LOC105213002 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105213002
Entrez Gene ID	105213002
Full Name	ubiquitin protein ligase E3B
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011185475.1	XP_011183777.1	ubiquitin-protein ligase E3B
XM_011185475.2	XP_011183777.1	ubiquitin-protein ligase E3B

Pathways from BioSystems
Gene Ontology

The following LOC105213002 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105213002 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa199388	XM_011185475.2 Latest version!	Zeugodacus cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBa199388	XM_011185475.1	Bactrocera cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105213002 ( XM_011185475.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183777.1 Bactrocera cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.

Price & Availability↑

CloneID	OBa199388
Clone ID Related Accession (Same CDS sequence)	XM_011185475.1 , XM_011185475.2
Accession Version	XM_011185475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	ubiquitin-protein ligase E3B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863711.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTA CGTCTGATTC GGAAAAAAAG TGTTTTTTGG AGCATACCAA AGCTGCTAGA 
GAGGAGCGTG CATACGAAAA ACGTCGGGAA GAAGCAGCTG TGAAAATACA AGCACACTGG 
AAAGGATATA CAACTCGTAA ACAGTATTAC AATAAGATAA TCTCCGACTT TGATGTTATA 
TTCATAAAGG AAAATAATGA TTTAGACGTG GATCTTTTAC CATCAAATAC CGTGTATCCG 
GTTATTCGTC GCTTTCTCAC ATATTTTGAA TATAAAAATG GATCGCCAAC CGTAGTGAAA 
CCGTCACGTG AAAAGTTTGA ATGTTTATGT CGTTATTTGA ACAAATCTAT GGAATCGGAA 
TCACCAAAAT TGTCATACGC AGCTCTTTGT CTGCACAAAG ATAAGAGCTT GCCTTGGTTG 
GCACATATCA AATCATTATT GAGTTTATGC TGTTTAATGC TGGACGATAT TAAGCCAGAA 
AATCATACGG ACAGCATTTC TCTGGCTCTG TATCTACATA CTTTGATTGT TTTCACTTCA 
CCGAAGTCAT GGGGTATTCT ACGAAATAAA CAATTCGAAA AAATGCAACC AGTATTTCAG 
AAAATATGCT GCAATATTCA AGGGAAATTA GTACAAAATA ATTTTTTCAA AACTATGCGC 
TCAATCCTAT TGAAGGGTAC AGCACGGGAG GATCTTTCCA TAAAACCAGT TTCCCTTAAA 
GCTATAATGA CGCTTTCTAC CCGACCGCTT ATCGATGGGG GCTATAGTCG TAATTTACTC 
ACATATTTTT TCGCCGAAAT TTTATCCGTT CCAGCATTGG TATTCCATTT GAATATTGTT 
ACCTCACAGT GCTTGGAAAA TCTCATTACC ATGCATATCC TCCAAAGATT TGTTCAATCA 
GCAGATGATT CTAATTGGTT TTCAGAATTT ACAAACAGTA TGCCCGGGAC GAAATTACTT 
GCATTTTTGG GGAATATTGT GAATCTTTTT ACACTCGAAA GCTCCGTGGA AGCAAAAGAG 
CTTGCATATC CTCTTTTCAC GAGTGCAACC ACTATACTTC TCGAAAATAT TCCTAACACG 
GTATCAAGTC GAGGAGTATT CACGCAATGG CATGAACTTC TTGGATGGCA TACTCCTTCG 
