LOC105217978 gene cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

mRNA	Protein	Name
XM_011193274.1	XP_011191576.1	uncharacterized protein LOC105217978 isoform X2
XM_011193275.1	XP_011191577.1	uncharacterized protein LOC105217978 isoform X3
XM_011193276.1	XP_011191578.1	uncharacterized protein LOC105217978 isoform X4
XM_029043874.1	XP_028899707.1	uncharacterized protein LOC105217978 isoform X3
XM_029043877.1	XP_028899710.1	uncharacterized protein LOC105217978 isoform X6
XM_011193273.1	XP_011191575.1	uncharacterized protein LOC105217978 isoform X1
XM_029043869.1	XP_028899702.1	uncharacterized protein LOC105217978 isoform X1
XM_029043870.1	XP_028899703.1	uncharacterized protein LOC105217978 isoform X1
XM_029043871.1	XP_028899704.1	uncharacterized protein LOC105217978 isoform X1
XM_029043872.1	XP_028899705.1	uncharacterized protein LOC105217978 isoform X1
XM_011193273.2	XP_011191575.1	uncharacterized protein LOC105217978 isoform X2
XM_029043873.1	XP_028899706.1	uncharacterized protein LOC105217978 isoform X2
XM_029043876.1	XP_028899709.1	uncharacterized protein LOC105217978 isoform X5
XM_029043875.1	XP_028899708.1	uncharacterized protein LOC105217978 isoform X4
XM_011193276.2	XP_011191578.2	uncharacterized protein LOC105217978 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa205058	XM_011193273.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa205058	XM_029043873.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa205059	XM_011193274.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa205060	XM_011193275.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa205061	XM_011193276.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa257541	XM_029043872.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa257541	XM_029043871.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa257541	XM_029043869.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa257541	XM_029043870.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa257542	XM_029043874.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa257543	XM_029043875.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa257544	XM_029043876.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa257545	XM_029043877.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa257546	XM_011193276.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OBa205058	XM_011193273.1	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

LOC105217978 ( XM_011193273.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191575.1 Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa205058
Clone ID Related Accession (Same CDS sequence)	XM_011193273.1 , XM_011193273.2 , XM_029043873.1
Accession Version	XM_011193273.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191575.1
CDS	41..4006
Translation	MSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193273.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193273.1

ORF Insert Sequence:

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_011193273.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191575.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa205058
Clone ID Related Accession (Same CDS sequence)	XM_011193273.1 , XM_011193273.2 , XM_029043873.1
Accession Version	XM_011193273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011193273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191575.1
CDS	288..4253
Translation	MSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193273.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193273.2

ORF Insert Sequence:

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043873.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899706.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X6, mRNA.

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CloneID	OBa205058
Clone ID Related Accession (Same CDS sequence)	XM_011193273.1 , XM_011193273.2 , XM_029043873.1
Accession Version	XM_029043873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899706.1
CDS	152..4117
Translation	MSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043873.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043873.1

ORF Insert Sequence:

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGCTGGATCA TAATAACATA 
CCTGATAGAG GAATTGCTCA ATGCATGTAT AATTTGCACA AGGATTGGTG GGACTCCAAA 
TTACCGGAAG AGAGCATTCT CTGTTGTTTG AGCGTAATAC CAAAGGTTTT ATTGGCTGGT 
GCCATAACAT TAATGGATGA GGCATATTAT AAATTGGCTG TCTGGCTAAA TGATCACGAA 
AACTATCGCC TTCAGTCCAA ATACGAAAAT CACCTTATTG CAAAAGTGGC ACTTTTTCAA 
TTCGTCAACT CATTTCTGTC CTTGTTCTAT ATCGCTTTCT ATCTCCGAGA TGAGGATAAA 
TTGAAAGAGC AATTGGCCGG TTTGTTAATT TCACGTCAAA TAATTGGTAA CCTACGTGAA 
TCGGCAATAC CTTACTTTAT TGAACAATGG AAACTAGCTA AATTGAGCTT TAACATGTGG 
GGAGCACTAA GCCCTACGCA AGACATCAAT CGAACCATAA TGGATTCCGG TGTTTCAACG 
ACCACATCAA CAGCATCCAC TACCACGGAA AAAGTCAAAG TGGATACACC GCAACAGACG 
CCGACAAAGC ATGTCGCCAG TAGTCCATTG GTAAGAACCA CAACTTCATC TAATAAGCGG 
AATATCGGTC AAGCAGAGAT CGAAAGTACA CTTTACAAGT ACGATGGTAC ATTTTCCGAT 
CATTTGGAGA TGTTGGTGCA GATGGGTTAT GTAGTACTGT TCTCTGCTGC TTTTCCACTG 
GCCGGTATAT GTGCACTCGT CAACAACTTG ATGGAGATAC GCTCGGATGC CTTTAAGCTG 
GCTCACGTTC ATCAAAGACC TTTCGGGCAG CGTGTAGCTA ATATTGGCAC TTGGCAAAAC 
GCGTTTAGTA TTCTGTCACT GGCTGCCGTC ATTGTAAACT GTGCGTTGAT TGGCTTATCA 
GGACAAGTTT CGCGCCTATG GCCAGGTCTA ACAACTGCTC AAACCATAAT TTTGATTGTC 
ACTTTGGAGC ATATTATGTT GGGATTGCGT TCAGCGCTTA CATGGCTGCT GCCCGAATTG 
CCATCATGGT TGGCTGCTGA AATCGCACGC GCCGAACATT GTCGCAGAGA GATGCAATGC 
AAGGGCACTT CGCCGCGGCC CACACCACCG ACACCGCCAT CGACTACATC AACGCAACCA 
GACCAGGATG ACTTAATTTT AGAAGCCACC ACAACGAATG ATCAATCAAT GGAGAGTCAA 
GTTGCTGAGG ATATTTTATA TCAAGAACAA CTGCGTCAAA GCTCTAACTA TCCGCAAATG 
GATGAGATAG TCAATTTCGG TCTACAACGT AATATCGAAG CAGATGTGAA TATATTCGAG 
AATCGTGATT CCTCGCCAGA CTCACCGCCA TCACAAACCA GTACGATACT GATAAGACCT 
CTACATGAAT CAACACCACC AAATAGTGGT TCATCAGTGA CGAGCTTAAT TCAGGATGCT 
GGCCATGGAC ACGGCAGCGC ATGTAAAAGT TGTAAGCTGA AAATGAAAAA AGCGGAAATA 
CCCCCATTTC ATGGATATTC AGTTCGTAAT TTAAGCTCAA TTCAAGCTAA TCAAAGATTG 
CACGCTTTTT CAGGAGCTTC TCTGAGCAGT GCAAGCACCC TGGCATTAAA TACTATCCGC 
CAGCCCATAC ATATACCAGA AATACCGCCT TTCCGGAAAA AATCCAGTGA CTCAGCTGAT 
CATACTGGTA GCAGTGCGAG CAGTAGTCCG CAGCGCTCCA GACATCCGCT TATTTCAACA 
CCTCCACCAT CCATGTTACA GCATCAACCC ACAAAAATAC CGGAGATACC ACCTTATAAA 
CAACGCAAAA TCTCGGCTGA AAGTGCCACA CATATACATA TGAGTGATAA GTTTATGCTC 
AAATCACACG CACCCGAGTG GATGTCCCGC TTGAAAGTCA GCGATAATAT AAACTTGCAT 
AGGAGCATAG ATTGTATTGC AAAGGAGTTA CAAAGTAATA CAGACACAGC GGACATCTTA 
AAGCCAGCAC CATCCTGGAG TAATGTAGCT ATAAAATCGC CTACACAACA GCAAGTGGCT 
GCCTCCTCAT CATCCTCATC CGCTGGTGGC GGCAGTTCGG GCAGTGCTGG CGGCATGGGA 
GTAAGTGTCG GCATTGTTGG GCGTGCTGTA GACAGTGGCC GACCTAGTGT GGGTGGTATT 
TCTACTACGT CGACCTCATC GATGCAACTG AAAAAACTAA CTGACAATGT CAAGGATACA 
ACATCAGCAG AGATAGTCCC AGTAAAAGGG AGTTCTGGTG GTGCAGGCGC AGGAAGTACA 
ACACAAAGTG GCAGCACAGA CGATGAAGCT AAAGCAGCGG AGATTGCAGC GAAAAAATCG 
CGATTAAAGC AAAAGCTTGT GAAAAGCGCG CGATCAGTTG GCATATTCTC GCTCAAACTG 
AAAGAGCGAC GACAGCGCGA GGCGGAGAAG GCGGCAACTA TTGCCATTGA ACATGCCAAG 
GCTTTGGCTA AATTACCACC CCTGCCGCAA CCAGGCGGTG AGCTGTCTCT CATACCTATT 
GAACAGCTAA TACAAATTGA AGATATAATA CCGACACCGA AACCACAACC CACCACATCG 
TCGGCAACAA CGATGTCAGC GGCGGCAAGT TTGTCCACTG CTGTGACGTC ACCACAAGCA 
ATGACACCCT CATCGAGCGC ACTGGCGGCC GCAATGCAGC AATTTCAAAG TTCGGGAAAT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_011193274.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191576.1 Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa205059
Clone ID Related Accession (Same CDS sequence)	XM_011193274.1
Accession Version	XM_011193274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGTGGGACTC CAAATTACCG 
GAAGAGAGCA TTCTCTGTTG TTTGAGCGTA ATACCAAAGG TTTTATTGGC TGGTGCCATA 
ACATTAATGG ATGAGGCATA TTATAAATTG GCTGTCTGGC TAAATGATCA CGAAAACTAT 
CGCCTTCAGT CCAAATACGA AAATCACCTT ATTGCAAAAG TGGCACTTTT TCAATTCGTC 
AACTCATTTC TGTCCTTGTT CTATATCGCT TTCTATCTCC GAGATGAGGA TAAATTGAAA 
GAGCAATTGG CCGGTTTGTT AATTTCACGT CAAATAATTG GTAACCTACG TGAATCGGCA 
ATACCTTACT TTATTGAACA ATGGAAACTA GCTAAATTGA GCTTTAACAT GTGGGGAGCA 
CTAAGCCCTA CGCAAGACAT CAATCGAACC ATAATGGATT CCGGTGTTTC AACGACCACA 
TCAACAGCAT CCACTACCAC GGAAAAAGTC AAAGTGGATA CACCGCAACA GACGCCGACA 
AAGCATGTCG CCAGTAGTCC ATTGGTAAGA ACCACAACTT CATCTAATAA GCGGAATATC 
GGTCAAGCAG AGATCGAAAG TACACTTTAC AAGTACGATG GTACATTTTC CGATCATTTG 
GAGATGTTGG TGCAGATGGG TTATGTAGTA CTGTTCTCTG CTGCTTTTCC ACTGGCCGGT 
ATATGTGCAC TCGTCAACAA CTTGATGGAG ATACGCTCGG ATGCCTTTAA GCTGGCTCAC 
GTTCATCAAA GACCTTTCGG GCAGCGTGTA GCTAATATTG GCACTTGGCA AAACGCGTTT 
AGTATTCTGT CACTGGCTGC CGTCATTGTA AACTGTGCGT TGATTGGCTT ATCAGGACAA 
GTTTCGCGCC TATGGCCAGG TCTAACAACT GCTCAAACCA TAATTTTGAT TGTCACTTTG 
GAGCATATTA TGTTGGGATT GCGTTCAGCG CTTACATGGC TGCTGCCCGA ATTGCCATCA 
TGGTTGGCTG CTGAAATCGC ACGCGCCGAA CATTGTCGCA GAGAGATGCA ATGCAAGGGC 
ACTTCGCCGC GGCCCACACC ACCGACACCG CCATCGACTA CATCAACGCA ACCAGACCAG 
GATGACTTAA TTTTAGAAGC CACCACAACG AATGATCAAT CAATGGAGAG TCAAGTTGCT 
GAGGATATTT TATATCAAGA ACAACTGCGT CAAAGCTCTA ACTATCCGCA AATGGATGAG 
ATAGTCAATT TCGGTCTACA ACGTAATATC GAAGCAGATG TGAATATATT CGAGAATCGT 
GATTCCTCGC CAGACTCACC GCCATCACAA ACCAGTACGA TACTGATAAG ACCTCTACAT 
GAATCAACAC CACCAAATAG TGGTTCATCA GTGACGAGCT TAATTCAGGA TGCTGGCCAT 
GGACACGGCA GCGCATGTAA AAGTTGTAAG CTGAAAATGA AAAAAGCGGA AATACCCCCA 
TTTCATGGAT ATTCAGTTCG TAATTTAAGC TCAATTCAAG CTAATCAAAG ATTGCACGCT 
TTTTCAGGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG ATAAGTTTAT GCTCAAATCA 
CACGCACCCG AGTGGATGTC CCGCTTGAAA GTCAGCGATA ATATAAACTT GCATAGGAGC 
ATAGATTGTA TTGCAAAGGA GTTACAAAGT AATACAGACA CAGCGGACAT CTTAAAGCCA 
GCACCATCCT GGAGTAATGT AGCTATAAAA TCGCCTACAC AACAGCAAGT GGCTGCCTCC 
TCATCATCCT CATCCGCTGG TGGCGGCAGT TCGGGCAGTG CTGGCGGCAT GGGAGTAAGT 
GTCGGCATTG TTGGGCGTGC TGTAGACAGT GGCCGACCTA GTGTGGGTGG TATTTCTACT 
ACGTCGACCT CATCGATGCA ACTGAAAAAA CTAACTGACA ATGTCAAGGA TACAACATCA 
GCAGAGATAG TCCCAGTAAA AGGGAGTTCT GGTGGTGCAG GCGCAGGAAG TACAACACAA 
AGTGGCAGCA CAGACGATGA AGCTAAAGCA GCGGAGATTG CAGCGAAAAA ATCGCGATTA 
AAGCAAAAGC TTGTGAAAAG CGCGCGATCA GTTGGCATAT TCTCGCTCAA ACTGAAAGAG 
CGACGACAGC GCGAGGCGGA GAAGGCGGCA ACTATTGCCA TTGAACATGC CAAGGCTTTG 
GCTAAATTAC CACCCCTGCC GCAACCAGGC GGTGAGCTGT CTCTCATACC TATTGAACAG 
CTAATACAAA TTGAAGATAT AATACCGACA CCGAAACCAC AACCCACCAC ATCGTCGGCA 
ACAACGATGT CAGCGGCGGC AAGTTTGTCC ACTGCTGTGA CGTCACCACA AGCAATGACA 
CCCTCATCGA GCGCACTGGC GGCCGCAATG CAGCAATTTC AAAGTTCGGG AAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191576.1
CDS	41..3940
Translation	MSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193274.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193274.1

