGATCAG ACCAAACACA GAGCTAAAAG AGAAGATACA GAGATGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGGAG CTGGAGGACA CTCTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014127949.1
CDS	104..3334
Translation	MTDQENNNSISSNPFAALFGSVAAAKHFAAIQKQQQLRQLTGEETSISQDDSDNSVSESLDDCDYSVAEISRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPARCVYLEEMAADLDDQDWLDMDNVEQALFTRLLLPEPGSHLIHMTCASSQNLSADRDAGERHILRYLYACFRRAREEITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVYEQLVDLLLETLRGAQFEDMTEFLEEVIEALTMDEEVRTFGEVMVPVFDILLGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMAQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSPKLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPALSEQEPQFANSYNLVTENLVLTQYTLHLGFHRLHDQMVKINQSLHRLQVAWREAQQSSSPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAVGNQGSEPLELSFPLPQVEHSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHILHFVTVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQSPLVSSVFHRKRVFCSYQHAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWDTDSYRDSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSPEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKKQKEEELEDTLN

Target ORF information:

RefSeq Version	XM_014272474.2
Organism	Zonotrichia albicollis(white-throated sparrow)
Definition	Zonotrichia albicollis ubiquitination factor E4A (UBE4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014272474.2

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCGTCGCTG CCGCCAAGCA CTTCGCCGCC ATCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGAGG AGACGTCCAT CAGCCAGGAT GACTCCGACA ACAGTGTCTC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCCGAGATC AGCCGCTCGT TCCGCTCGCA GCGAGAGCTC 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATCT TCCTCATCAC CCTGGACAAC 
AGTGACCCCA GCATGAAGAG TGGGAATGGG ATCCCAGCAC GCTGTGTGTA CCTGGAGGAG 
ATGGCTGCAG ACCTGGATGA CCAGGACTGG CTGGACATGG ACAATGTGGA GCAGGCCCTG 
TTCACCCGCC TGCTGCTGCC GGAGCCCGGG AGCCACCTGA TCCACATGAC CTGTGCCAGC 
AGCCAGAACC TGTCGGCCGA CCGCGACGCC GGGGAGAGGC ACATCCTGCG CTACCTCTAC 
GCCTGCTTCC GCAGGGCCCG CGAGGAGATC ACCAAGGTCC CGGAGAACCT GCTGCCCTTT 
GCCGTGCGCT GCCGCAACCT GACGGTGTCC AACACGCGCA CCGTGCTCCT CACCCCCGAG 
ATCTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGCTGCTGGA GACACTGAGG 
GGGGCACAGT TTGAAGACAT GACCGAGTTC CTCGAGGAGG TCATAGAGGC CTTAACAATG 
GACGAGGAGG TGCGCACATT TGGGGAGGTG ATGGTTCCCG TGTTTGACAT TCTGCTGGGG 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTCTACA CATACCTCGA TGTGCTCCTG 
TACTTCACAA AGCAGAAGGA CATAGCCAAG GTTTTTGCAG GCTACATACA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTTGGTG CCATTCTAAA TATCTCCTGC 
TTGTTGAAGA CCCCTGGCGT GGTGGAGAAC CATGGCTACT TTCTGAATCC ATCCCGATCC 
AGCCCACAGG AGATTAAAGT GCAGGAATCC AACATCCATC AGTTCATGGC CCAGTTCCAT 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGCTGT CACCGGAGAC GAAGCACAGG 
ATCCTGTCCT GGCTGGGGAA CTGCCTCCAT GCGAATGCGG GCCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATTTT CTTCCAGATG TACGCCTCAG ATGCCTTCTT CCTCAACCTG 
GGGGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CCCGATCTCC AAAGCTGCTA 
ACCTTCAACC CTACCTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGTTTGA TCCCTGCTCT GAGCGAGCAA 
GAGCCGCAGT TTGCAAACAG CTACAACCTG GTGACGGAAA ACCTGGTGCT CACGCAGTAT 
ACCCTGCACT TGGGGTTTCA CAGGTTGCAC GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTGGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCCGC TGCTGACAGC 
CTCAGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTGT CCACCAAGAC GGCCATGACG 
GAACCACAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT GCTGCTGGTG 
CAGCTGGCAG TGGGGAACCA GGGCAGCGAG CCGCTGGAGC TGAGCTTCCC GCTGCCGCAG 
GTGGAGCACA GCGCCTTGGC CTACGTGCCA GAGTTCTTTG CCGATAATTT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCTTGGAGA CTTCTGCTGA TTCCCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCTGA AGTGTTGGAG GCTGTGATGC CTCACTTGGA TCAGGCTCAG 
AGCCCGCTCG TCTCGAGCGT GTTCCATCGC AAGCGAGTGT TCTGCTCCTA CCAACACGCC 
GCCCACCTGG CCGAGGCACT CATCAAAGTC TTCGTGGATA TCGAGTTCAC GGGTGACCCA 
CACCAGTTTG AACAGAAGTT CAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGACACTG ATTCCTACCG GGACAGCATA AAGGCTCTGG CTGATTATGC CTCAGAGAAC 
CTGGAGGCAA TGAATCCCCC TCTCTTCTTG CGCTTCCTTA ACCTGCTCAT GAACGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTTCA GCAGATTGAG 
AAGGACCGAG GCGAGTGGGA CAGCCTGTCC CCCGAGGCTC GCCGCGAGAA GGAGTCCAGC 
CTGCAGATGT TTGGTCAGCT GGCCCGTTTC CACAACATCA TGTCCAATGA AACCATTGGC 
ACTCTGGCTT TCCTGACTTC AGAAATCAAG TCCCTGTTTG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACATCTGG TTGGGCCTAA AATGGGAGCT 
TTGAAGGTGA AAGATTTCAG TGAGTTTGAC TTCAAGCCAC AGCAGCTCGT GTCTGACATC 
TGCACCATCT ACCTAAACCT TGGGGATGAA GAAAACTTCT GTGCCACAGT CCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC GCTCTTTGCC CAGACCGTGC GGGTTCTGAA GAAGATTAAC 
AAGCCTGGCA ACATGATTGT GTCTTTTAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGGCAGC AGCAGGAAGA GGAGACATAC GCAGATGCCT GTGATGAATT CCTGGATCCA 
ATCATGAGCA CTCTGATGTC AGACCCCGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGATCGACCA TAGCCCGACA CCTCCTCAGT GATCAGACAG ATCCTTTCAA TCGGAGTCCC 
CTAACTATGG ACCAGATCAG ACCAAACACA GAGCTAAAAG AGAAGATACA GAGATGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGGAG CTGGAGGACA CTCTGAACTA A