TTGGATTCGG CACAAAATCA AAATGTAGCT CTAATTAAAA AGCAATTTCA TTTGTTATGG 
GGTCATCGGT GTATCAAAGT TTTATTGGCT GATTTTCTGA GGGATATTAA TGCTACTTAT 
GAGCGAATAG AATTCCAGCC CCCAGTACAA GCATCCACAA GCAACATACT TCGTCGCGCG 
TTGGACCGCG GTTCAGCTCG CGGTACTTTT ATCAGAGGCA ATAGAACATG GCGTCGATTA 
GATTGTATCG AGGTAGAGCA GGTGTCACGG GTATGCGCAA TGTTTTATGC GGCACTAAAT 
ACGCTTTCAC AACTTCGACT TGATATTTTA ACAGGTATTT GTTACAATGA TAATGTTTTG 
TACGATCTTT GGCTACTTCT CACTTCGCTG GGGCCGAATT GTGGCATGAA GGAGTTTATG 
GAGTTATTAA AGCAAGACAA CGTTTTACAA AAATCCCAGG TCACTATGCT CATGCTCTTT 
TGTGACAGTA TGACCCATTA TGTAACTATT TTGGATGAGT ATGAAATGTA CACAGAACAA 
AAACCATTTC TCCTCAATGA TTTTATAATG CTTACTTATT TTCTCAATAA TATACTGTAC 
AAACTAATTA ACGAGAATTT ACTCGGTACG AAAAATGTCA CCCAATGTCC GGTATTTGTA 
TCACTGCATA CGTTACTGTT ATGTCTATAC CGTCGTGATT GTCGTCGATC ATTTGCGCCA 
ACTAACCACT GGCTGATTCC CGAAGTAAAG CCCTCTGCAT TTATCAGTGA TTTGGAGAAA 
AACAAGAAAC ACGCAGTGAT TTTATTGCAG AAAATGCCAC ATATAATACC ACATGAAGAT 
CGCGTCAAAT TGTTTCGTAA ATTTGTACAA AATGAAAAGG CAGTGATGGG TTTGACGGAG 
AGTGCGTGTG CATCACCACG ATCTGCTTTA ATCGTCGTAC ATCGTGATCG CATTGTGGAG 
GATGGCTATA GACAGTTGGC AGCACTTAGG CCACATGCTC TGAAAGGCGT GATTCGTGTA 
CGTTTTATTA ATCAACAAGG TTTGCACGAG GCCGGTATTG ATCAAGATGG CGTTTTCAAG 
GAATTCTTAG AAGAAACAAT TAAGAGAGTA TTTGATCCAT CACTGAATTT ATTTAAAACC 
ACAACGGATC AGCGTTTATA TCCTTCGCCT ATATCATATG TACAGGACAA TCATTTGCAA 
TTGTTCGAGT TTGTAGGACG AATGTTGGGA AAAGCAGTAT ATGAAGGTAT AGTTGTGGAT 
GTTCCATTCG CTTCCTTCTT TCTCTCCCAG CTCTTAGGCC AAACACATCA GGCACTGTAC 
TCTTGTATGG ATGAGCTTCC CTCATTGGAC AACGAGCTCT ATCGCAGCCT TACTTTCATT 
AAGCACTATA AACAAGACGT TGCTGACTTG AATTTAACAT TTTCGGTAGA CCAAGATGTA 
ATGGGAAAGA TTGTTACACA TGAATTGCAT CCAGGTGGAA AAGCTCGTGC TGTTACTGAC 
AGCAACAAAC TAGTATACAT ACATTATATG GCCTATTTTC ATATGAACAC GCAAATACGA 
GAACAAACAC AGGCTTTCAA TCGTGGATTT CGTAGTATCG TCAACCCTGA ATGGTTATCC 
TTATTTTCAC CACCGGAATT ACAGCGCTTA ATATCTGGTG ATACTGTACC GCTGGATTTG 
CGAGATCTAC GAAAACATAC GCAGTACTAT GGAGGATTTC ATGATTCCCA CCGTGTCGTT 
GGTTGGTTAT GGGATATTTT GGCCAAAGAT TTTACGGAAG AAGAGCGGAA ATTATTTCTT 
AAGTTTGTTA CTAGTTGTTC CAAGCCACCT TTACTTGGAT TTGCCAATCT CGAGCCACCG 
TTTTCCATTC GTTGTGTTGA AGTTAGTGAT GACGAAGATA CTGGTGACAC TATTGGTAGT 
GTCATACGTG GTTTTTTTAC AATACGCAAG AAAGACCCCC TCAATCGGTT GCCGACATCA 
TCAACTTGCT TTAATTTATT GAAGTTGCCA AACTACCAAA AGAAGTCCAC GTTGCGCGAC 
AAATTACGTT ACGCTGTGAG CAGCAATACC GGATTTGAAT TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183777.1
CDS	155..3382