ORF Insert Sequence:

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGTGGGACTC CAAATTACCG 
GAAGAGAGCA TTCTCTGTTG TTTGAGCGTA ATACCAAAGG TTTTATTGGC TGGTGCCATA 
ACATTAATGG ATGAGGCATA TTATAAATTG GCTGTCTGGC TAAATGATCA CGAAAACTAT 
CGCCTTCAGT CCAAATACGA AAATCACCTT ATTGCAAAAG TGGCACTTTT TCAATTCGTC 
AACTCATTTC TGTCCTTGTT CTATATCGCT TTCTATCTCC GAGATGAGGA TAAATTGAAA 
GAGCAATTGG CCGGTTTGTT AATTTCACGT CAAATAATTG GTAACCTACG TGAATCGGCA 
ATACCTTACT TTATTGAACA ATGGAAACTA GCTAAATTGA GCTTTAACAT GTGGGGAGCA 
CTAAGCCCTA CGCAAGACAT CAATCGAACC ATAATGGATT CCGGTGTTTC AACGACCACA 
TCAACAGCAT CCACTACCAC GGAAAAAGTC AAAGTGGATA CACCGCAACA GACGCCGACA 
AAGCATGTCG CCAGTAGTCC ATTGGTAAGA ACCACAACTT CATCTAATAA GCGGAATATC 
GGTCAAGCAG AGATCGAAAG TACACTTTAC AAGTACGATG GTACATTTTC CGATCATTTG 
GAGATGTTGG TGCAGATGGG TTATGTAGTA CTGTTCTCTG CTGCTTTTCC ACTGGCCGGT 
ATATGTGCAC TCGTCAACAA CTTGATGGAG ATACGCTCGG ATGCCTTTAA GCTGGCTCAC 
GTTCATCAAA GACCTTTCGG GCAGCGTGTA GCTAATATTG GCACTTGGCA AAACGCGTTT 
AGTATTCTGT CACTGGCTGC CGTCATTGTA AACTGTGCGT TGATTGGCTT ATCAGGACAA 
GTTTCGCGCC TATGGCCAGG TCTAACAACT GCTCAAACCA TAATTTTGAT TGTCACTTTG 
GAGCATATTA TGTTGGGATT GCGTTCAGCG CTTACATGGC TGCTGCCCGA ATTGCCATCA 
TGGTTGGCTG CTGAAATCGC ACGCGCCGAA CATTGTCGCA GAGAGATGCA ATGCAAGGGC 
ACTTCGCCGC GGCCCACACC ACCGACACCG CCATCGACTA CATCAACGCA ACCAGACCAG 
GATGACTTAA TTTTAGAAGC CACCACAACG AATGATCAAT CAATGGAGAG TCAAGTTGCT 
GAGGATATTT TATATCAAGA ACAACTGCGT CAAAGCTCTA ACTATCCGCA AATGGATGAG 
ATAGTCAATT TCGGTCTACA ACGTAATATC GAAGCAGATG TGAATATATT CGAGAATCGT 
GATTCCTCGC CAGACTCACC GCCATCACAA ACCAGTACGA TACTGATAAG ACCTCTACAT 
GAATCAACAC CACCAAATAG TGGTTCATCA GTGACGAGCT TAATTCAGGA TGCTGGCCAT 
GGACACGGCA GCGCATGTAA AAGTTGTAAG CTGAAAATGA AAAAAGCGGA AATACCCCCA 
TTTCATGGAT ATTCAGTTCG TAATTTAAGC TCAATTCAAG CTAATCAAAG ATTGCACGCT 
TTTTCAGGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG ATAAGTTTAT GCTCAAATCA 
CACGCACCCG AGTGGATGTC CCGCTTGAAA GTCAGCGATA ATATAAACTT GCATAGGAGC 
ATAGATTGTA TTGCAAAGGA GTTACAAAGT AATACAGACA CAGCGGACAT CTTAAAGCCA 
GCACCATCCT GGAGTAATGT AGCTATAAAA TCGCCTACAC AACAGCAAGT GGCTGCCTCC 
TCATCATCCT CATCCGCTGG TGGCGGCAGT TCGGGCAGTG CTGGCGGCAT GGGAGTAAGT 
GTCGGCATTG TTGGGCGTGC TGTAGACAGT GGCCGACCTA GTGTGGGTGG TATTTCTACT 
ACGTCGACCT CATCGATGCA ACTGAAAAAA CTAACTGACA ATGTCAAGGA TACAACATCA 
GCAGAGATAG TCCCAGTAAA AGGGAGTTCT GGTGGTGCAG GCGCAGGAAG TACAACACAA 
AGTGGCAGCA CAGACGATGA AGCTAAAGCA GCGGAGATTG CAGCGAAAAA ATCGCGATTA 
AAGCAAAAGC TTGTGAAAAG CGCGCGATCA GTTGGCATAT TCTCGCTCAA ACTGAAAGAG 
CGACGACAGC GCGAGGCGGA GAAGGCGGCA ACTATTGCCA TTGAACATGC CAAGGCTTTG 
GCTAAATTAC CACCCCTGCC GCAACCAGGC GGTGAGCTGT CTCTCATACC TATTGAACAG 
CTAATACAAA TTGAAGATAT AATACCGACA CCGAAACCAC AACCCACCAC ATCGTCGGCA 
ACAACGATGT CAGCGGCGGC AAGTTTGTCC ACTGCTGTGA CGTCACCACA AGCAATGACA 
CCCTCATCGA GCGCACTGGC GGCCGCAATG CAGCAATTTC AAAGTTCGGG AAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_011193275.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191577.1 Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa205060
Clone ID Related Accession (Same CDS sequence)	XM_011193275.1
Accession Version	XM_011193275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG AGTTACAAAG TAATACAGAC 
ACAGCGGACA TCTTAAAGCC AGCACCATCC TGGAGTAATG TAGCTATAAA ATCGCCTACA 
CAACAGCAAG TGGCTGCCTC CTCATCATCC TCATCCGCTG GTGGCGGCAG TTCGGGCAGT 
GCTGGCGGCA TGGGAGTAAG TGTCGGCATT GTTGGGCGTG CTGTAGACAG TGGCCGACCT 
AGTGTGGGTG GTATTTCTAC TACGTCGACC TCATCGATGC AACTGAAAAA ACTAACTGAC 
AATGTCAAGG ATACAACATC AGCAGAGATA GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA 
GGCGCAGGAA GTACAACACA AAGTGGCAGC ACAGACGATG AAGCTAAAGC AGCGGAGATT 
GCAGCGAAAA AATCGCGATT AAAGCAAAAG CTTGTGAAAA GCGCGCGATC AGTTGGCATA 
TTCTCGCTCA AACTGAAAGA GCGACGACAG CGCGAGGCGG AGAAGGCGGC AACTATTGCC 
ATTGAACATG CCAAGGCTTT GGCTAAATTA CCACCCCTGC CGCAACCAGG CGGTGAGCTG 
TCTCTCATAC CTATTGAACA GCTAATACAA ATTGAAGATA TAATACCGAC ACCGAAACCA 
CAACCCACCA CATCGTCGGC AACAACGATG TCAGCGGCGG CAAGTTTGTC CACTGCTGTG 
ACGTCACCAC AAGCAATGAC ACCCTCATCG AGCGCACTGG CGGCCGCAAT GCAGCAATTT 
CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191577.1
CDS	1..3801
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGRASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193275.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193275.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG AGTTACAAAG TAATACAGAC 
ACAGCGGACA TCTTAAAGCC AGCACCATCC TGGAGTAATG TAGCTATAAA ATCGCCTACA 
CAACAGCAAG TGGCTGCCTC CTCATCATCC TCATCCGCTG GTGGCGGCAG TTCGGGCAGT 
GCTGGCGGCA TGGGAGTAAG TGTCGGCATT GTTGGGCGTG CTGTAGACAG TGGCCGACCT 
AGTGTGGGTG GTATTTCTAC TACGTCGACC TCATCGATGC AACTGAAAAA ACTAACTGAC 
AATGTCAAGG ATACAACATC AGCAGAGATA GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA 
GGCGCAGGAA GTACAACACA AAGTGGCAGC ACAGACGATG AAGCTAAAGC AGCGGAGATT 
GCAGCGAAAA AATCGCGATT AAAGCAAAAG CTTGTGAAAA GCGCGCGATC AGTTGGCATA 
TTCTCGCTCA AACTGAAAGA GCGACGACAG CGCGAGGCGG AGAAGGCGGC AACTATTGCC 
ATTGAACATG CCAAGGCTTT GGCTAAATTA CCACCCCTGC CGCAACCAGG CGGTGAGCTG 
TCTCTCATAC CTATTGAACA GCTAATACAA ATTGAAGATA TAATACCGAC ACCGAAACCA 
CAACCCACCA CATCGTCGGC AACAACGATG TCAGCGGCGG CAAGTTTGTC CACTGCTGTG 
ACGTCACCAC AAGCAATGAC ACCCTCATCG AGCGCACTGG CGGCCGCAAT GCAGCAATTT 
CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_011193276.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191578.1 Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.

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CloneID	OBa205061
Clone ID Related Accession (Same CDS sequence)	XM_011193276.1
Accession Version	XM_011193276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGTGGGACTC CAAATTACCG 
GAAGAGAGCA TTCTCTGTTG TTTGAGCGTA ATACCAAAGG TTTTATTGGC TGGTGCCATA 
ACATTAATGG ATGAGGCATA TTATAAATTG GCTGTCTGGC TAAATGATCA CGAAAACTAT 
CGCCTTCAGT CCAAATACGA AAATCACCTT ATTGCAAAAG TGGCACTTTT TCAATTCGTC 
AACTCATTTC TGTCCTTGTT CTATATCGCT TTCTATCTCC GAGATGAGGA TAAATTGAAA 
GAGCAATTGG CCGGTTTGTT AATTTCACGT CAAATAATTG GTAACCTACG TGAATCGGCA 
ATACCTTACT TTATTGAACA ATGGAAACTA GCTAAATTGA GCTTTAACAT GTGGGGAGCA 
CTAAGCCCTA CGCAAGACAT CAATCGAACC ATAATGGATT CCGGTGTTTC AACGACCACA 
TCAACAGCAT CCACTACCAC GGAAAAAGTC AAAGTGGATA CACCGCAACA GACGCCGACA 
AAGCATGTCG CCAGTAGTCC ATTGGTAAGA ACCACAACTT CATCTAATAA GCGGAATATC 
GGTCAAGCAG AGATCGAAAG TACACTTTAC AAGTACGATG GTACATTTTC CGATCATTTG 
GAGATGTTGG TGCAGATGGG TTATGTAGTA CTGTTCTCTG CTGCTTTTCC ACTGGCCGGT 
ATATGTGCAC TCGTCAACAA CTTGATGGAG ATACGCTCGG ATGCCTTTAA GCTGGCTCAC 
GTTCATCAAA GACCTTTCGG GCAGCGTGTA GCTAATATTG GCACTTGGCA AAACGCGTTT 
AGTATTCTGT CACTGGCTGC CGTCATTGTA AACTGTGCGT TGATTGGCTT ATCAGGACAA 
GTTTCGCGCC TATGGCCAGG TCTAACAACT GCTCAAACCA TAATTTTGAT TGTCACTTTG 
GAGCATATTA TGTTGGGATT GCGTTCAGCG CTTACATGGC TGCTGCCCGA ATTGCCATCA 
TGGTTGGCTG CTGAAATCGC ACGCGCCGAA CATTGTCGCA GAGAGATGCA ATGCAAGGGC 
ACTTCGCCGC GGCCCACACC ACCGACACCG CCATCGACTA CATCAACGCA ACCAGACCAG 
GATGACTTAA TTTTAGAAGC CACCACAACG AATGATCAAT CAATGGAGAG TCAAGTTGCT 
GAGGATATTT TATATCAAGA ACAACTGCGT CAAAGCTCTA ACTATCCGCA AATGGATGAG 
ATAGTCAATT TCGGTCTACA ACGTAATATC GAAGCAGATG TGAATATATT CGAGAATCGT 
GATTCCTCGC CAGACTCACC GCCATCACAA ACCAGTACGA TACTGATAAG ACCTCTACAT 
GAATCAACAC CACCAAATAG TGGTTCATCA GTGACGAGCT TAATTCAGGA TGCTGGCCAT 
GGACACGGCA GCGCATGTAA AAGTTGTAAG CTGAAAATGA AAAAAGCGGA AATACCCCCA 
TTTCATGGAT ATTCAGTTCG TAATTTAAGC TCAATTCAAG CTAATCAAAG ATTGCACGCT 
TTTTCAGGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG AGTTACAAAG TAATACAGAC 
ACAGCGGACA TCTTAAAGCC AGCACCATCC TGGAGTAATG TAGCTATAAA ATCGCCTACA 
CAACAGCAAG TGGCTGCCTC CTCATCATCC TCATCCGCTG GTGGCGGCAG TTCGGGCAGT 
GCTGGCGGCA TGGGAGTAAG TGTCGGCATT GTTGGGCGTG CTGTAGACAG TGGCCGACCT 
AGTGTGGGTG GTATTTCTAC TACGTCGACC TCATCGATGC AACTGAAAAA ACTAACTGAC 
AATGTCAAGG ATACAACATC AGCAGAGATA GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA 
GGCGCAGGAA GTACAACACA AAGTGGCAGC ACAGACGATG AAGCTAAAGC AGCGGAGATT 
GCAGCGAAAA AATCGCGATT AAAGCAAAAG CTTGTGAAAA GCGCGCGATC AGTTGGCATA 
TTCTCGCTCA AACTGAAAGA GCGACGACAG CGCGAGGCGG AGAAGGCGGC AACTATTGCC 
ATTGAACATG CCAAGGCTTT GGCTAAATTA CCACCCCTGC CGCAACCAGG CGGTGAGCTG 
TCTCTCATAC CTATTGAACA GCTAATACAA ATTGAAGATA TAATACCGAC ACCGAAACCA 
CAACCCACCA CATCGTCGGC AACAACGATG TCAGCGGCGG CAAGTTTGTC CACTGCTGTG 
ACGTCACCAC AAGCAATGAC ACCCTCATCG AGCGCACTGG CGGCCGCAAT GCAGCAATTT 
CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191578.1
CDS	41..3841
Translation	MSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193276.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193276.1

ORF Insert Sequence:

ATGTCCTCCG AACGACAATC GTCCGAAGAT GTACACGAAT CGACAGCGGA GGATGCCGGC 
TTGGATGCCG GAGATGACTT TACCGATGAC ACTAACTTCG AATCAAATCT GCGTAGACGC 
AAGGGTAAAA TCATTGCGTG TGCCAAGGAA ACGATTGAAA ATGCGTCCCG TCAATTACGT 
CGAAAAGTAC CATATGCTGG ACATTTAATG ACGCCGCGTC GTCTTTGGTT GAATAAAATA 
CCGACACAAT GTGATGTTGT CATCGAGTTT CCAGAAGATG CGCCTGACGA GGCGCTACGT 
TGGCTTCTCT CCAGATTGCG TTCTCAGCCA CCATCGGGGC TTGGATTGGT CGTACAAGTG 
AAGGCTCATG AGAGTACACG GCGGAATGCT TTCTATATTA GTGCACCAAC GAATGTTTTA 
TTTAAAGCGG CCGAAGAAGC ACGATTACCG AAACGCTTAC GTCCTGACCT AGGTGGTGCC 
TTACGTGAAT TCACAACACG CGAAAGTCAT TGTTTTCAGC AACTGAAAAC TGAAGATGGG 
AGCAGTGCAC TTTTCACTTC ACAAGAGCGT CAATGGCTGG TGCTGCAGGT ATTGCAAGGC 
CTCCGTGCCA GTCAAGCGGA TATTGAGGCC CTACAGGGGC GTGCAACTGT AGCCGAAGGG 
CAAAGCATAG TTGCAGCCTG GCAAGAGACT GGACTGATTG TGCAGGTTTT TCCGCTGCAT 
GAAACCCGCT CGCTGACGCT GCTACAAAGC AGTTGGGTGA AACAGATATT TGCGCCGCAA 
CCGCTTGATG ATATAGCTGA ATATTTTGGT GTGAAGGTGG CGCTTTACTT TGCTTGGCTG 
GGCCATTACA CCTGCGCCTT AGGCGTGCCA GCTGTCTTTG GCACAATATT GTATGCAGCA 
CTCTGGGGAA AAGGACAAAC AGCACAAGAT ATGGGACACG TTTTATTCTC ACTCTTTAAT 
GTAGCGTGGG CGTCATTATA TCTAGAGGCG TGGAAGCGAT ATTCTGTGGA ACTGGCCTTT 
CGTTGGGGTA CCCTATCGAC ACCCCCGGAA CTACTCGAAC CACCTAGACC ACTCTATAAG 
GGTAGTTTAG TAGAAAATAA TGTAACTGGA CGACTTGAAC CAAGGGAAGC GCCCGCGTGG 
AAGCGTCGTG CTTTTCGTTA CCTAGTTAGT TTTCCAGTGA TCGGAATGTG CCTAGCCCTT 
GTTTTTGTGG TTATGTTCCT GATGCTACGA TTCCAGGATT GGTGGGACTC CAAATTACCG 
GAAGAGAGCA TTCTCTGTTG TTTGAGCGTA ATACCAAAGG TTTTATTGGC TGGTGCCATA 
ACATTAATGG ATGAGGCATA TTATAAATTG GCTGTCTGGC TAAATGATCA CGAAAACTAT 
CGCCTTCAGT CCAAATACGA AAATCACCTT ATTGCAAAAG TGGCACTTTT TCAATTCGTC 
AACTCATTTC TGTCCTTGTT CTATATCGCT TTCTATCTCC GAGATGAGGA TAAATTGAAA 
GAGCAATTGG CCGGTTTGTT AATTTCACGT CAAATAATTG GTAACCTACG TGAATCGGCA 
ATACCTTACT TTATTGAACA ATGGAAACTA GCTAAATTGA GCTTTAACAT GTGGGGAGCA 
CTAAGCCCTA CGCAAGACAT CAATCGAACC ATAATGGATT CCGGTGTTTC AACGACCACA 
TCAACAGCAT CCACTACCAC GGAAAAAGTC AAAGTGGATA CACCGCAACA GACGCCGACA 
AAGCATGTCG CCAGTAGTCC ATTGGTAAGA ACCACAACTT CATCTAATAA GCGGAATATC 
GGTCAAGCAG AGATCGAAAG TACACTTTAC AAGTACGATG GTACATTTTC CGATCATTTG 
GAGATGTTGG TGCAGATGGG TTATGTAGTA CTGTTCTCTG CTGCTTTTCC ACTGGCCGGT 
ATATGTGCAC TCGTCAACAA CTTGATGGAG ATACGCTCGG ATGCCTTTAA GCTGGCTCAC 
GTTCATCAAA GACCTTTCGG GCAGCGTGTA GCTAATATTG GCACTTGGCA AAACGCGTTT 
AGTATTCTGT CACTGGCTGC CGTCATTGTA AACTGTGCGT TGATTGGCTT ATCAGGACAA 
GTTTCGCGCC TATGGCCAGG TCTAACAACT GCTCAAACCA TAATTTTGAT TGTCACTTTG 
GAGCATATTA TGTTGGGATT GCGTTCAGCG CTTACATGGC TGCTGCCCGA ATTGCCATCA 
TGGTTGGCTG CTGAAATCGC ACGCGCCGAA CATTGTCGCA GAGAGATGCA ATGCAAGGGC 
ACTTCGCCGC GGCCCACACC ACCGACACCG CCATCGACTA CATCAACGCA ACCAGACCAG 
GATGACTTAA TTTTAGAAGC CACCACAACG AATGATCAAT CAATGGAGAG TCAAGTTGCT 
GAGGATATTT TATATCAAGA ACAACTGCGT CAAAGCTCTA ACTATCCGCA AATGGATGAG 
ATAGTCAATT TCGGTCTACA ACGTAATATC GAAGCAGATG TGAATATATT CGAGAATCGT 
GATTCCTCGC CAGACTCACC GCCATCACAA ACCAGTACGA TACTGATAAG ACCTCTACAT 
GAATCAACAC CACCAAATAG TGGTTCATCA GTGACGAGCT TAATTCAGGA TGCTGGCCAT 
GGACACGGCA GCGCATGTAA AAGTTGTAAG CTGAAAATGA AAAAAGCGGA AATACCCCCA 
TTTCATGGAT ATTCAGTTCG TAATTTAAGC TCAATTCAAG CTAATCAAAG ATTGCACGCT 
TTTTCAGGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG AGTTACAAAG TAATACAGAC 
ACAGCGGACA TCTTAAAGCC AGCACCATCC TGGAGTAATG TAGCTATAAA ATCGCCTACA 
CAACAGCAAG TGGCTGCCTC CTCATCATCC TCATCCGCTG GTGGCGGCAG TTCGGGCAGT 
GCTGGCGGCA TGGGAGTAAG TGTCGGCATT GTTGGGCGTG CTGTAGACAG TGGCCGACCT 
AGTGTGGGTG GTATTTCTAC TACGTCGACC TCATCGATGC AACTGAAAAA ACTAACTGAC 
AATGTCAAGG ATACAACATC AGCAGAGATA GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA 
GGCGCAGGAA GTACAACACA AAGTGGCAGC ACAGACGATG AAGCTAAAGC AGCGGAGATT 
GCAGCGAAAA AATCGCGATT AAAGCAAAAG CTTGTGAAAA GCGCGCGATC AGTTGGCATA 
TTCTCGCTCA AACTGAAAGA GCGACGACAG CGCGAGGCGG AGAAGGCGGC AACTATTGCC 
ATTGAACATG CCAAGGCTTT GGCTAAATTA CCACCCCTGC CGCAACCAGG CGGTGAGCTG 
TCTCTCATAC CTATTGAACA GCTAATACAA ATTGAAGATA TAATACCGAC ACCGAAACCA 
CAACCCACCA CATCGTCGGC AACAACGATG TCAGCGGCGG CAAGTTTGTC CACTGCTGTG 
ACGTCACCAC AAGCAATGAC ACCCTCATCG AGCGCACTGG CGGCCGCAAT GCAGCAATTT 
CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043869.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899702.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.

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CloneID	OBa257541
Clone ID Related Accession (Same CDS sequence)	XM_029043869.1 , XM_029043870.1 , XM_029043871.1 , XM_029043872.1
Accession Version	XM_029043869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899702.1
CDS	448..4464
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043869.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043869.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043870.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899703.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa257541
Clone ID Related Accession (Same CDS sequence)	XM_029043869.1 , XM_029043870.1 , XM_029043871.1 , XM_029043872.1
Accession Version	XM_029043870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899703.1
CDS	443..4459
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043870.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043870.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043871.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899704.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa257541
Clone ID Related Accession (Same CDS sequence)	XM_029043869.1 , XM_029043870.1 , XM_029043871.1 , XM_029043872.1
Accession Version	XM_029043871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899704.1
CDS	350..4366
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043871.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043871.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043872.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899705.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.