Translation	MFVTSDSEKKCFLEHTKAAREERAYEKRREEAAVKIQAHWKGYTTRKQYYNKIISDFDVIFIKENNDLDVDLLPSNTVYPVIRRFLTYFEYKNGSPTVVKPSREKFECLCRYLNKSMESESPKLSYAALCLHKDKSLPWLAHIKSLLSLCCLMLDDIKPENHTDSISLALYLHTLIVFTSPKSWGILRNKQFEKMQPVFQKICCNIQGKLVQNNFFKTMRSILLKGTAREDLSIKPVSLKAIMTLSTRPLIDGGYSRNLLTYFFAEILSVPALVFHLNIVTSQCLENLITMHILQRFVQSADDSNWFSEFTNSMPGTKLLAFLGNIVNLFTLESSVEAKELAYPLFTSATTILLENIPNTVSSRGVFTQWHELLGWHTPSLDSAQNQNVALIKKQFHLLWGHRCIKVLLADFLRDINATYERIEFQPPVQASTSNILRRALDRGSARGTFIRGNRTWRRLDCIEVEQVSRVCAMFYAALNTLSQLRLDILTGICYNDNVLYDLWLLLTSLGPNCGMKEFMELLKQDNVLQKSQVTMLMLFCDSMTHYVTILDEYEMYTEQKPFLLNDFIMLTYFLNNILYKLINENLLGTKNVTQCPVFVSLHTLLLCLYRRDCRRSFAPTNHWLIPEVKPSAFISDLEKNKKHAVILLQKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVVHRDRIVEDGYRQLAALRPHALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKRVFDPSLNLFKTTTDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTHQALYSCMDELPSLDNELYRSLTFIKHYKQDVADLNLTFSVDQDVMGKIVTHELHPGGKARAVTDSNKLVYIHYMAYFHMNTQIREQTQAFNRGFRSIVNPEWLSLFSPPELQRLISGDTVPLDLRDLRKHTQYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFANLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_011185475.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185475.1

ORF Insert Sequence:

ATGTTTGTTA CGTCTGATTC GGAAAAAAAG TGTTTTTTGG AGCATACCAA AGCTGCTAGA 
GAGGAGCGTG CATACGAAAA ACGTCGGGAA GAAGCAGCTG TGAAAATACA AGCACACTGG 
AAAGGATATA CAACTCGTAA ACAGTATTAC AATAAGATAA TCTCCGACTT TGATGTTATA 
TTCATAAAGG AAAATAATGA TTTAGACGTG GATCTTTTAC CATCAAATAC CGTGTATCCG 
GTTATTCGTC GCTTTCTCAC ATATTTTGAA TATAAAAATG GATCGCCAAC CGTAGTGAAA 
CCGTCACGTG AAAAGTTTGA ATGTTTATGT CGTTATTTGA ACAAATCTAT GGAATCGGAA 
TCACCAAAAT TGTCATACGC AGCTCTTTGT CTGCACAAAG ATAAGAGCTT GCCTTGGTTG 
GCACATATCA AATCATTATT GAGTTTATGC TGTTTAATGC TGGACGATAT TAAGCCAGAA 
AATCATACGG ACAGCATTTC TCTGGCTCTG TATCTACATA CTTTGATTGT TTTCACTTCA 
CCGAAGTCAT GGGGTATTCT ACGAAATAAA CAATTCGAAA AAATGCAACC AGTATTTCAG 
AAAATATGCT GCAATATTCA AGGGAAATTA GTACAAAATA ATTTTTTCAA AACTATGCGC 
TCAATCCTAT TGAAGGGTAC AGCACGGGAG GATCTTTCCA TAAAACCAGT TTCCCTTAAA 
GCTATAATGA CGCTTTCTAC CCGACCGCTT ATCGATGGGG GCTATAGTCG TAATTTACTC 
ACATATTTTT TCGCCGAAAT TTTATCCGTT CCAGCATTGG TATTCCATTT GAATATTGTT 
ACCTCACAGT GCTTGGAAAA TCTCATTACC ATGCATATCC TCCAAAGATT TGTTCAATCA 
GCAGATGATT CTAATTGGTT TTCAGAATTT ACAAACAGTA TGCCCGGGAC GAAATTACTT 
GCATTTTTGG GGAATATTGT GAATCTTTTT ACACTCGAAA GCTCCGTGGA AGCAAAAGAG 
CTTGCATATC CTCTTTTCAC GAGTGCAACC ACTATACTTC TCGAAAATAT TCCTAACACG 
GTATCAAGTC GAGGAGTATT CACGCAATGG CATGAACTTC TTGGATGGCA TACTCCTTCG 
TTGGATTCGG CACAAAATCA AAATGTAGCT CTAATTAAAA AGCAATTTCA TTTGTTATGG 