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CloneID	OBa257541
Clone ID Related Accession (Same CDS sequence)	XM_029043869.1 , XM_029043870.1 , XM_029043871.1 , XM_029043872.1
Accession Version	XM_029043872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899705.1
CDS	319..4335
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043872.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043872.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGATA AGTTTATGCT CAAATCACAC 
GCACCCGAGT GGATGTCCCG CTTGAAAGTC AGCGATAATA TAAACTTGCA TAGGAGCATA 
GATTGTATTG CAAAGGAGTT ACAAAGTAAT ACAGACACAG CGGACATCTT AAAGCCAGCA 
CCATCCTGGA GTAATGTAGC TATAAAATCG CCTACACAAC AGCAAGTGGC TGCCTCCTCA 
TCATCCTCAT CCGCTGGTGG CGGCAGTTCG GGCAGTGCTG GCGGCATGGG AGTAAGTGTC 
GGCATTGTTG GGCGTGCTGT AGACAGTGGC CGACCTAGTG TGGGTGGTAT TTCTACTACG 
TCGACCTCAT CGATGCAACT GAAAAAACTA ACTGACAATG TCAAGGATAC AACATCAGCA 
GAGATAGTCC CAGTAAAAGG GAGTTCTGGT GGTGCAGGCG CAGGAAGTAC AACACAAAGT 
GGCAGCACAG ACGATGAAGC TAAAGCAGCG GAGATTGCAG CGAAAAAATC GCGATTAAAG 
CAAAAGCTTG TGAAAAGCGC GCGATCAGTT GGCATATTCT CGCTCAAACT GAAAGAGCGA 
CGACAGCGCG AGGCGGAGAA GGCGGCAACT ATTGCCATTG AACATGCCAA GGCTTTGGCT 
AAATTACCAC CCCTGCCGCA ACCAGGCGGT GAGCTGTCTC TCATACCTAT TGAACAGCTA 
ATACAAATTG AAGATATAAT ACCGACACCG AAACCACAAC CCACCACATC GTCGGCAACA 
ACGATGTCAG CGGCGGCAAG TTTGTCCACT GCTGTGACGT CACCACAAGC AATGACACCC 
TCATCGAGCG CACTGGCGGC CGCAATGCAG CAATTTCAAA GTTCGGGAAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043874.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899707.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa257542
Clone ID Related Accession (Same CDS sequence)	XM_029043874.1
Accession Version	XM_029043874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGTGGGACT CCAAATTACC GGAAGAGAGC 
ATTCTCTGTT GTTTGAGCGT AATACCAAAG GTTTTATTGG CTGGTGCCAT AACATTAATG 
GATGAGGCAT ATTATAAATT GGCTGTCTGG CTAAATGATC ACGAAAACTA TCGCCTTCAG 
TCCAAATACG AAAATCACCT TATTGCAAAA GTGGCACTTT TTCAATTCGT CAACTCATTT 
CTGTCCTTGT TCTATATCGC TTTCTATCTC CGAGATGAGG ATAAATTGAA AGAGCAATTG 
GCCGGTTTGT TAATTTCACG TCAAATAATT GGTAACCTAC GTGAATCGGC AATACCTTAC 
TTTATTGAAC AATGGAAACT AGCTAAATTG AGCTTTAACA TGTGGGGAGC ACTAAGCCCT 
ACGCAAGACA TCAATCGAAC CATAATGGAT TCCGGTGTTT CAACGACCAC ATCAACAGCA 
TCCACTACCA CGGAAAAAGT CAAAGTGGAT ACACCGCAAC AGACGCCGAC AAAGCATGTC 
GCCAGTAGTC CATTGGTAAG AACCACAACT TCATCTAATA AGCGGAATAT CGGTCAAGCA 
GAGATCGAAA GTACACTTTA CAAGTACGAT GGTACATTTT CCGATCATTT GGAGATGTTG 
GTGCAGATGG GTTATGTAGT ACTGTTCTCT GCTGCTTTTC CACTGGCCGG TATATGTGCA 
CTCGTCAACA ACTTGATGGA GATACGCTCG GATGCCTTTA AGCTGGCTCA CGTTCATCAA 
AGACCTTTCG GGCAGCGTGT AGCTAATATT GGCACTTGGC AAAACGCGTT TAGTATTCTG 
TCACTGGCTG CCGTCATTGT AAACTGTGCG TTGATTGGCT TATCAGGACA AGTTTCGCGC 
CTATGGCCAG GTCTAACAAC TGCTCAAACC ATAATTTTGA TTGTCACTTT GGAGCATATT 
ATGTTGGGAT TGCGTTCAGC GCTTACATGG CTGCTGCCCG AATTGCCATC ATGGTTGGCT 
GCTGAAATCG CACGCGCCGA ACATTGTCGC AGAGAGATGC AATGCAAGGG CACTTCGCCG 
CGGCCCACAC CACCGACACC GCCATCGACT ACATCAACGC AACCAGACCA GGATGACTTA 
ATTTTAGAAG CCACCACAAC GAATGATCAA TCAATGGAGA GTCAAGTTGC TGAGGATATT 
TTATATCAAG AACAACTGCG TCAAAGCTCT AACTATCCGC AAATGGATGA GATAGTCAAT 
TTCGGTCTAC AACGTAATAT CGAAGCAGAT GTGAATATAT TCGAGAATCG TGATTCCTCG 
CCAGACTCAC CGCCATCACA AACCAGTACG ATACTGATAA GACCTCTACA TGAATCAACA 
CCACCAAATA GTGGTTCATC AGTGACGAGC TTAATTCAGG ATGCTGGCCA TGGACACGGC 
AGCGCATGTA AAAGTTGTAA GCTGAAAATG AAAAAAGCGG AAATACCCCC ATTTCATGGA 
TATTCAGTTC GTAATTTAAG CTCAATTCAA GCTAATCAAA GATTGCACGC TTTTTCAGGA 
GCTTCTCTGA GCAGTGCAAG CACCCTGGCA TTAAATACTA TCCGCCAGCC CATACATATA 
CCAGAAATAC CGCCTTTCCG GAAAAAATCC AGTGACTCAG CTGATCATAC TGGTAGCAGT 
GCGAGCAGTA GTCCGCAGCG CTCCAGACAT CCGCTTATTT CAACACCTCC ACCATCCATG 
TTACAGCATC AACCCACAAA AATACCGGAG ATACCACCTT ATAAACAACG CAAAATCTCG 
GCTGAAAGTG CCACACATAT ACATATGAGT GATAAGTTTA TGCTCAAATC ACACGCACCC 
GAGTGGATGT CCCGCTTGAA AGTCAGCGAT AATATAAACT TGCATAGGAG CATAGATTGT 
ATTGCAAAGG AGTTACAAAG TAATACAGAC ACAGCGGACA TCTTAAAGCC AGCACCATCC 
TGGAGTAATG TAGCTATAAA ATCGCCTACA CAACAGCAAG TGGCTGCCTC CTCATCATCC 
TCATCCGCTG GTGGCGGCAG TTCGGGCAGT GCTGGCGGCA TGGGAGTAAG TGTCGGCATT 
GTTGGGCGTG CTGTAGACAG TGGCCGACCT AGTGTGGGTG GTATTTCTAC TACGTCGACC 
TCATCGATGC AACTGAAAAA ACTAACTGAC AATGTCAAGG ATACAACATC AGCAGAGATA 
GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA GGCGCAGGAA GTACAACACA AAGTGGCAGC 
ACAGACGATG AAGCTAAAGC AGCGGAGATT GCAGCGAAAA AATCGCGATT AAAGCAAAAG 
CTTGTGAAAA GCGCGCGATC AGTTGGCATA TTCTCGCTCA AACTGAAAGA GCGACGACAG 
CGCGAGGCGG AGAAGGCGGC AACTATTGCC ATTGAACATG CCAAGGCTTT GGCTAAATTA 
CCACCCCTGC CGCAACCAGG CGGTGAGCTG TCTCTCATAC CTATTGAACA GCTAATACAA 
ATTGAAGATA TAATACCGAC ACCGAAACCA CAACCCACCA CATCGTCGGC AACAACGATG 
TCAGCGGCGG CAAGTTTGTC CACTGCTGTG ACGTCACCAC AAGCAATGAC ACCCTCATCG 
AGCGCACTGG CGGCCGCAAT GCAGCAATTT CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899707.1
CDS	448..4398
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043874.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043874.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGTGGGACT CCAAATTACC GGAAGAGAGC 
ATTCTCTGTT GTTTGAGCGT AATACCAAAG GTTTTATTGG CTGGTGCCAT AACATTAATG 
GATGAGGCAT ATTATAAATT GGCTGTCTGG CTAAATGATC ACGAAAACTA TCGCCTTCAG 
TCCAAATACG AAAATCACCT TATTGCAAAA GTGGCACTTT TTCAATTCGT CAACTCATTT 
CTGTCCTTGT TCTATATCGC TTTCTATCTC CGAGATGAGG ATAAATTGAA AGAGCAATTG 
GCCGGTTTGT TAATTTCACG TCAAATAATT GGTAACCTAC GTGAATCGGC AATACCTTAC 
TTTATTGAAC AATGGAAACT AGCTAAATTG AGCTTTAACA TGTGGGGAGC ACTAAGCCCT 
ACGCAAGACA TCAATCGAAC CATAATGGAT TCCGGTGTTT CAACGACCAC ATCAACAGCA 
TCCACTACCA CGGAAAAAGT CAAAGTGGAT ACACCGCAAC AGACGCCGAC AAAGCATGTC 
GCCAGTAGTC CATTGGTAAG AACCACAACT TCATCTAATA AGCGGAATAT CGGTCAAGCA 
GAGATCGAAA GTACACTTTA CAAGTACGAT GGTACATTTT CCGATCATTT GGAGATGTTG 
GTGCAGATGG GTTATGTAGT ACTGTTCTCT GCTGCTTTTC CACTGGCCGG TATATGTGCA 
CTCGTCAACA ACTTGATGGA GATACGCTCG GATGCCTTTA AGCTGGCTCA CGTTCATCAA 
AGACCTTTCG GGCAGCGTGT AGCTAATATT GGCACTTGGC AAAACGCGTT TAGTATTCTG 
TCACTGGCTG CCGTCATTGT AAACTGTGCG TTGATTGGCT TATCAGGACA AGTTTCGCGC 
CTATGGCCAG GTCTAACAAC TGCTCAAACC ATAATTTTGA TTGTCACTTT GGAGCATATT 
ATGTTGGGAT TGCGTTCAGC GCTTACATGG CTGCTGCCCG AATTGCCATC ATGGTTGGCT 
GCTGAAATCG CACGCGCCGA ACATTGTCGC AGAGAGATGC AATGCAAGGG CACTTCGCCG 
CGGCCCACAC CACCGACACC GCCATCGACT ACATCAACGC AACCAGACCA GGATGACTTA 
ATTTTAGAAG CCACCACAAC GAATGATCAA TCAATGGAGA GTCAAGTTGC TGAGGATATT 
TTATATCAAG AACAACTGCG TCAAAGCTCT AACTATCCGC AAATGGATGA GATAGTCAAT 
TTCGGTCTAC AACGTAATAT CGAAGCAGAT GTGAATATAT TCGAGAATCG TGATTCCTCG 
CCAGACTCAC CGCCATCACA AACCAGTACG ATACTGATAA GACCTCTACA TGAATCAACA 
CCACCAAATA GTGGTTCATC AGTGACGAGC TTAATTCAGG ATGCTGGCCA TGGACACGGC 
AGCGCATGTA AAAGTTGTAA GCTGAAAATG AAAAAAGCGG AAATACCCCC ATTTCATGGA 
TATTCAGTTC GTAATTTAAG CTCAATTCAA GCTAATCAAA GATTGCACGC TTTTTCAGGA 
GCTTCTCTGA GCAGTGCAAG CACCCTGGCA TTAAATACTA TCCGCCAGCC CATACATATA 
CCAGAAATAC CGCCTTTCCG GAAAAAATCC AGTGACTCAG CTGATCATAC TGGTAGCAGT 
GCGAGCAGTA GTCCGCAGCG CTCCAGACAT CCGCTTATTT CAACACCTCC ACCATCCATG 
TTACAGCATC AACCCACAAA AATACCGGAG ATACCACCTT ATAAACAACG CAAAATCTCG 
GCTGAAAGTG CCACACATAT ACATATGAGT GATAAGTTTA TGCTCAAATC ACACGCACCC 
GAGTGGATGT CCCGCTTGAA AGTCAGCGAT AATATAAACT TGCATAGGAG CATAGATTGT 
ATTGCAAAGG AGTTACAAAG TAATACAGAC ACAGCGGACA TCTTAAAGCC AGCACCATCC 
TGGAGTAATG TAGCTATAAA ATCGCCTACA CAACAGCAAG TGGCTGCCTC CTCATCATCC 
TCATCCGCTG GTGGCGGCAG TTCGGGCAGT GCTGGCGGCA TGGGAGTAAG TGTCGGCATT 
GTTGGGCGTG CTGTAGACAG TGGCCGACCT AGTGTGGGTG GTATTTCTAC TACGTCGACC 
TCATCGATGC AACTGAAAAA ACTAACTGAC AATGTCAAGG ATACAACATC AGCAGAGATA 
GTCCCAGTAA AAGGGAGTTC TGGTGGTGCA GGCGCAGGAA GTACAACACA AAGTGGCAGC 
ACAGACGATG AAGCTAAAGC AGCGGAGATT GCAGCGAAAA AATCGCGATT AAAGCAAAAG 
CTTGTGAAAA GCGCGCGATC AGTTGGCATA TTCTCGCTCA AACTGAAAGA GCGACGACAG 
CGCGAGGCGG AGAAGGCGGC AACTATTGCC ATTGAACATG CCAAGGCTTT GGCTAAATTA 
CCACCCCTGC CGCAACCAGG CGGTGAGCTG TCTCTCATAC CTATTGAACA GCTAATACAA 
ATTGAAGATA TAATACCGAC ACCGAAACCA CAACCCACCA CATCGTCGGC AACAACGATG 
TCAGCGGCGG CAAGTTTGTC CACTGCTGTG ACGTCACCAC AAGCAATGAC ACCCTCATCG 
AGCGCACTGG CGGCCGCAAT GCAGCAATTT CAAAGTTCGG GAAATCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043875.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899708.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa257543
Clone ID Related Accession (Same CDS sequence)	XM_029043875.1
Accession Version	XM_029043875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGAGT TACAAAGTAA TACAGACACA 
GCGGACATCT TAAAGCCAGC ACCATCCTGG AGTAATGTAG CTATAAAATC GCCTACACAA 
CAGCAAGTGG CTGCCTCCTC ATCATCCTCA TCCGCTGGTG GCGGCAGTTC GGGCAGTGCT 
GGCGGCATGG GAGTAAGTGT CGGCATTGTT GGGCGTGCTG TAGACAGTGG CCGACCTAGT 
GTGGGTGGTA TTTCTACTAC GTCGACCTCA TCGATGCAAC TGAAAAAACT AACTGACAAT 
GTCAAGGATA CAACATCAGC AGAGATAGTC CCAGTAAAAG GGAGTTCTGG TGGTGCAGGC 
GCAGGAAGTA CAACACAAAG TGGCAGCACA GACGATGAAG CTAAAGCAGC GGAGATTGCA 
GCGAAAAAAT CGCGATTAAA GCAAAAGCTT GTGAAAAGCG CGCGATCAGT TGGCATATTC 
TCGCTCAAAC TGAAAGAGCG ACGACAGCGC GAGGCGGAGA AGGCGGCAAC TATTGCCATT 
GAACATGCCA AGGCTTTGGC TAAATTACCA CCCCTGCCGC AACCAGGCGG TGAGCTGTCT 
CTCATACCTA TTGAACAGCT AATACAAATT GAAGATATAA TACCGACACC GAAACCACAA 
CCCACCACAT CGTCGGCAAC AACGATGTCA GCGGCGGCAA GTTTGTCCAC TGCTGTGACG 
TCACCACAAG CAATGACACC CTCATCGAGC GCACTGGCGG CCGCAATGCA GCAATTTCAA 
AGTTCGGGAA ATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899708.1
CDS	448..4365
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043875.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043875.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGCG CATGTAAAAG TTGTAAGCTG AAAATGAAAA AAGCGGAAAT ACCCCCATTT 
CATGGATATT CAGTTCGTAA TTTAAGCTCA ATTCAAGCTA ATCAAAGATT GCACGCTTTT 
TCAGGAGCTT CTCTGAGCAG TGCAAGCACC CTGGCATTAA ATACTATCCG CCAGCCCATA 
CATATACCAG AAATACCGCC TTTCCGGAAA AAATCCAGTG ACTCAGCTGA TCATACTGGT 
AGCAGTGCGA GCAGTAGTCC GCAGCGCTCC AGACATCCGC TTATTTCAAC ACCTCCACCA 
TCCATGTTAC AGCATCAACC CACAAAAATA CCGGAGATAC CACCTTATAA ACAACGCAAA 
ATCTCGGCTG AAAGTGCCAC ACATATACAT ATGAGTGAGT TACAAAGTAA TACAGACACA 
GCGGACATCT TAAAGCCAGC ACCATCCTGG AGTAATGTAG CTATAAAATC GCCTACACAA 
CAGCAAGTGG CTGCCTCCTC ATCATCCTCA TCCGCTGGTG GCGGCAGTTC GGGCAGTGCT 
GGCGGCATGG GAGTAAGTGT CGGCATTGTT GGGCGTGCTG TAGACAGTGG CCGACCTAGT 
GTGGGTGGTA TTTCTACTAC GTCGACCTCA TCGATGCAAC TGAAAAAACT AACTGACAAT 
GTCAAGGATA CAACATCAGC AGAGATAGTC CCAGTAAAAG GGAGTTCTGG TGGTGCAGGC 
GCAGGAAGTA CAACACAAAG TGGCAGCACA GACGATGAAG CTAAAGCAGC GGAGATTGCA 
GCGAAAAAAT CGCGATTAAA GCAAAAGCTT GTGAAAAGCG CGCGATCAGT TGGCATATTC 
TCGCTCAAAC TGAAAGAGCG ACGACAGCGC GAGGCGGAGA AGGCGGCAAC TATTGCCATT 
GAACATGCCA AGGCTTTGGC TAAATTACCA CCCCTGCCGC AACCAGGCGG TGAGCTGTCT 
CTCATACCTA TTGAACAGCT AATACAAATT GAAGATATAA TACCGACACC GAAACCACAA 
CCCACCACAT CGTCGGCAAC AACGATGTCA GCGGCGGCAA GTTTGTCCAC TGCTGTGACG 
TCACCACAAG CAATGACACC CTCATCGAGC GCACTGGCGG CCGCAATGCA GCAATTTCAA 
AGTTCGGGAA ATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043876.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899709.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X9, mRNA.

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CloneID	OBa257544
Clone ID Related Accession (Same CDS sequence)	XM_029043876.1
Accession Version	XM_029043876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG ATAAGTTTAT GCTCAAATCA 
CACGCACCCG AGTGGATGTC CCGCTTGAAA GTCAGCGATA ATATAAACTT GCATAGGAGC 
ATAGATTGTA TTGCAAAGGA GTTACAAAGT AATACAGACA CAGCGGACAT CTTAAAGCCA 
GCACCATCCT GGAGTAATGT AGCTATAAAA TCGCCTACAC AACAGCAAGT GGCTGCCTCC 
TCATCATCCT CATCCGCTGG TGGCGGCAGT TCGGGCAGTG CTGGCGGCAT GGGAGTAAGT 
GTCGGCATTG TTGGGCGTGC TGTAGACAGT GGCCGACCTA GTGTGGGTGG TATTTCTACT 
ACGTCGACCT CATCGATGCA ACTGAAAAAA CTAACTGACA ATGTCAAGGA TACAACATCA 
GCAGAGATAG TCCCAGTAAA AGGGAGTTCT GGTGGTGCAG GCGCAGGAAG TACAACACAA 
AGTGGCAGCA CAGACGATGA AGCTAAAGCA GCGGAGATTG CAGCGAAAAA ATCGCGATTA 
AAGCAAAAGC TTGTGAAAAG CGCGCGATCA GTTGGCATAT TCTCGCTCAA ACTGAAAGAG 
CGACGACAGC GCGAGGCGGA GAAGGCGGCA ACTATTGCCA TTGAACATGC CAAGGCTTTG 
GCTAAATTAC CACCCCTGCC GCAACCAGGC GGTGAGCTGT CTCTCATACC TATTGAACAG 
CTAATACAAA TTGAAGATAT AATACCGACA CCGAAACCAC AACCCACCAC ATCGTCGGCA 
ACAACGATGT CAGCGGCGGC AAGTTTGTCC ACTGCTGTGA CGTCACCACA AGCAATGACA 
CCCTCATCGA GCGCACTGGC GGCCGCAATG CAGCAATTTC AAAGTTCGGG AAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899709.1
CDS	448..4347
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGRASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043876.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043876.1