GGTCATCGGT GTATCAAAGT TTTATTGGCT GATTTTCTGA GGGATATTAA TGCTACTTAT 
GAGCGAATAG AATTCCAGCC CCCAGTACAA GCATCCACAA GCAACATACT TCGTCGCGCG 
TTGGACCGCG GTTCAGCTCG CGGTACTTTT ATCAGAGGCA ATAGAACATG GCGTCGATTA 
GATTGTATCG AGGTAGAGCA GGTGTCACGG GTATGCGCAA TGTTTTATGC GGCACTAAAT 
ACGCTTTCAC AACTTCGACT TGATATTTTA ACAGGTATTT GTTACAATGA TAATGTTTTG 
TACGATCTTT GGCTACTTCT CACTTCGCTG GGGCCGAATT GTGGCATGAA GGAGTTTATG 
GAGTTATTAA AGCAAGACAA CGTTTTACAA AAATCCCAGG TCACTATGCT CATGCTCTTT 
TGTGACAGTA TGACCCATTA TGTAACTATT TTGGATGAGT ATGAAATGTA CACAGAACAA 
AAACCATTTC TCCTCAATGA TTTTATAATG CTTACTTATT TTCTCAATAA TATACTGTAC 
AAACTAATTA ACGAGAATTT ACTCGGTACG AAAAATGTCA CCCAATGTCC GGTATTTGTA 
TCACTGCATA CGTTACTGTT ATGTCTATAC CGTCGTGATT GTCGTCGATC ATTTGCGCCA 
ACTAACCACT GGCTGATTCC CGAAGTAAAG CCCTCTGCAT TTATCAGTGA TTTGGAGAAA 
AACAAGAAAC ACGCAGTGAT TTTATTGCAG AAAATGCCAC ATATAATACC ACATGAAGAT 
CGCGTCAAAT TGTTTCGTAA ATTTGTACAA AATGAAAAGG CAGTGATGGG TTTGACGGAG 
AGTGCGTGTG CATCACCACG ATCTGCTTTA ATCGTCGTAC ATCGTGATCG CATTGTGGAG 
GATGGCTATA GACAGTTGGC AGCACTTAGG CCACATGCTC TGAAAGGCGT GATTCGTGTA 
CGTTTTATTA ATCAACAAGG TTTGCACGAG GCCGGTATTG ATCAAGATGG CGTTTTCAAG 
GAATTCTTAG AAGAAACAAT TAAGAGAGTA TTTGATCCAT CACTGAATTT ATTTAAAACC 
ACAACGGATC AGCGTTTATA TCCTTCGCCT ATATCATATG TACAGGACAA TCATTTGCAA 
TTGTTCGAGT TTGTAGGACG AATGTTGGGA AAAGCAGTAT ATGAAGGTAT AGTTGTGGAT 
GTTCCATTCG CTTCCTTCTT TCTCTCCCAG CTCTTAGGCC AAACACATCA GGCACTGTAC 
TCTTGTATGG ATGAGCTTCC CTCATTGGAC AACGAGCTCT ATCGCAGCCT TACTTTCATT 
AAGCACTATA AACAAGACGT TGCTGACTTG AATTTAACAT TTTCGGTAGA CCAAGATGTA 
ATGGGAAAGA TTGTTACACA TGAATTGCAT CCAGGTGGAA AAGCTCGTGC TGTTACTGAC 
AGCAACAAAC TAGTATACAT ACATTATATG GCCTATTTTC ATATGAACAC GCAAATACGA 
GAACAAACAC AGGCTTTCAA TCGTGGATTT CGTAGTATCG TCAACCCTGA ATGGTTATCC 
TTATTTTCAC CACCGGAATT ACAGCGCTTA ATATCTGGTG ATACTGTACC GCTGGATTTG 
CGAGATCTAC GAAAACATAC GCAGTACTAT GGAGGATTTC ATGATTCCCA CCGTGTCGTT 
GGTTGGTTAT GGGATATTTT GGCCAAAGAT TTTACGGAAG AAGAGCGGAA ATTATTTCTT 
AAGTTTGTTA CTAGTTGTTC CAAGCCACCT TTACTTGGAT TTGCCAATCT CGAGCCACCG 
TTTTCCATTC GTTGTGTTGA AGTTAGTGAT GACGAAGATA CTGGTGACAC TATTGGTAGT 
GTCATACGTG GTTTTTTTAC AATACGCAAG AAAGACCCCC TCAATCGGTT GCCGACATCA 
TCAACTTGCT TTAATTTATT GAAGTTGCCA AACTACCAAA AGAAGTCCAC GTTGCGCGAC 
AAATTACGTT ACGCTGTGAG CAGCAATACC GGATTTGAAT TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105213002 ( XM_011185475.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183777.1 Zeugodacus cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.

Price & Availability↑

CloneID	OBa199388
Clone ID Related Accession (Same CDS sequence)	XM_011185475.1 , XM_011185475.2
Accession Version	XM_011185475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ubiquitin-protein ligase E3B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863711.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTA CGTCTGATTC GGAAAAAAAG TGTTTTTTGG AGCATACCAA AGCTGCTAGA 
GAGGAGCGTG CATACGAAAA ACGTCGGGAA GAAGCAGCTG TGAAAATACA AGCACACTGG 
AAAGGATATA CAACTCGTAA ACAGTATTAC AATAAGATAA TCTCCGACTT TGATGTTATA 
TTCATAAAGG AAAATAATGA TTTAGACGTG GATCTTTTAC CATCAAATAC CGTGTATCCG 
GTTATTCGTC GCTTTCTCAC ATATTTTGAA TATAAAAATG GATCGCCAAC CGTAGTGAAA 
CCGTCACGTG AAAAGTTTGA ATGTTTATGT CGTTATTTGA ACAAATCTAT GGAATCGGAA 
TCACCAAAAT TGTCATACGC AGCTCTTTGT CTGCACAAAG ATAAGAGCTT GCCTTGGTTG 
GCACATATCA AATCATTATT GAGTTTATGC TGTTTAATGC TGGACGATAT TAAGCCAGAA 
AATCATACGG ACAGCATTTC TCTGGCTCTG TATCTACATA CTTTGATTGT TTTCACTTCA 
CCGAAGTCAT GGGGTATTCT ACGAAATAAA CAATTCGAAA AAATGCAACC AGTATTTCAG 
AAAATATGCT GCAATATTCA AGGGAAATTA GTACAAAATA ATTTTTTCAA AACTATGCGC 
TCAATCCTAT TGAAGGGTAC AGCACGGGAG GATCTTTCCA TAAAACCAGT TTCCCTTAAA 
GCTATAATGA CGCTTTCTAC CCGACCGCTT ATCGATGGGG GCTATAGTCG TAATTTACTC 
ACATATTTTT TCGCCGAAAT TTTATCCGTT CCAGCATTGG TATTCCATTT GAATATTGTT 
ACCTCACAGT GCTTGGAAAA TCTCATTACC ATGCATATCC TCCAAAGATT TGTTCAATCA 
GCAGATGATT CTAATTGGTT TTCAGAATTT ACAAACAGTA TGCCCGGGAC GAAATTACTT 
GCATTTTTGG GGAATATTGT GAATCTTTTT ACACTCGAAA GCTCCGTGGA AGCAAAAGAG 
CTTGCATATC CTCTTTTCAC GAGTGCAACC ACTATACTTC TCGAAAATAT TCCTAACACG 
GTATCAAGTC GAGGAGTATT CACGCAATGG CATGAACTTC TTGGATGGCA TACTCCTTCG 
TTGGATTCGG CACAAAATCA AAATGTAGCT CTAATTAAAA AGCAATTTCA TTTGTTATGG 
GGTCATCGGT GTATCAAAGT TTTATTGGCT GATTTTCTGA GGGATATTAA TGCTACTTAT 
GAGCGAATAG AATTCCAGCC CCCAGTACAA GCATCCACAA GCAACATACT TCGTCGCGCG 
TTGGACCGCG GTTCAGCTCG CGGTACTTTT ATCAGAGGCA ATAGAACATG GCGTCGATTA 
GATTGTATCG AGGTAGAGCA GGTGTCACGG GTATGCGCAA TGTTTTATGC GGCACTAAAT 
ACGCTTTCAC AACTTCGACT TGATATTTTA ACAGGTATTT GTTACAATGA TAATGTTTTG 
TACGATCTTT GGCTACTTCT CACTTCGCTG GGGCCGAATT GTGGCATGAA GGAGTTTATG 
GAGTTATTAA AGCAAGACAA CGTTTTACAA AAATCCCAGG TCACTATGCT CATGCTCTTT 
TGTGACAGTA TGACCCATTA TGTAACTATT TTGGATGAGT ATGAAATGTA CACAGAACAA 