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGCTGGATC ATAATAACAT ACCTGATAGA 
GGAATTGCTC AATGCATGTA TAATTTGCAC AAGGATTGGT GGGACTCCAA ATTACCGGAA 
GAGAGCATTC TCTGTTGTTT GAGCGTAATA CCAAAGGTTT TATTGGCTGG TGCCATAACA 
TTAATGGATG AGGCATATTA TAAATTGGCT GTCTGGCTAA ATGATCACGA AAACTATCGC 
CTTCAGTCCA AATACGAAAA TCACCTTATT GCAAAAGTGG CACTTTTTCA ATTCGTCAAC 
TCATTTCTGT CCTTGTTCTA TATCGCTTTC TATCTCCGAG ATGAGGATAA ATTGAAAGAG 
CAATTGGCCG GTTTGTTAAT TTCACGTCAA ATAATTGGTA ACCTACGTGA ATCGGCAATA 
CCTTACTTTA TTGAACAATG GAAACTAGCT AAATTGAGCT TTAACATGTG GGGAGCACTA 
AGCCCTACGC AAGACATCAA TCGAACCATA ATGGATTCCG GTGTTTCAAC GACCACATCA 
ACAGCATCCA CTACCACGGA AAAAGTCAAA GTGGATACAC CGCAACAGAC GCCGACAAAG 
CATGTCGCCA GTAGTCCATT GGTAAGAACC ACAACTTCAT CTAATAAGCG GAATATCGGT 
CAAGCAGAGA TCGAAAGTAC ACTTTACAAG TACGATGGTA CATTTTCCGA TCATTTGGAG 
ATGTTGGTGC AGATGGGTTA TGTAGTACTG TTCTCTGCTG CTTTTCCACT GGCCGGTATA 
TGTGCACTCG TCAACAACTT GATGGAGATA CGCTCGGATG CCTTTAAGCT GGCTCACGTT 
CATCAAAGAC CTTTCGGGCA GCGTGTAGCT AATATTGGCA CTTGGCAAAA CGCGTTTAGT 
ATTCTGTCAC TGGCTGCCGT CATTGTAAAC TGTGCGTTGA TTGGCTTATC AGGACAAGTT 
TCGCGCCTAT GGCCAGGTCT AACAACTGCT CAAACCATAA TTTTGATTGT CACTTTGGAG 
CATATTATGT TGGGATTGCG TTCAGCGCTT ACATGGCTGC TGCCCGAATT GCCATCATGG 
TTGGCTGCTG AAATCGCACG CGCCGAACAT TGTCGCAGAG AGATGCAATG CAAGGGCACT 
TCGCCGCGGC CCACACCACC GACACCGCCA TCGACTACAT CAACGCAACC AGACCAGGAT 
GACTTAATTT TAGAAGCCAC CACAACGAAT GATCAATCAA TGGAGAGTCA AGTTGCTGAG 
GATATTTTAT ATCAAGAACA ACTGCGTCAA AGCTCTAACT ATCCGCAAAT GGATGAGATA 
GTCAATTTCG GTCTACAACG TAATATCGAA GCAGATGTGA ATATATTCGA GAATCGTGAT 
TCCTCGCCAG ACTCACCGCC ATCACAAACC AGTACGATAC TGATAAGACC TCTACATGAA 
TCAACACCAC CAAATAGTGG TTCATCAGTG ACGAGCTTAA TTCAGGATGC TGGCCATGGA 
CACGGCAGAG CTTCTCTGAG CAGTGCAAGC ACCCTGGCAT TAAATACTAT CCGCCAGCCC 
ATACATATAC CAGAAATACC GCCTTTCCGG AAAAAATCCA GTGACTCAGC TGATCATACT 
GGTAGCAGTG CGAGCAGTAG TCCGCAGCGC TCCAGACATC CGCTTATTTC AACACCTCCA 
CCATCCATGT TACAGCATCA ACCCACAAAA ATACCGGAGA TACCACCTTA TAAACAACGC 
AAAATCTCGG CTGAAAGTGC CACACATATA CATATGAGTG ATAAGTTTAT GCTCAAATCA 
CACGCACCCG AGTGGATGTC CCGCTTGAAA GTCAGCGATA ATATAAACTT GCATAGGAGC 
ATAGATTGTA TTGCAAAGGA GTTACAAAGT AATACAGACA CAGCGGACAT CTTAAAGCCA 
GCACCATCCT GGAGTAATGT AGCTATAAAA TCGCCTACAC AACAGCAAGT GGCTGCCTCC 
TCATCATCCT CATCCGCTGG TGGCGGCAGT TCGGGCAGTG CTGGCGGCAT GGGAGTAAGT 
GTCGGCATTG TTGGGCGTGC TGTAGACAGT GGCCGACCTA GTGTGGGTGG TATTTCTACT 
ACGTCGACCT CATCGATGCA ACTGAAAAAA CTAACTGACA ATGTCAAGGA TACAACATCA 
GCAGAGATAG TCCCAGTAAA AGGGAGTTCT GGTGGTGCAG GCGCAGGAAG TACAACACAA 
AGTGGCAGCA CAGACGATGA AGCTAAAGCA GCGGAGATTG CAGCGAAAAA ATCGCGATTA 
AAGCAAAAGC TTGTGAAAAG CGCGCGATCA GTTGGCATAT TCTCGCTCAA ACTGAAAGAG 
CGACGACAGC GCGAGGCGGA GAAGGCGGCA ACTATTGCCA TTGAACATGC CAAGGCTTTG 
GCTAAATTAC CACCCCTGCC GCAACCAGGC GGTGAGCTGT CTCTCATACC TATTGAACAG 
CTAATACAAA TTGAAGATAT AATACCGACA CCGAAACCAC AACCCACCAC ATCGTCGGCA 
ACAACGATGT CAGCGGCGGC AAGTTTGTCC ACTGCTGTGA CGTCACCACA AGCAATGACA 
CCCTCATCGA GCGCACTGGC GGCCGCAATG CAGCAATTTC AAAGTTCGGG AAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_029043877.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899710.1 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa257545
Clone ID Related Accession (Same CDS sequence)	XM_029043877.1
Accession Version	XM_029043877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCCGC GTCGTCTTTG GTTGAATAAA ATACCGACAC AATGTGATGT TGTCATCGAG 
TTTCCAGAAG ATGCGCCTGA CGAGGCGCTA CGTTGGCTTC TCTCCAGATT GCGTTCTCAG 
CCACCATCGG GGCTTGGATT GGTCGTACAA GTGAAGGCTC ATGAGAGTAC ACGGCGGAAT 
GCTTTCTATA TTAGTGCACC AACGAATGTT TTATTTAAAG CGGCCGAAGA AGCACGATTA 
CCGAAACGCT TACGTCCTGA CCTAGGTGGT GCCTTACGTG AATTCACAAC ACGCGAAAGT 
CATTGTTTTC AGCAACTGAA AACTGAAGAT GGGAGCAGTG CACTTTTCAC TTCACAAGAG 
CGTCAATGGC TGGTGCTGCA GGTATTGCAA GGCCTCCGTG CCAGTCAAGC GGATATTGAG 
GCCCTACAGG GGCGTGCAAC TGTAGCCGAA GGGCAAAGCA TAGTTGCAGC CTGGCAAGAG 
ACTGGACTGA TTGTGCAGGT TTTTCCGCTG CATGAAACCC GCTCGCTGAC GCTGCTACAA 
AGCAGTTGGG TGAAACAGAT ATTTGCGCCG CAACCGCTTG ATGATATAGC TGAATATTTT 
GGTGTGAAGG TGGCGCTTTA CTTTGCTTGG CTGGGCCATT ACACCTGCGC CTTAGGCGTG 
CCAGCTGTCT TTGGCACAAT ATTGTATGCA GCACTCTGGG GAAAAGGACA AACAGCACAA 
GATATGGGAC ACGTTTTATT CTCACTCTTT AATGTAGCGT GGGCGTCATT ATATCTAGAG 
GCGTGGAAGC GATATTCTGT GGAACTGGCC TTTCGTTGGG GTACCCTATC GACACCCCCG 
GAACTACTCG AACCACCTAG ACCACTCTAT AAGGGTAGTT TAGTAGAAAA TAATGTAACT 
GGACGACTTG AACCAAGGGA AGCGCCCGCG TGGAAGCGTC GTGCTTTTCG TTACCTAGTT 
AGTTTTCCAG TGATCGGAAT GTGCCTAGCC CTTGTTTTTG TGGTTATGTT CCTGATGCTA 
CGATTCCAGG ATTGGCTGGA TCATAATAAC ATACCTGATA GAGGAATTGC TCAATGCATG 
TATAATTTGC ACAAGGATTG GTGGGACTCC AAATTACCGG AAGAGAGCAT TCTCTGTTGT 
TTGAGCGTAA TACCAAAGGT TTTATTGGCT GGTGCCATAA CATTAATGGA TGAGGCATAT 
TATAAATTGG CTGTCTGGCT AAATGATCAC GAAAACTATC GCCTTCAGTC CAAATACGAA 
AATCACCTTA TTGCAAAAGT GGCACTTTTT CAATTCGTCA ACTCATTTCT GTCCTTGTTC 
TATATCGCTT TCTATCTCCG AGATGAGGAT AAATTGAAAG AGCAATTGGC CGGTTTGTTA 
ATTTCACGTC AAATAATTGG TAACCTACGT GAATCGGCAA TACCTTACTT TATTGAACAA 
TGGAAACTAG CTAAATTGAG CTTTAACATG TGGGGAGCAC TAAGCCCTAC GCAAGACATC 
AATCGAACCA TAATGGATTC CGGTGTTTCA ACGACCACAT CAACAGCATC CACTACCACG 
GAAAAAGTCA AAGTGGATAC ACCGCAACAG ACGCCGACAA AGCATGTCGC CAGTAGTCCA 
TTGGTAAGAA CCACAACTTC ATCTAATAAG CGGAATATCG GTCAAGCAGA GATCGAAAGT 
ACACTTTACA AGTACGATGG TACATTTTCC GATCATTTGG AGATGTTGGT GCAGATGGGT 
TATGTAGTAC TGTTCTCTGC TGCTTTTCCA CTGGCCGGTA TATGTGCACT CGTCAACAAC 
TTGATGGAGA TACGCTCGGA TGCCTTTAAG CTGGCTCACG TTCATCAAAG ACCTTTCGGG 
CAGCGTGTAG CTAATATTGG CACTTGGCAA AACGCGTTTA GTATTCTGTC ACTGGCTGCC 
GTCATTGTAA ACTGTGCGTT GATTGGCTTA TCAGGACAAG TTTCGCGCCT ATGGCCAGGT 
CTAACAACTG CTCAAACCAT AATTTTGATT GTCACTTTGG AGCATATTAT GTTGGGATTG 
CGTTCAGCGC TTACATGGCT GCTGCCCGAA TTGCCATCAT GGTTGGCTGC TGAAATCGCA 
CGCGCCGAAC ATTGTCGCAG AGAGATGCAA TGCAAGGGCA CTTCGCCGCG GCCCACACCA 
CCGACACCGC CATCGACTAC ATCAACGCAA CCAGACCAGG ATGACTTAAT TTTAGAAGCC 
ACCACAACGA ATGATCAATC AATGGAGAGT CAAGTTGCTG AGGATATTTT ATATCAAGAA 
CAACTGCGTC AAAGCTCTAA CTATCCGCAA ATGGATGAGA TAGTCAATTT CGGTCTACAA 
CGTAATATCG AAGCAGATGT GAATATATTC GAGAATCGTG ATTCCTCGCC AGACTCACCG 
CCATCACAAA CCAGTACGAT ACTGATAAGA CCTCTACATG AATCAACACC ACCAAATAGT 
GGTTCATCAG TGACGAGCTT AATTCAGGAT GCTGGCCATG GACACGGCAG CGCATGTAAA 
AGTTGTAAGC TGAAAATGAA AAAAGCGGAA ATACCCCCAT TTCATGGATA TTCAGTTCGT 
AATTTAAGCT CAATTCAAGC TAATCAAAGA TTGCACGCTT TTTCAGGAGC TTCTCTGAGC 
AGTGCAAGCA CCCTGGCATT AAATACTATC CGCCAGCCCA TACATATACC AGAAATACCG 
CCTTTCCGGA AAAAATCCAG TGACTCAGCT GATCATACTG GTAGCAGTGC GAGCAGTAGT 
CCGCAGCGCT CCAGACATCC GCTTATTTCA ACACCTCCAC CATCCATGTT ACAGCATCAA 
CCCACAAAAA TACCGGAGAT ACCACCTTAT AAACAACGCA AAATCTCGGC TGAAAGTGCC 
ACACATATAC ATATGAGTGA TAAGTTTATG CTCAAATCAC ACGCACCCGA GTGGATGTCC 
CGCTTGAAAG TCAGCGATAA TATAAACTTG CATAGGAGCA TAGATTGTAT TGCAAAGGAG 
TTACAAAGTA ATACAGACAC AGCGGACATC TTAAAGCCAG CACCATCCTG GAGTAATGTA 
GCTATAAAAT CGCCTACACA ACAGCAAGTG GCTGCCTCCT CATCATCCTC ATCCGCTGGT 
GGCGGCAGTT CGGGCAGTGC TGGCGGCATG GGAGTAAGTG TCGGCATTGT TGGGCGTGCT 
GTAGACAGTG GCCGACCTAG TGTGGGTGGT ATTTCTACTA CGTCGACCTC ATCGATGCAA 
CTGAAAAAAC TAACTGACAA TGTCAAGGAT ACAACATCAG CAGAGATAGT CCCAGTAAAA 
GGGAGTTCTG GTGGTGCAGG CGCAGGAAGT ACAACACAAA GTGGCAGCAC AGACGATGAA 
GCTAAAGCAG CGGAGATTGC AGCGAAAAAA TCGCGATTAA AGCAAAAGCT TGTGAAAAGC 
GCGCGATCAG TTGGCATATT CTCGCTCAAA CTGAAAGAGC GACGACAGCG CGAGGCGGAG 
AAGGCGGCAA CTATTGCCAT TGAACATGCC AAGGCTTTGG CTAAATTACC ACCCCTGCCG 
CAACCAGGCG GTGAGCTGTC TCTCATACCT ATTGAACAGC TAATACAAAT TGAAGATATA 
ATACCGACAC CGAAACCACA ACCCACCACA TCGTCGGCAA CAACGATGTC AGCGGCGGCA 
AGTTTGTCCA CTGCTGTGAC GTCACCACAA GCAATGACAC CCTCATCGAG CGCACTGGCG 
GCCGCAATGC AGCAATTTCA AAGTTCGGGA AATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899710.1
CDS	292..4050
Translation	MTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWLDHNNIPDRGIAQCMYNLHKDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSDKFMLKSHAPEWMSRLKVSDNINLHRSIDCIAKELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_029043877.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043877.1

ORF Insert Sequence:

ATGACGCCGC GTCGTCTTTG GTTGAATAAA ATACCGACAC AATGTGATGT TGTCATCGAG 
TTTCCAGAAG ATGCGCCTGA CGAGGCGCTA CGTTGGCTTC TCTCCAGATT GCGTTCTCAG 
CCACCATCGG GGCTTGGATT GGTCGTACAA GTGAAGGCTC ATGAGAGTAC ACGGCGGAAT 
GCTTTCTATA TTAGTGCACC AACGAATGTT TTATTTAAAG CGGCCGAAGA AGCACGATTA 
CCGAAACGCT TACGTCCTGA CCTAGGTGGT GCCTTACGTG AATTCACAAC ACGCGAAAGT 
CATTGTTTTC AGCAACTGAA AACTGAAGAT GGGAGCAGTG CACTTTTCAC TTCACAAGAG 
CGTCAATGGC TGGTGCTGCA GGTATTGCAA GGCCTCCGTG CCAGTCAAGC GGATATTGAG 
GCCCTACAGG GGCGTGCAAC TGTAGCCGAA GGGCAAAGCA TAGTTGCAGC CTGGCAAGAG 
ACTGGACTGA TTGTGCAGGT TTTTCCGCTG CATGAAACCC GCTCGCTGAC GCTGCTACAA 
AGCAGTTGGG TGAAACAGAT ATTTGCGCCG CAACCGCTTG ATGATATAGC TGAATATTTT 
GGTGTGAAGG TGGCGCTTTA CTTTGCTTGG CTGGGCCATT ACACCTGCGC CTTAGGCGTG 
CCAGCTGTCT TTGGCACAAT ATTGTATGCA GCACTCTGGG GAAAAGGACA AACAGCACAA 
GATATGGGAC ACGTTTTATT CTCACTCTTT AATGTAGCGT GGGCGTCATT ATATCTAGAG 
GCGTGGAAGC GATATTCTGT GGAACTGGCC TTTCGTTGGG GTACCCTATC GACACCCCCG 
GAACTACTCG AACCACCTAG ACCACTCTAT AAGGGTAGTT TAGTAGAAAA TAATGTAACT 
GGACGACTTG AACCAAGGGA AGCGCCCGCG TGGAAGCGTC GTGCTTTTCG TTACCTAGTT 
AGTTTTCCAG TGATCGGAAT GTGCCTAGCC CTTGTTTTTG TGGTTATGTT CCTGATGCTA 
CGATTCCAGG ATTGGCTGGA TCATAATAAC ATACCTGATA GAGGAATTGC TCAATGCATG 
TATAATTTGC ACAAGGATTG GTGGGACTCC AAATTACCGG AAGAGAGCAT TCTCTGTTGT 
TTGAGCGTAA TACCAAAGGT TTTATTGGCT GGTGCCATAA CATTAATGGA TGAGGCATAT 
TATAAATTGG CTGTCTGGCT AAATGATCAC GAAAACTATC GCCTTCAGTC CAAATACGAA 
AATCACCTTA TTGCAAAAGT GGCACTTTTT CAATTCGTCA ACTCATTTCT GTCCTTGTTC 
TATATCGCTT TCTATCTCCG AGATGAGGAT AAATTGAAAG AGCAATTGGC CGGTTTGTTA 
ATTTCACGTC AAATAATTGG TAACCTACGT GAATCGGCAA TACCTTACTT TATTGAACAA 
TGGAAACTAG CTAAATTGAG CTTTAACATG TGGGGAGCAC TAAGCCCTAC GCAAGACATC 
AATCGAACCA TAATGGATTC CGGTGTTTCA ACGACCACAT CAACAGCATC CACTACCACG 
GAAAAAGTCA AAGTGGATAC ACCGCAACAG ACGCCGACAA AGCATGTCGC CAGTAGTCCA 
TTGGTAAGAA CCACAACTTC ATCTAATAAG CGGAATATCG GTCAAGCAGA GATCGAAAGT 
ACACTTTACA AGTACGATGG TACATTTTCC GATCATTTGG AGATGTTGGT GCAGATGGGT 
TATGTAGTAC TGTTCTCTGC TGCTTTTCCA CTGGCCGGTA TATGTGCACT CGTCAACAAC 
TTGATGGAGA TACGCTCGGA TGCCTTTAAG CTGGCTCACG TTCATCAAAG ACCTTTCGGG 
CAGCGTGTAG CTAATATTGG CACTTGGCAA AACGCGTTTA GTATTCTGTC ACTGGCTGCC 
GTCATTGTAA ACTGTGCGTT GATTGGCTTA TCAGGACAAG TTTCGCGCCT ATGGCCAGGT 
CTAACAACTG CTCAAACCAT AATTTTGATT GTCACTTTGG AGCATATTAT GTTGGGATTG 
CGTTCAGCGC TTACATGGCT GCTGCCCGAA TTGCCATCAT GGTTGGCTGC TGAAATCGCA 
CGCGCCGAAC ATTGTCGCAG AGAGATGCAA TGCAAGGGCA CTTCGCCGCG GCCCACACCA 
CCGACACCGC CATCGACTAC ATCAACGCAA CCAGACCAGG ATGACTTAAT TTTAGAAGCC 
ACCACAACGA ATGATCAATC AATGGAGAGT CAAGTTGCTG AGGATATTTT ATATCAAGAA 
CAACTGCGTC AAAGCTCTAA CTATCCGCAA ATGGATGAGA TAGTCAATTT CGGTCTACAA 
CGTAATATCG AAGCAGATGT GAATATATTC GAGAATCGTG ATTCCTCGCC AGACTCACCG 
CCATCACAAA CCAGTACGAT ACTGATAAGA CCTCTACATG AATCAACACC ACCAAATAGT 
GGTTCATCAG TGACGAGCTT AATTCAGGAT GCTGGCCATG GACACGGCAG CGCATGTAAA 
AGTTGTAAGC TGAAAATGAA AAAAGCGGAA ATACCCCCAT TTCATGGATA TTCAGTTCGT 
AATTTAAGCT CAATTCAAGC TAATCAAAGA TTGCACGCTT TTTCAGGAGC TTCTCTGAGC 
AGTGCAAGCA CCCTGGCATT AAATACTATC CGCCAGCCCA TACATATACC AGAAATACCG 
CCTTTCCGGA AAAAATCCAG TGACTCAGCT GATCATACTG GTAGCAGTGC GAGCAGTAGT 
CCGCAGCGCT CCAGACATCC GCTTATTTCA ACACCTCCAC CATCCATGTT ACAGCATCAA 
CCCACAAAAA TACCGGAGAT ACCACCTTAT AAACAACGCA AAATCTCGGC TGAAAGTGCC 
ACACATATAC ATATGAGTGA TAAGTTTATG CTCAAATCAC ACGCACCCGA GTGGATGTCC 
CGCTTGAAAG TCAGCGATAA TATAAACTTG CATAGGAGCA TAGATTGTAT TGCAAAGGAG 
TTACAAAGTA ATACAGACAC AGCGGACATC TTAAAGCCAG CACCATCCTG GAGTAATGTA 
GCTATAAAAT CGCCTACACA ACAGCAAGTG GCTGCCTCCT CATCATCCTC ATCCGCTGGT 
GGCGGCAGTT CGGGCAGTGC TGGCGGCATG GGAGTAAGTG TCGGCATTGT TGGGCGTGCT 
GTAGACAGTG GCCGACCTAG TGTGGGTGGT ATTTCTACTA CGTCGACCTC ATCGATGCAA 
CTGAAAAAAC TAACTGACAA TGTCAAGGAT ACAACATCAG CAGAGATAGT CCCAGTAAAA 
GGGAGTTCTG GTGGTGCAGG CGCAGGAAGT ACAACACAAA GTGGCAGCAC AGACGATGAA 
GCTAAAGCAG CGGAGATTGC AGCGAAAAAA TCGCGATTAA AGCAAAAGCT TGTGAAAAGC 
GCGCGATCAG TTGGCATATT CTCGCTCAAA CTGAAAGAGC GACGACAGCG CGAGGCGGAG 
AAGGCGGCAA CTATTGCCAT TGAACATGCC AAGGCTTTGG CTAAATTACC ACCCCTGCCG 
CAACCAGGCG GTGAGCTGTC TCTCATACCT ATTGAACAGC TAATACAAAT TGAAGATATA 
ATACCGACAC CGAAACCACA ACCCACCACA TCGTCGGCAA CAACGATGTC AGCGGCGGCA 
AGTTTGTCCA CTGCTGTGAC GTCACCACAA GCAATGACAC CCTCATCGAG CGCACTGGCG 
GCCGCAATGC AGCAATTTCA AAGTTCGGGA AATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217978 ( XM_011193276.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191578.2 Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa257546
Clone ID Related Accession (Same CDS sequence)	XM_011193276.2
Accession Version	XM_011193276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105217978 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011193276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGTGGGACT CCAAATTACC GGAAGAGAGC 
ATTCTCTGTT GTTTGAGCGT AATACCAAAG GTTTTATTGG CTGGTGCCAT AACATTAATG 
GATGAGGCAT ATTATAAATT GGCTGTCTGG CTAAATGATC ACGAAAACTA TCGCCTTCAG 
TCCAAATACG AAAATCACCT TATTGCAAAA GTGGCACTTT TTCAATTCGT CAACTCATTT 
CTGTCCTTGT TCTATATCGC TTTCTATCTC CGAGATGAGG ATAAATTGAA AGAGCAATTG 
GCCGGTTTGT TAATTTCACG TCAAATAATT GGTAACCTAC GTGAATCGGC AATACCTTAC 
TTTATTGAAC AATGGAAACT AGCTAAATTG AGCTTTAACA TGTGGGGAGC ACTAAGCCCT 
ACGCAAGACA TCAATCGAAC CATAATGGAT TCCGGTGTTT CAACGACCAC ATCAACAGCA 
TCCACTACCA CGGAAAAAGT CAAAGTGGAT ACACCGCAAC AGACGCCGAC AAAGCATGTC 
GCCAGTAGTC CATTGGTAAG AACCACAACT TCATCTAATA AGCGGAATAT CGGTCAAGCA 
GAGATCGAAA GTACACTTTA CAAGTACGAT GGTACATTTT CCGATCATTT GGAGATGTTG 
GTGCAGATGG GTTATGTAGT ACTGTTCTCT GCTGCTTTTC CACTGGCCGG TATATGTGCA 
CTCGTCAACA ACTTGATGGA GATACGCTCG GATGCCTTTA AGCTGGCTCA CGTTCATCAA 
AGACCTTTCG GGCAGCGTGT AGCTAATATT GGCACTTGGC AAAACGCGTT TAGTATTCTG 
TCACTGGCTG CCGTCATTGT AAACTGTGCG TTGATTGGCT TATCAGGACA AGTTTCGCGC 
CTATGGCCAG GTCTAACAAC TGCTCAAACC ATAATTTTGA TTGTCACTTT GGAGCATATT 
ATGTTGGGAT TGCGTTCAGC GCTTACATGG CTGCTGCCCG AATTGCCATC ATGGTTGGCT 
GCTGAAATCG CACGCGCCGA ACATTGTCGC AGAGAGATGC AATGCAAGGG CACTTCGCCG 
CGGCCCACAC CACCGACACC GCCATCGACT ACATCAACGC AACCAGACCA GGATGACTTA 
ATTTTAGAAG CCACCACAAC GAATGATCAA TCAATGGAGA GTCAAGTTGC TGAGGATATT 
TTATATCAAG AACAACTGCG TCAAAGCTCT AACTATCCGC AAATGGATGA GATAGTCAAT 
TTCGGTCTAC AACGTAATAT CGAAGCAGAT GTGAATATAT TCGAGAATCG TGATTCCTCG 
CCAGACTCAC CGCCATCACA AACCAGTACG ATACTGATAA GACCTCTACA TGAATCAACA 
CCACCAAATA GTGGTTCATC AGTGACGAGC TTAATTCAGG ATGCTGGCCA TGGACACGGC 
AGCGCATGTA AAAGTTGTAA GCTGAAAATG AAAAAAGCGG AAATACCCCC ATTTCATGGA 
TATTCAGTTC GTAATTTAAG CTCAATTCAA GCTAATCAAA GATTGCACGC TTTTTCAGGA 
GCTTCTCTGA GCAGTGCAAG CACCCTGGCA TTAAATACTA TCCGCCAGCC CATACATATA 
CCAGAAATAC CGCCTTTCCG GAAAAAATCC AGTGACTCAG CTGATCATAC TGGTAGCAGT 
GCGAGCAGTA GTCCGCAGCG CTCCAGACAT CCGCTTATTT CAACACCTCC ACCATCCATG 
TTACAGCATC AACCCACAAA AATACCGGAG ATACCACCTT ATAAACAACG CAAAATCTCG 
GCTGAAAGTG CCACACATAT ACATATGAGT GAGTTACAAA GTAATACAGA CACAGCGGAC 
ATCTTAAAGC CAGCACCATC CTGGAGTAAT GTAGCTATAA AATCGCCTAC ACAACAGCAA 
GTGGCTGCCT CCTCATCATC CTCATCCGCT GGTGGCGGCA GTTCGGGCAG TGCTGGCGGC 
ATGGGAGTAA GTGTCGGCAT TGTTGGGCGT GCTGTAGACA GTGGCCGACC TAGTGTGGGT 
GGTATTTCTA CTACGTCGAC CTCATCGATG CAACTGAAAA AACTAACTGA CAATGTCAAG 
GATACAACAT CAGCAGAGAT AGTCCCAGTA AAAGGGAGTT CTGGTGGTGC AGGCGCAGGA 
AGTACAACAC AAAGTGGCAG CACAGACGAT GAAGCTAAAG CAGCGGAGAT TGCAGCGAAA 
AAATCGCGAT TAAAGCAAAA GCTTGTGAAA AGCGCGCGAT CAGTTGGCAT ATTCTCGCTC 
AAACTGAAAG AGCGACGACA GCGCGAGGCG GAGAAGGCGG CAACTATTGC CATTGAACAT 
GCCAAGGCTT TGGCTAAATT ACCACCCCTG CCGCAACCAG GCGGTGAGCT GTCTCTCATA 
CCTATTGAAC AGCTAATACA AATTGAAGAT ATAATACCGA CACCGAAACC ACAACCCACC 
ACATCGTCGG CAACAACGAT GTCAGCGGCG GCAAGTTTGT CCACTGCTGT GACGTCACCA 
CAAGCAATGA CACCCTCATC GAGCGCACTG GCGGCCGCAA TGCAGCAATT TCAAAGTTCG 
GGAAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191578.2
CDS	448..4299
Translation	MDKSFENLLQSGGGGGDMSSERQSSEDVHESTAEDAGLDAGDDFTDDTNFESNLRRRKGKIIACAKETIENASRQLRRKVPYAGHLMTPRRLWLNKIPTQCDVVIEFPEDAPDEALRWLLSRLRSQPPSGLGLVVQVKAHESTRRNAFYISAPTNVLFKAAEEARLPKRLRPDLGGALREFTTRESHCFQQLKTEDGSSALFTSQERQWLVLQVLQGLRASQADIEALQGRATVAEGQSIVAAWQETGLIVQVFPLHETRSLTLLQSSWVKQIFAPQPLDDIAEYFGVKVALYFAWLGHYTCALGVPAVFGTILYAALWGKGQTAQDMGHVLFSLFNVAWASLYLEAWKRYSVELAFRWGTLSTPPELLEPPRPLYKGSLVENNVTGRLEPREAPAWKRRAFRYLVSFPVIGMCLALVFVVMFLMLRFQDWWDSKLPEESILCCLSVIPKVLLAGAITLMDEAYYKLAVWLNDHENYRLQSKYENHLIAKVALFQFVNSFLSLFYIAFYLRDEDKLKEQLAGLLISRQIIGNLRESAIPYFIEQWKLAKLSFNMWGALSPTQDINRTIMDSGVSTTTSTASTTTEKVKVDTPQQTPTKHVASSPLVRTTTSSNKRNIGQAEIESTLYKYDGTFSDHLEMLVQMGYVVLFSAAFPLAGICALVNNLMEIRSDAFKLAHVHQRPFGQRVANIGTWQNAFSILSLAAVIVNCALIGLSGQVSRLWPGLTTAQTIILIVTLEHIMLGLRSALTWLLPELPSWLAAEIARAEHCRREMQCKGTSPRPTPPTPPSTTSTQPDQDDLILEATTTNDQSMESQVAEDILYQEQLRQSSNYPQMDEIVNFGLQRNIEADVNIFENRDSSPDSPPSQTSTILIRPLHESTPPNSGSSVTSLIQDAGHGHGSACKSCKLKMKKAEIPPFHGYSVRNLSSIQANQRLHAFSGASLSSASTLALNTIRQPIHIPEIPPFRKKSSDSADHTGSSASSSPQRSRHPLISTPPPSMLQHQPTKIPEIPPYKQRKISAESATHIHMSELQSNTDTADILKPAPSWSNVAIKSPTQQQVAASSSSSSAGGGSSGSAGGMGVSVGIVGRAVDSGRPSVGGISTTSTSSMQLKKLTDNVKDTTSAEIVPVKGSSGGAGAGSTTQSGSTDDEAKAAEIAAKKSRLKQKLVKSARSVGIFSLKLKERRQREAEKAATIAIEHAKALAKLPPLPQPGGELSLIPIEQLIQIEDIIPTPKPQPTTSSATTMSAAASLSTAVTSPQAMTPSSSALAAAMQQFQSSGNH