AAACCATTTC TCCTCAATGA TTTTATAATG CTTACTTATT TTCTCAATAA TATACTGTAC 
AAACTAATTA ACGAGAATTT ACTCGGTACG AAAAATGTCA CCCAATGTCC GGTATTTGTA 
TCACTGCATA CGTTACTGTT ATGTCTATAC CGTCGTGATT GTCGTCGATC ATTTGCGCCA 
ACTAACCACT GGCTGATTCC CGAAGTAAAG CCCTCTGCAT TTATCAGTGA TTTGGAGAAA 
AACAAGAAAC ACGCAGTGAT TTTATTGCAG AAAATGCCAC ATATAATACC ACATGAAGAT 
CGCGTCAAAT TGTTTCGTAA ATTTGTACAA AATGAAAAGG CAGTGATGGG TTTGACGGAG 
AGTGCGTGTG CATCACCACG ATCTGCTTTA ATCGTCGTAC ATCGTGATCG CATTGTGGAG 
GATGGCTATA GACAGTTGGC AGCACTTAGG CCACATGCTC TGAAAGGCGT GATTCGTGTA 
CGTTTTATTA ATCAACAAGG TTTGCACGAG GCCGGTATTG ATCAAGATGG CGTTTTCAAG 
GAATTCTTAG AAGAAACAAT TAAGAGAGTA TTTGATCCAT CACTGAATTT ATTTAAAACC 
ACAACGGATC AGCGTTTATA TCCTTCGCCT ATATCATATG TACAGGACAA TCATTTGCAA 
TTGTTCGAGT TTGTAGGACG AATGTTGGGA AAAGCAGTAT ATGAAGGTAT AGTTGTGGAT 
GTTCCATTCG CTTCCTTCTT TCTCTCCCAG CTCTTAGGCC AAACACATCA GGCACTGTAC 
TCTTGTATGG ATGAGCTTCC CTCATTGGAC AACGAGCTCT ATCGCAGCCT TACTTTCATT 
AAGCACTATA AACAAGACGT TGCTGACTTG AATTTAACAT TTTCGGTAGA CCAAGATGTA 
ATGGGAAAGA TTGTTACACA TGAATTGCAT CCAGGTGGAA AAGCTCGTGC TGTTACTGAC 
AGCAACAAAC TAGTATACAT ACATTATATG GCCTATTTTC ATATGAACAC GCAAATACGA 
GAACAAACAC AGGCTTTCAA TCGTGGATTT CGTAGTATCG TCAACCCTGA ATGGTTATCC 
TTATTTTCAC CACCGGAATT ACAGCGCTTA ATATCTGGTG ATACTGTACC GCTGGATTTG 
CGAGATCTAC GAAAACATAC GCAGTACTAT GGAGGATTTC ATGATTCCCA CCGTGTCGTT 
GGTTGGTTAT GGGATATTTT GGCCAAAGAT TTTACGGAAG AAGAGCGGAA ATTATTTCTT 
AAGTTTGTTA CTAGTTGTTC CAAGCCACCT TTACTTGGAT TTGCCAATCT CGAGCCACCG 
TTTTCCATTC GTTGTGTTGA AGTTAGTGAT GACGAAGATA CTGGTGACAC TATTGGTAGT 
GTCATACGTG GTTTTTTTAC AATACGCAAG AAAGACCCCC TCAATCGGTT GCCGACATCA 
TCAACTTGCT TTAATTTATT GAAGTTGCCA AACTACCAAA AGAAGTCCAC GTTGCGCGAC 
AAATTACGTT ACGCTGTGAG CAGCAATACC GGATTTGAAT TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183777.1
CDS	201..3428
Translation	MFVTSDSEKKCFLEHTKAAREERAYEKRREEAAVKIQAHWKGYTTRKQYYNKIISDFDVIFIKENNDLDVDLLPSNTVYPVIRRFLTYFEYKNGSPTVVKPSREKFECLCRYLNKSMESESPKLSYAALCLHKDKSLPWLAHIKSLLSLCCLMLDDIKPENHTDSISLALYLHTLIVFTSPKSWGILRNKQFEKMQPVFQKICCNIQGKLVQNNFFKTMRSILLKGTAREDLSIKPVSLKAIMTLSTRPLIDGGYSRNLLTYFFAEILSVPALVFHLNIVTSQCLENLITMHILQRFVQSADDSNWFSEFTNSMPGTKLLAFLGNIVNLFTLESSVEAKELAYPLFTSATTILLENIPNTVSSRGVFTQWHELLGWHTPSLDSAQNQNVALIKKQFHLLWGHRCIKVLLADFLRDINATYERIEFQPPVQASTSNILRRALDRGSARGTFIRGNRTWRRLDCIEVEQVSRVCAMFYAALNTLSQLRLDILTGICYNDNVLYDLWLLLTSLGPNCGMKEFMELLKQDNVLQKSQVTMLMLFCDSMTHYVTILDEYEMYTEQKPFLLNDFIMLTYFLNNILYKLINENLLGTKNVTQCPVFVSLHTLLLCLYRRDCRRSFAPTNHWLIPEVKPSAFISDLEKNKKHAVILLQKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVVHRDRIVEDGYRQLAALRPHALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKRVFDPSLNLFKTTTDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTHQALYSCMDELPSLDNELYRSLTFIKHYKQDVADLNLTFSVDQDVMGKIVTHELHPGGKARAVTDSNKLVYIHYMAYFHMNTQIREQTQAFNRGFRSIVNPEWLSLFSPPELQRLISGDTVPLDLRDLRKHTQYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFANLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_011185475.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae ubiquitin-protein ligase E3B (LOC105213002), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185475.2

ORF Insert Sequence:

ATGTTTGTTA CGTCTGATTC GGAAAAAAAG TGTTTTTTGG AGCATACCAA AGCTGCTAGA 
GAGGAGCGTG CATACGAAAA ACGTCGGGAA GAAGCAGCTG TGAAAATACA AGCACACTGG 
AAAGGATATA CAACTCGTAA ACAGTATTAC AATAAGATAA TCTCCGACTT TGATGTTATA 
TTCATAAAGG AAAATAATGA TTTAGACGTG GATCTTTTAC CATCAAATAC CGTGTATCCG 
GTTATTCGTC GCTTTCTCAC ATATTTTGAA TATAAAAATG GATCGCCAAC CGTAGTGAAA 
CCGTCACGTG AAAAGTTTGA ATGTTTATGT CGTTATTTGA ACAAATCTAT GGAATCGGAA 
TCACCAAAAT TGTCATACGC AGCTCTTTGT CTGCACAAAG ATAAGAGCTT GCCTTGGTTG 
GCACATATCA AATCATTATT GAGTTTATGC TGTTTAATGC TGGACGATAT TAAGCCAGAA 
AATCATACGG ACAGCATTTC TCTGGCTCTG TATCTACATA CTTTGATTGT TTTCACTTCA 
CCGAAGTCAT GGGGTATTCT ACGAAATAAA CAATTCGAAA AAATGCAACC AGTATTTCAG 
AAAATATGCT GCAATATTCA AGGGAAATTA GTACAAAATA ATTTTTTCAA AACTATGCGC 
TCAATCCTAT TGAAGGGTAC AGCACGGGAG GATCTTTCCA TAAAACCAGT TTCCCTTAAA 
GCTATAATGA CGCTTTCTAC CCGACCGCTT ATCGATGGGG GCTATAGTCG TAATTTACTC 
ACATATTTTT TCGCCGAAAT TTTATCCGTT CCAGCATTGG TATTCCATTT GAATATTGTT 
ACCTCACAGT GCTTGGAAAA TCTCATTACC ATGCATATCC TCCAAAGATT TGTTCAATCA 
GCAGATGATT CTAATTGGTT TTCAGAATTT ACAAACAGTA TGCCCGGGAC GAAATTACTT 
GCATTTTTGG GGAATATTGT GAATCTTTTT ACACTCGAAA GCTCCGTGGA AGCAAAAGAG 
CTTGCATATC CTCTTTTCAC GAGTGCAACC ACTATACTTC TCGAAAATAT TCCTAACACG 
GTATCAAGTC GAGGAGTATT CACGCAATGG CATGAACTTC TTGGATGGCA TACTCCTTCG 
TTGGATTCGG CACAAAATCA AAATGTAGCT CTAATTAAAA AGCAATTTCA TTTGTTATGG 
GGTCATCGGT GTATCAAAGT TTTATTGGCT GATTTTCTGA GGGATATTAA TGCTACTTAT 