Target ORF information:

RefSeq Version	XM_011193276.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105217978 (LOC105217978), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193276.2

ORF Insert Sequence:

ATGGACAAAA GTTTTGAAAA TCTATTACAA AGCGGTGGCG GCGGTGGTGA CATGTCCTCC 
GAACGACAAT CGTCCGAAGA TGTACACGAA TCGACAGCGG AGGATGCCGG CTTGGATGCC 
GGAGATGACT TTACCGATGA CACTAACTTC GAATCAAATC TGCGTAGACG CAAGGGTAAA 
ATCATTGCGT GTGCCAAGGA AACGATTGAA AATGCGTCCC GTCAATTACG TCGAAAAGTA 
CCATATGCTG GACATTTAAT GACGCCGCGT CGTCTTTGGT TGAATAAAAT ACCGACACAA 
TGTGATGTTG TCATCGAGTT TCCAGAAGAT GCGCCTGACG AGGCGCTACG TTGGCTTCTC 
TCCAGATTGC GTTCTCAGCC ACCATCGGGG CTTGGATTGG TCGTACAAGT GAAGGCTCAT 
GAGAGTACAC GGCGGAATGC TTTCTATATT AGTGCACCAA CGAATGTTTT ATTTAAAGCG 
GCCGAAGAAG CACGATTACC GAAACGCTTA CGTCCTGACC TAGGTGGTGC CTTACGTGAA 
TTCACAACAC GCGAAAGTCA TTGTTTTCAG CAACTGAAAA CTGAAGATGG GAGCAGTGCA 
CTTTTCACTT CACAAGAGCG TCAATGGCTG GTGCTGCAGG TATTGCAAGG CCTCCGTGCC 
AGTCAAGCGG ATATTGAGGC CCTACAGGGG CGTGCAACTG TAGCCGAAGG GCAAAGCATA 
GTTGCAGCCT GGCAAGAGAC TGGACTGATT GTGCAGGTTT TTCCGCTGCA TGAAACCCGC 
TCGCTGACGC TGCTACAAAG CAGTTGGGTG AAACAGATAT TTGCGCCGCA ACCGCTTGAT 
GATATAGCTG AATATTTTGG TGTGAAGGTG GCGCTTTACT TTGCTTGGCT GGGCCATTAC 
ACCTGCGCCT TAGGCGTGCC AGCTGTCTTT GGCACAATAT TGTATGCAGC ACTCTGGGGA 
AAAGGACAAA CAGCACAAGA TATGGGACAC GTTTTATTCT CACTCTTTAA TGTAGCGTGG 
GCGTCATTAT ATCTAGAGGC GTGGAAGCGA TATTCTGTGG AACTGGCCTT TCGTTGGGGT 
ACCCTATCGA CACCCCCGGA ACTACTCGAA CCACCTAGAC CACTCTATAA GGGTAGTTTA 
GTAGAAAATA ATGTAACTGG ACGACTTGAA CCAAGGGAAG CGCCCGCGTG GAAGCGTCGT 
GCTTTTCGTT ACCTAGTTAG TTTTCCAGTG ATCGGAATGT GCCTAGCCCT TGTTTTTGTG 
GTTATGTTCC TGATGCTACG ATTCCAGGAT TGGTGGGACT CCAAATTACC GGAAGAGAGC 
ATTCTCTGTT GTTTGAGCGT AATACCAAAG GTTTTATTGG CTGGTGCCAT AACATTAATG 
GATGAGGCAT ATTATAAATT GGCTGTCTGG CTAAATGATC ACGAAAACTA TCGCCTTCAG 
TCCAAATACG AAAATCACCT TATTGCAAAA GTGGCACTTT TTCAATTCGT CAACTCATTT 
CTGTCCTTGT TCTATATCGC TTTCTATCTC CGAGATGAGG ATAAATTGAA AGAGCAATTG 
GCCGGTTTGT TAATTTCACG TCAAATAATT GGTAACCTAC GTGAATCGGC AATACCTTAC 
TTTATTGAAC AATGGAAACT AGCTAAATTG AGCTTTAACA TGTGGGGAGC ACTAAGCCCT 
ACGCAAGACA TCAATCGAAC CATAATGGAT TCCGGTGTTT CAACGACCAC ATCAACAGCA 
TCCACTACCA CGGAAAAAGT CAAAGTGGAT ACACCGCAAC AGACGCCGAC AAAGCATGTC 
GCCAGTAGTC CATTGGTAAG AACCACAACT TCATCTAATA AGCGGAATAT CGGTCAAGCA 
GAGATCGAAA GTACACTTTA CAAGTACGAT GGTACATTTT CCGATCATTT GGAGATGTTG 
GTGCAGATGG GTTATGTAGT ACTGTTCTCT GCTGCTTTTC CACTGGCCGG TATATGTGCA 
CTCGTCAACA ACTTGATGGA GATACGCTCG GATGCCTTTA AGCTGGCTCA CGTTCATCAA 
AGACCTTTCG GGCAGCGTGT AGCTAATATT GGCACTTGGC AAAACGCGTT TAGTATTCTG 
TCACTGGCTG CCGTCATTGT AAACTGTGCG TTGATTGGCT TATCAGGACA AGTTTCGCGC 
CTATGGCCAG GTCTAACAAC TGCTCAAACC ATAATTTTGA TTGTCACTTT GGAGCATATT 
ATGTTGGGAT TGCGTTCAGC GCTTACATGG CTGCTGCCCG AATTGCCATC ATGGTTGGCT 
GCTGAAATCG CACGCGCCGA ACATTGTCGC AGAGAGATGC AATGCAAGGG CACTTCGCCG 
CGGCCCACAC CACCGACACC GCCATCGACT ACATCAACGC AACCAGACCA GGATGACTTA 
ATTTTAGAAG CCACCACAAC GAATGATCAA TCAATGGAGA GTCAAGTTGC TGAGGATATT 
TTATATCAAG AACAACTGCG TCAAAGCTCT AACTATCCGC AAATGGATGA GATAGTCAAT 
TTCGGTCTAC AACGTAATAT CGAAGCAGAT GTGAATATAT TCGAGAATCG TGATTCCTCG 
CCAGACTCAC CGCCATCACA AACCAGTACG ATACTGATAA GACCTCTACA TGAATCAACA 
CCACCAAATA GTGGTTCATC AGTGACGAGC TTAATTCAGG ATGCTGGCCA TGGACACGGC 
AGCGCATGTA AAAGTTGTAA GCTGAAAATG AAAAAAGCGG AAATACCCCC ATTTCATGGA 
TATTCAGTTC GTAATTTAAG CTCAATTCAA GCTAATCAAA GATTGCACGC TTTTTCAGGA 
GCTTCTCTGA GCAGTGCAAG CACCCTGGCA TTAAATACTA TCCGCCAGCC CATACATATA 
CCAGAAATAC CGCCTTTCCG GAAAAAATCC AGTGACTCAG CTGATCATAC TGGTAGCAGT 
GCGAGCAGTA GTCCGCAGCG CTCCAGACAT CCGCTTATTT CAACACCTCC ACCATCCATG 
TTACAGCATC AACCCACAAA AATACCGGAG ATACCACCTT ATAAACAACG CAAAATCTCG 
GCTGAAAGTG CCACACATAT ACATATGAGT GAGTTACAAA GTAATACAGA CACAGCGGAC 
ATCTTAAAGC CAGCACCATC CTGGAGTAAT GTAGCTATAA AATCGCCTAC ACAACAGCAA 
GTGGCTGCCT CCTCATCATC CTCATCCGCT GGTGGCGGCA GTTCGGGCAG TGCTGGCGGC 
ATGGGAGTAA GTGTCGGCAT TGTTGGGCGT GCTGTAGACA GTGGCCGACC TAGTGTGGGT 
GGTATTTCTA CTACGTCGAC CTCATCGATG CAACTGAAAA AACTAACTGA CAATGTCAAG 
GATACAACAT CAGCAGAGAT AGTCCCAGTA AAAGGGAGTT CTGGTGGTGC AGGCGCAGGA 
AGTACAACAC AAAGTGGCAG CACAGACGAT GAAGCTAAAG CAGCGGAGAT TGCAGCGAAA 
AAATCGCGAT TAAAGCAAAA GCTTGTGAAA AGCGCGCGAT CAGTTGGCAT ATTCTCGCTC 
AAACTGAAAG AGCGACGACA GCGCGAGGCG GAGAAGGCGG CAACTATTGC CATTGAACAT 
GCCAAGGCTT TGGCTAAATT ACCACCCCTG CCGCAACCAG GCGGTGAGCT GTCTCTCATA 
CCTATTGAAC AGCTAATACA AATTGAAGAT ATAATACCGA CACCGAAACC ACAACCCACC 
ACATCGTCGG CAACAACGAT GTCAGCGGCG GCAAGTTTGT CCACTGCTGT GACGTCACCA 
CAAGCAATGA CACCCTCATC GAGCGCACTG GCGGCCGCAA TGCAGCAATT TCAAAGTTCG 
GGAAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC105217978 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

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