GAGCGAATAG AATTCCAGCC CCCAGTACAA GCATCCACAA GCAACATACT TCGTCGCGCG 
TTGGACCGCG GTTCAGCTCG CGGTACTTTT ATCAGAGGCA ATAGAACATG GCGTCGATTA 
GATTGTATCG AGGTAGAGCA GGTGTCACGG GTATGCGCAA TGTTTTATGC GGCACTAAAT 
ACGCTTTCAC AACTTCGACT TGATATTTTA ACAGGTATTT GTTACAATGA TAATGTTTTG 
TACGATCTTT GGCTACTTCT CACTTCGCTG GGGCCGAATT GTGGCATGAA GGAGTTTATG 
GAGTTATTAA AGCAAGACAA CGTTTTACAA AAATCCCAGG TCACTATGCT CATGCTCTTT 
TGTGACAGTA TGACCCATTA TGTAACTATT TTGGATGAGT ATGAAATGTA CACAGAACAA 
AAACCATTTC TCCTCAATGA TTTTATAATG CTTACTTATT TTCTCAATAA TATACTGTAC 
AAACTAATTA ACGAGAATTT ACTCGGTACG AAAAATGTCA CCCAATGTCC GGTATTTGTA 
TCACTGCATA CGTTACTGTT ATGTCTATAC CGTCGTGATT GTCGTCGATC ATTTGCGCCA 
ACTAACCACT GGCTGATTCC CGAAGTAAAG CCCTCTGCAT TTATCAGTGA TTTGGAGAAA 
AACAAGAAAC ACGCAGTGAT TTTATTGCAG AAAATGCCAC ATATAATACC ACATGAAGAT 
CGCGTCAAAT TGTTTCGTAA ATTTGTACAA AATGAAAAGG CAGTGATGGG TTTGACGGAG 
AGTGCGTGTG CATCACCACG ATCTGCTTTA ATCGTCGTAC ATCGTGATCG CATTGTGGAG 
GATGGCTATA GACAGTTGGC AGCACTTAGG CCACATGCTC TGAAAGGCGT GATTCGTGTA 
CGTTTTATTA ATCAACAAGG TTTGCACGAG GCCGGTATTG ATCAAGATGG CGTTTTCAAG 
GAATTCTTAG AAGAAACAAT TAAGAGAGTA TTTGATCCAT CACTGAATTT ATTTAAAACC 
ACAACGGATC AGCGTTTATA TCCTTCGCCT ATATCATATG TACAGGACAA TCATTTGCAA 
TTGTTCGAGT TTGTAGGACG AATGTTGGGA AAAGCAGTAT ATGAAGGTAT AGTTGTGGAT 
GTTCCATTCG CTTCCTTCTT TCTCTCCCAG CTCTTAGGCC AAACACATCA GGCACTGTAC 
TCTTGTATGG ATGAGCTTCC CTCATTGGAC AACGAGCTCT ATCGCAGCCT TACTTTCATT 
AAGCACTATA AACAAGACGT TGCTGACTTG AATTTAACAT TTTCGGTAGA CCAAGATGTA 
ATGGGAAAGA TTGTTACACA TGAATTGCAT CCAGGTGGAA AAGCTCGTGC TGTTACTGAC 
AGCAACAAAC TAGTATACAT ACATTATATG GCCTATTTTC ATATGAACAC GCAAATACGA 
GAACAAACAC AGGCTTTCAA TCGTGGATTT CGTAGTATCG TCAACCCTGA ATGGTTATCC 
TTATTTTCAC CACCGGAATT ACAGCGCTTA ATATCTGGTG ATACTGTACC GCTGGATTTG 
CGAGATCTAC GAAAACATAC GCAGTACTAT GGAGGATTTC ATGATTCCCA CCGTGTCGTT 
GGTTGGTTAT GGGATATTTT GGCCAAAGAT TTTACGGAAG AAGAGCGGAA ATTATTTCTT 
AAGTTTGTTA CTAGTTGTTC CAAGCCACCT TTACTTGGAT TTGCCAATCT CGAGCCACCG 
TTTTCCATTC GTTGTGTTGA AGTTAGTGAT GACGAAGATA CTGGTGACAC TATTGGTAGT 
GTCATACGTG GTTTTTTTAC AATACGCAAG AAAGACCCCC TCAATCGGTT GCCGACATCA 
TCAACTTGCT TTAATTTATT GAAGTTGCCA AACTACCAAA AGAAGTCCAC GTTGCGCGAC 
AAATTACGTT ACGCTGTGAG CAGCAATACC GGATTTGAAT TGTCCTAA